{"title":"褪黑素对bps诱导的卵巢毒性的保护作用涉及自噬和自噬调节","authors":"Lina Chouchene, Kaouthar Kessabi, Lobna Lajmi, Imed Messaoudi","doi":"10.1016/j.reprotox.2025.109072","DOIUrl":null,"url":null,"abstract":"<div><div>Plastics pose a global health risk due to their detrimental effects, with bisphenol S (BPS) being a prominent plasticizer of concern. In this study, we aimed to investigate the effects of BPS exposure on ovarian function in Wistar rats and assess the protective potential of melatonin (MLT), with the goal of elucidating some of the underlying biochemical and molecular mechanisms involved. We first assessed changes in weight parameters and oxidative stress markers, including antioxidant enzymes (SOD and CAT), PSH, and MDA levels. Then, we investigated ovarian function parameters, namely those related to the estrous cycle, ovarian histology, folliculogenesis, and the molecular expression of markers associated with autophagy (LC3, P62, ATG5) and mitophagy (PINK1, PARKIN) processes. Our findings indicate that BPS induces oxidative stress, with increased CAT activity and decreased SOD activity, along with elevated PSH levels, without any effect on lipid peroxidation. Significant ovarian toxicity is also evident, manifested by a decrease in ovarian weight and anomalies related to the estrous cycle, such as irregularities and disruptions in the total duration and the different estrous phases, along with histological alterations in ovarian tissue that result in variations in the number of follicles at different maturation stages. Furthermore, BPS exposure induces an overexpression of LC3, ATG5, P62, PINK1 and PARKIN markers. Conversely, MLT supplementation significantly mitigated these effects, mainly through its ability to improve oxidative status and to modulate autophagy and mitophagy processes, resulting in the restoration of ovarian function parameters and highlighting its protective role against BPS-induced ovarian toxicity.</div></div>","PeriodicalId":21137,"journal":{"name":"Reproductive toxicology","volume":"138 ","pages":"Article 109072"},"PeriodicalIF":2.8000,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Involvement of autophagy and mitophagy modulation in the protective effect of melatonin against BPS-induced ovarian toxicity\",\"authors\":\"Lina Chouchene, Kaouthar Kessabi, Lobna Lajmi, Imed Messaoudi\",\"doi\":\"10.1016/j.reprotox.2025.109072\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Plastics pose a global health risk due to their detrimental effects, with bisphenol S (BPS) being a prominent plasticizer of concern. In this study, we aimed to investigate the effects of BPS exposure on ovarian function in Wistar rats and assess the protective potential of melatonin (MLT), with the goal of elucidating some of the underlying biochemical and molecular mechanisms involved. We first assessed changes in weight parameters and oxidative stress markers, including antioxidant enzymes (SOD and CAT), PSH, and MDA levels. Then, we investigated ovarian function parameters, namely those related to the estrous cycle, ovarian histology, folliculogenesis, and the molecular expression of markers associated with autophagy (LC3, P62, ATG5) and mitophagy (PINK1, PARKIN) processes. Our findings indicate that BPS induces oxidative stress, with increased CAT activity and decreased SOD activity, along with elevated PSH levels, without any effect on lipid peroxidation. Significant ovarian toxicity is also evident, manifested by a decrease in ovarian weight and anomalies related to the estrous cycle, such as irregularities and disruptions in the total duration and the different estrous phases, along with histological alterations in ovarian tissue that result in variations in the number of follicles at different maturation stages. Furthermore, BPS exposure induces an overexpression of LC3, ATG5, P62, PINK1 and PARKIN markers. Conversely, MLT supplementation significantly mitigated these effects, mainly through its ability to improve oxidative status and to modulate autophagy and mitophagy processes, resulting in the restoration of ovarian function parameters and highlighting its protective role against BPS-induced ovarian toxicity.</div></div>\",\"PeriodicalId\":21137,\"journal\":{\"name\":\"Reproductive toxicology\",\"volume\":\"138 \",\"pages\":\"Article 109072\"},\"PeriodicalIF\":2.8000,\"publicationDate\":\"2025-09-24\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Reproductive toxicology\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0890623825002436\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"REPRODUCTIVE BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Reproductive toxicology","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0890623825002436","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"REPRODUCTIVE BIOLOGY","Score":null,"Total":0}
Involvement of autophagy and mitophagy modulation in the protective effect of melatonin against BPS-induced ovarian toxicity
Plastics pose a global health risk due to their detrimental effects, with bisphenol S (BPS) being a prominent plasticizer of concern. In this study, we aimed to investigate the effects of BPS exposure on ovarian function in Wistar rats and assess the protective potential of melatonin (MLT), with the goal of elucidating some of the underlying biochemical and molecular mechanisms involved. We first assessed changes in weight parameters and oxidative stress markers, including antioxidant enzymes (SOD and CAT), PSH, and MDA levels. Then, we investigated ovarian function parameters, namely those related to the estrous cycle, ovarian histology, folliculogenesis, and the molecular expression of markers associated with autophagy (LC3, P62, ATG5) and mitophagy (PINK1, PARKIN) processes. Our findings indicate that BPS induces oxidative stress, with increased CAT activity and decreased SOD activity, along with elevated PSH levels, without any effect on lipid peroxidation. Significant ovarian toxicity is also evident, manifested by a decrease in ovarian weight and anomalies related to the estrous cycle, such as irregularities and disruptions in the total duration and the different estrous phases, along with histological alterations in ovarian tissue that result in variations in the number of follicles at different maturation stages. Furthermore, BPS exposure induces an overexpression of LC3, ATG5, P62, PINK1 and PARKIN markers. Conversely, MLT supplementation significantly mitigated these effects, mainly through its ability to improve oxidative status and to modulate autophagy and mitophagy processes, resulting in the restoration of ovarian function parameters and highlighting its protective role against BPS-induced ovarian toxicity.
期刊介绍:
Drawing from a large number of disciplines, Reproductive Toxicology publishes timely, original research on the influence of chemical and physical agents on reproduction. Written by and for obstetricians, pediatricians, embryologists, teratologists, geneticists, toxicologists, andrologists, and others interested in detecting potential reproductive hazards, the journal is a forum for communication among researchers and practitioners. Articles focus on the application of in vitro, animal and clinical research to the practice of clinical medicine.
All aspects of reproduction are within the scope of Reproductive Toxicology, including the formation and maturation of male and female gametes, sexual function, the events surrounding the fusion of gametes and the development of the fertilized ovum, nourishment and transport of the conceptus within the genital tract, implantation, embryogenesis, intrauterine growth, placentation and placental function, parturition, lactation and neonatal survival. Adverse reproductive effects in males will be considered as significant as adverse effects occurring in females. To provide a balanced presentation of approaches, equal emphasis will be given to clinical and animal or in vitro work. Typical end points that will be studied by contributors include infertility, sexual dysfunction, spontaneous abortion, malformations, abnormal histogenesis, stillbirth, intrauterine growth retardation, prematurity, behavioral abnormalities, and perinatal mortality.