配对血清和脑脊液样品中总硒和硒种的生物标志物

IF 3.6 3区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
Teresa Urbano , Tommaso Filippini , Barbara R. Cardoso , Lauren A. Wise , Giovanna Zamboni , Annalisa Chiari , Giulia Vinceti , Manuela Tondelli , Alessandro Marti , Marcella Malavolti , Marco Vinceti , Bernhard Michalke
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引用次数: 0

摘要

生物标志物用于评估硒(Se)种类和硒蛋白在中枢神经系统(CNS)中的暴露的有效性尚未得到很好的研究。方法在83名意大利轻度认知障碍患者中,我们使用阴离子交换色谱-电感耦合等离子体动力学反应细胞-质谱法估计配对血清和脑脊液(CSF)样品中的总硒和单一硒种浓度。在每种基质(血清和脑脊液)中,我们评估了:1)配对硒种和2)总硒和硒种之间的相关性。结果该地区硒暴露分布与欧洲人群相当。我们发现大多数生物标志物(包括总硒和某些硒种)的模式很少一致。一个例外是硒蛋白-p结合的硒和无机硒形成硒酸盐之间的正相关,而谷胱甘肽-过氧化物酶结合的硒出人意料地呈负相关。血清中总硒与某些硒种呈正相关,与其他硒种呈负相关,而脑脊液中各硒种之间的正相关更强且更一致。结论血清中总硒和单种硒浓度与脑脊液中硒浓度无明显相关性,脑脊液是判断中枢神经系统暴露的金标准。此外,总硒和选定硒种在脑脊液中呈一致的正相关,而在血清中没有。我们的研究结果表明,依靠血清硒浓度来评估中枢神经系统暴露可能会在人类研究中引入错误。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Biomarkers of total selenium and selenium species in paired serum and cerebrospinal fluid samples

Background

The validity of biomarkers to estimate exposure to selenium (Se) species and selenoproteins in the central nervous system (CNS) is not well studied.

Methods

Among 83 Italian participants with mild cognitive impairment, we estimated total Se and single Se species concentrations in paired serum and cerebrospinal fluid (CSF) samples using anion exchange chromatography-inductively coupled plasma-dynamic reaction cell-mass spectrometry. In each matrix (serum and CSF), we assessed associations between: 1) paired Se species and 2) total Se and Se species.

Results

The distribution of Se exposure was comparable to that generally found in European populations. We found few consistent patterns for most biomarkers, including total Se and some Se species. An exception was a positive association between the two matrices for selenoprotein-P-bound Se and the inorganic Se form selenate, and an unexpected inverse association for glutathione-peroxidase-bound Se. Total Se was positively associated with some Se species but inversely associated with other Se species in serum, while in CSF the positive association was stronger and more consistent across various Se species.

Conclusions

Concentrations of total Se and single Se species in serum were not strongly correlated with their respective concentrations in CSF, the gold standard to estimate CNS exposure. Furthermore, total Se and selected Se species showed consistent positive correlations within CSF but not serum. Our results suggest that relying on serum Se concentrations to assess CNS exposure can introduce error in human studies.
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来源期刊
CiteScore
6.60
自引率
2.90%
发文量
202
审稿时长
85 days
期刊介绍: The journal provides the reader with a thorough description of theoretical and applied aspects of trace elements in medicine and biology and is devoted to the advancement of scientific knowledge about trace elements and trace element species. Trace elements play essential roles in the maintenance of physiological processes. During the last decades there has been a great deal of scientific investigation about the function and binding of trace elements. The Journal of Trace Elements in Medicine and Biology focuses on the description and dissemination of scientific results concerning the role of trace elements with respect to their mode of action in health and disease and nutritional importance. Progress in the knowledge of the biological role of trace elements depends, however, on advances in trace elements chemistry. Thus the Journal of Trace Elements in Medicine and Biology will include only those papers that base their results on proven analytical methods. Also, we only publish those articles in which the quality assurance regarding the execution of experiments and achievement of results is guaranteed.
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