牛乳及血清Fas2-ELISA检测牛肝片形吸虫感染的评价

IF 2.2 2区农林科学 Q2 PARASITOLOGY

Veterinary parasitology Pub Date : 2025-09-22 DOI:10.1016/j.vetpar.2025.110615

MP Garcia-Lazaro , E. Chavarry , D. Zarate-Rendón , P. Herrera-Velit , JR Espinoza

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引用次数: 0

摘要

肝片吸虫在全世界的养牛业中造成奶和肉生产的重大损失。在感染高发地区饲养的牛中，治疗和控制甲型肝炎感染需要敏感、特异和负担得起的诊断检测。本研究旨在评价Fas2-ELISA和ES-ELISA两种检测方法对秘鲁两个畜牧盆地肉牛和奶牛片吸虫病的诊断效果。从利马一个屠宰场屠宰的肉牛身上采集了血清和粪便样本；从位于曼塔罗安第斯山谷的圣洛伦佐区自然感染肝原孢菌的奶牛和利马拉莫利纳区大学马厩饲养的奶牛中采集血清、牛奶和粪便样本。ES- elisa检测成年吸虫体内针对肝F.排泄-分泌产物（ES）的IgG抗体，Fas2- elisa检测从成年吸虫体内纯化的半胱氨酸蛋白酶Fas2抗原。以肝吸虫检测为参考指标，确定肉牛的诊断参数。ES-ELISA检测肉牛肝原胞菌感染的灵敏度（Se）为93.2 %(95 % CI, 83.5 ~ 98.1 %)，特异性（Sp）为88 %（95 % CI, 80.4 ~ 93.5 %）。Fas2-ELISA检测结果显示Se和Sp分别为98.3% %（95 % CI, 91 ~ 99 %）和93.6 %（95 % CI, 87 ~ 97 %）。诊断参数对牛奶样品中检测f .植物感染100年ES-ELISA显示Se %(95 % CI, 92 - 100 %)和Sp 83 %(95 % CI, 65 - 94 %),Fas2-ELISA显示98 Se %(95 % CI, 89 - 100 %)和Sp 93 %(95 % CI, 78 - 99 %)测试使用coproscopy作为参考。这些结果表明，Fas2-ELISA可作为一种有效的检测奶牛乳样品中肝芽胞杆菌感染的检测方法，并可进一步发展为一种估计奶牛群中肝芽胞杆菌感染流行率的大罐乳（BTM）检测方法。

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Evaluation of Fas2-ELISA in milk and serum for the detection of Fasciola hepatica infection in cattle

Fasciola hepatica causes a significant loss in milk and meat production in cattle farming worldwide. The treatment and control of F. hepatica infection in cattle raised in regions with a high prevalence of the infection requires sensitive, specific, and affordable diagnostic tests. This study aims to evaluate two tests, Fas2-ELISA and ES-ELISA, for the diagnosis of fascioliasis in beef and dairy cattle from two livestock basins in Peru. Serum and fecal samples were collected from beef cattle slaughtered at an abattoir in Lima; serum, milk, and fecal samples were collected from F. hepatica naturally infected dairy cows in the San Lorenzo district, located in the Mantaro Andean Valley, and dairy cows raised in university stables in La Molina district in Lima. ES-ELISA detects IgG antibodies against F. hepatica excretory-secretory products (ES) from adult flukes, and Fas2-ELISA detects the Fas2 antigen, a cysteine proteinase purified from F. hepatica adult flukes. The diagnostic parameters for beef cattle were determined using inspection for F. hepatica flukes in the liver as a reference test. ES-ELISA for the detection of F. hepatica infection in beef cattle showed a sensitivity (Se) of 93.2 % (95 % CI, 83.5–98.1 %) and specificity (Sp) of 88 % (95 % CI, 80.4–93.5 %). Fas2-ELISA showed a Se and Sp of 98.3 % (95 % CI, 91–99 %) and 93.6 % (95 % CI, 87–97 %), respectively. The diagnostic parameters for detecting F. hepatica infection in milk samples by ES-ELISA showed a Se of 100 % (95 % CI, 92–100 %) and Sp of 83 % (95 % CI, 65–94 %), Fas2-ELISA showed a Se of 98 % (95 % CI, 89–100 %) and Sp of 93 % (95 % CI, 78–99 %) using coproscopy as a reference test. These results suggest that Fas2-ELISA could be a useful test for detecting F. hepatica infection in milk samples from dairy cattle and may be further developed as a bulk-tank milk (BTM) test to estimate the prevalence of F. hepatica infection in dairy herds.

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来源期刊

Veterinary parasitology 农林科学-寄生虫学

CiteScore

5.30

自引率

7.70%

发文量

126

审稿时长

36 days

期刊介绍： The journal Veterinary Parasitology has an open access mirror journal,Veterinary Parasitology: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review. This journal is concerned with those aspects of helminthology, protozoology and entomology which are of interest to animal health investigators, veterinary practitioners and others with a special interest in parasitology. Papers of the highest quality dealing with all aspects of disease prevention, pathology, treatment, epidemiology, and control of parasites in all domesticated animals, fall within the scope of the journal. Papers of geographically limited (local) interest which are not of interest to an international audience will not be accepted. Authors who submit papers based on local data will need to indicate why their paper is relevant to a broader readership. Parasitological studies on laboratory animals fall within the scope of the journal only if they provide a reasonably close model of a disease of domestic animals. Additionally the journal will consider papers relating to wildlife species where they may act as disease reservoirs to domestic animals, or as a zoonotic reservoir. Case studies considered to be unique or of specific interest to the journal, will also be considered on occasions at the Editors'' discretion. Papers dealing exclusively with the taxonomy of parasites do not fall within the scope of the journal.