Mamatha Gavisiddaiah , Somashekar M. Metri , Santhepete Nanjundaiah Manjula , Kenganora Mruthunjaya , Shankar Thapa
{"title":"LC-MS研究神经递质在阿尔茨海默病中的调节作用：黑草乙醇提取物的保护作用","authors":"Mamatha Gavisiddaiah , Somashekar M. Metri , Santhepete Nanjundaiah Manjula , Kenganora Mruthunjaya , Shankar Thapa","doi":"10.1016/j.prenap.2025.100383","DOIUrl":null,"url":null,"abstract":"<div><h3>Introduction</h3><div>Alzheimer’s disease (AD) is characterized by significant alterations in neurotransmitter levels, leading to cognitive decline and neurodegeneration. This study investigates the potential protective role of <em>Nigella sativa</em> ethanolic extract in modulating neurotransmitters in an AD-induced animal model.</div></div><div><h3>Methods</h3><div>The phytochemical profiling of <em>N. sativa</em> extract was conducted through GC-MS/LC-MS analysis. LC-MS analysis of brain homogenates revealed that neurotransmitter levels, including acetylcholine (ACh), serotonin (5-HT), L-glutamic acid, and gamma-aminobutyric acid (GABA), were significantly reduced in the disease control (DC) group compared to the normal (N) group.</div></div><div><h3>Results</h3><div>We identified main phytoconstituents of <em>N. sativa</em> extract. Major components identified included thymoquinone, dithymoquinone, thymol, carvacrol, and α-hederin. Treatment with <em>N. sativa</em> extract demonstrated a dose-dependent restoration of neurotransmitter levels, with the high-dose group (NS2, 400 mg/kg) showing a more substantial effect than the low-dose group (NS1, 200 mg/kg). PCA analysis confirmed distinct clustering of groups, indicating metabolic alterations due to AD and restoration upon treatment. Statistical analysis confirmed significant improvements (<em>p < 0.05</em>) in neurotransmitter levels in treatment groups.</div></div><div><h3>Conclusion</h3><div>These findings suggest that <em>N. sativa</em> may offer neuroprotective benefits in AD by modulating neurotransmitter homeostasis.</div></div>","PeriodicalId":101014,"journal":{"name":"Pharmacological Research - Natural Products","volume":"9 ","pages":"Article 100383"},"PeriodicalIF":0.0000,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"LC-MS investigation of neurotransmitter modulation in Alzheimer’s disease: Protective role of ethanolic extract of Nigella sativa\",\"authors\":\"Mamatha Gavisiddaiah , Somashekar M. 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LC-MS analysis of brain homogenates revealed that neurotransmitter levels, including acetylcholine (ACh), serotonin (5-HT), L-glutamic acid, and gamma-aminobutyric acid (GABA), were significantly reduced in the disease control (DC) group compared to the normal (N) group.</div></div><div><h3>Results</h3><div>We identified main phytoconstituents of <em>N. sativa</em> extract. Major components identified included thymoquinone, dithymoquinone, thymol, carvacrol, and α-hederin. Treatment with <em>N. sativa</em> extract demonstrated a dose-dependent restoration of neurotransmitter levels, with the high-dose group (NS2, 400 mg/kg) showing a more substantial effect than the low-dose group (NS1, 200 mg/kg). PCA analysis confirmed distinct clustering of groups, indicating metabolic alterations due to AD and restoration upon treatment. Statistical analysis confirmed significant improvements (<em>p < 0.05</em>) in neurotransmitter levels in treatment groups.</div></div><div><h3>Conclusion</h3><div>These findings suggest that <em>N. sativa</em> may offer neuroprotective benefits in AD by modulating neurotransmitter homeostasis.</div></div>\",\"PeriodicalId\":101014,\"journal\":{\"name\":\"Pharmacological Research - Natural Products\",\"volume\":\"9 \",\"pages\":\"Article 100383\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2025-09-23\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Pharmacological Research - Natural Products\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2950199725002435\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Pharmacological Research - Natural Products","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2950199725002435","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
LC-MS investigation of neurotransmitter modulation in Alzheimer’s disease: Protective role of ethanolic extract of Nigella sativa
Introduction
Alzheimer’s disease (AD) is characterized by significant alterations in neurotransmitter levels, leading to cognitive decline and neurodegeneration. This study investigates the potential protective role of Nigella sativa ethanolic extract in modulating neurotransmitters in an AD-induced animal model.
Methods
The phytochemical profiling of N. sativa extract was conducted through GC-MS/LC-MS analysis. LC-MS analysis of brain homogenates revealed that neurotransmitter levels, including acetylcholine (ACh), serotonin (5-HT), L-glutamic acid, and gamma-aminobutyric acid (GABA), were significantly reduced in the disease control (DC) group compared to the normal (N) group.
Results
We identified main phytoconstituents of N. sativa extract. Major components identified included thymoquinone, dithymoquinone, thymol, carvacrol, and α-hederin. Treatment with N. sativa extract demonstrated a dose-dependent restoration of neurotransmitter levels, with the high-dose group (NS2, 400 mg/kg) showing a more substantial effect than the low-dose group (NS1, 200 mg/kg). PCA analysis confirmed distinct clustering of groups, indicating metabolic alterations due to AD and restoration upon treatment. Statistical analysis confirmed significant improvements (p < 0.05) in neurotransmitter levels in treatment groups.
Conclusion
These findings suggest that N. sativa may offer neuroprotective benefits in AD by modulating neurotransmitter homeostasis.
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