褪黑素对bps诱导的卵巢毒性的保护作用涉及自噬和自噬调节

IF 2.8 4区 医学 Q2 REPRODUCTIVE BIOLOGY
Lina Chouchene, Kaouthar Kessabi, Lobna Lajmi, Imed Messaoudi
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引用次数: 0

摘要

塑料因其有害影响而对全球健康构成威胁,双酚S (BPS)是一种值得关注的突出增塑剂。在这项研究中,我们旨在研究BPS暴露对Wistar大鼠卵巢功能的影响,并评估褪黑激素(MLT)的保护潜力,目的是阐明一些潜在的生化和分子机制。我们首先评估了体重参数和氧化应激标志物的变化,包括抗氧化酶(SOD和CAT)、PSH和MDA水平。然后,我们研究了卵巢功能参数,即与发情周期、卵巢组织学、卵泡发生相关的参数,以及与自噬(LC3、P62、ATG5)和有丝分裂(PINK1、PARKIN)过程相关的标志物的分子表达。我们的研究结果表明,BPS诱导氧化应激,使CAT活性增加,SOD活性降低,PSH水平升高,而对脂质过氧化没有任何影响。显著的卵巢毒性也很明显,表现为卵巢重量的减少和与发情周期相关的异常,如总持续时间和不同发情期的不规则和中断,以及卵巢组织的组织学改变,导致不同成熟阶段卵泡数量的变化。此外,BPS暴露诱导LC3、ATG5、P62、PINK1和PARKIN标记物的过表达。相反,补充MLT显著减轻了这些影响,主要是通过其改善氧化状态和调节自噬和有丝自噬过程的能力,从而恢复卵巢功能参数,并突出其对bps诱导的卵巢毒性的保护作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Involvement of autophagy and mitophagy modulation in the protective effect of melatonin against BPS-induced ovarian toxicity
Plastics pose a global health risk due to their detrimental effects, with bisphenol S (BPS) being a prominent plasticizer of concern. In this study, we aimed to investigate the effects of BPS exposure on ovarian function in Wistar rats and assess the protective potential of melatonin (MLT), with the goal of elucidating some of the underlying biochemical and molecular mechanisms involved. We first assessed changes in weight parameters and oxidative stress markers, including antioxidant enzymes (SOD and CAT), PSH, and MDA levels. Then, we investigated ovarian function parameters, namely those related to the estrous cycle, ovarian histology, folliculogenesis, and the molecular expression of markers associated with autophagy (LC3, P62, ATG5) and mitophagy (PINK1, PARKIN) processes. Our findings indicate that BPS induces oxidative stress, with increased CAT activity and decreased SOD activity, along with elevated PSH levels, without any effect on lipid peroxidation. Significant ovarian toxicity is also evident, manifested by a decrease in ovarian weight and anomalies related to the estrous cycle, such as irregularities and disruptions in the total duration and the different estrous phases, along with histological alterations in ovarian tissue that result in variations in the number of follicles at different maturation stages. Furthermore, BPS exposure induces an overexpression of LC3, ATG5, P62, PINK1 and PARKIN markers. Conversely, MLT supplementation significantly mitigated these effects, mainly through its ability to improve oxidative status and to modulate autophagy and mitophagy processes, resulting in the restoration of ovarian function parameters and highlighting its protective role against BPS-induced ovarian toxicity.
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来源期刊
Reproductive toxicology
Reproductive toxicology 生物-毒理学
CiteScore
6.50
自引率
3.00%
发文量
131
审稿时长
45 days
期刊介绍: Drawing from a large number of disciplines, Reproductive Toxicology publishes timely, original research on the influence of chemical and physical agents on reproduction. Written by and for obstetricians, pediatricians, embryologists, teratologists, geneticists, toxicologists, andrologists, and others interested in detecting potential reproductive hazards, the journal is a forum for communication among researchers and practitioners. Articles focus on the application of in vitro, animal and clinical research to the practice of clinical medicine. All aspects of reproduction are within the scope of Reproductive Toxicology, including the formation and maturation of male and female gametes, sexual function, the events surrounding the fusion of gametes and the development of the fertilized ovum, nourishment and transport of the conceptus within the genital tract, implantation, embryogenesis, intrauterine growth, placentation and placental function, parturition, lactation and neonatal survival. Adverse reproductive effects in males will be considered as significant as adverse effects occurring in females. To provide a balanced presentation of approaches, equal emphasis will be given to clinical and animal or in vitro work. Typical end points that will be studied by contributors include infertility, sexual dysfunction, spontaneous abortion, malformations, abnormal histogenesis, stillbirth, intrauterine growth retardation, prematurity, behavioral abnormalities, and perinatal mortality.
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