白弧菌素对分钟树蝇蝌蚪毒性的实验研究

IF 2.4 4区医学 Q2 PHARMACOLOGY & PHARMACY

Toxicon Pub Date : 2025-09-25 DOI:10.1016/j.toxicon.2025.108600

Monica Chacon de Vicente , Luiza Gabriella Ferreira de Paula , Renata Maria Pereira de Freitas , Adieli dos Anjos Almeida , Diego da Silva Lima , Rebeca dos Santos Gabriel , Nathany Geraldino Nogueira , Nádya Raquel dos Santos Batista , Alexandre Có Mangoni Barros , Marcelino Benvindo de Souza , Rogério Pereira Bastos , Pedro Vale de Azevedo Brito , Daniela de Melo e Silva , Ana Flávia Machado Botelho

{"title":"白弧菌素对分钟树蝇蝌蚪毒性的实验研究","authors":"Monica Chacon de Vicente , Luiza Gabriella Ferreira de Paula , Renata Maria Pereira de Freitas , Adieli dos Anjos Almeida , Diego da Silva Lima , Rebeca dos Santos Gabriel , Nathany Geraldino Nogueira , Nádya Raquel dos Santos Batista , Alexandre Có Mangoni Barros , Marcelino Benvindo de Souza , Rogério Pereira Bastos , Pedro Vale de Azevedo Brito , Daniela de Melo e Silva , Ana Flávia Machado Botelho","doi":"10.1016/j.toxicon.2025.108600","DOIUrl":null,"url":null,"abstract":"<div><div>Beauvericin (BEA) is an emerging mycotoxin frequently detected in food and feed, raising concerns about its toxicological risks for One Health. This study evaluated the acute toxicity of BEA in tadpoles of <em>Dendropsophus minutus</em>, a bioindicator species of environmental toxicity, after a 96-h exposure. Tadpoles were exposed to three concentrations of BEA, with vehicle (DMSO), positive (cyclophosphamide), and negative (water) controls. Outcomes included survival, genotoxicity (comet assay), mutagenicity (micronucleus test), leukocyte profiles, redox status markers, and liver histopathology. Exposure to BEA did not induce genotoxicity; however, it resulted in mutagenic effects, with an increase in reniform nuclei and a decrease in anucleated erythrocytes, and hepatotoxicity characterized by sinusoidal congestion, reduced hepatocyte cytoplasmic volume, and increased connective tissue. Elevated levels of nitric oxide and carbonyl proteins were observed, indicating oxidative stress, while antioxidant enzymes and acetylcholinesterase activity remained unchanged. These findings demonstrate that BEA induces mutagenic, cytotoxic, and hepatotoxic effects in amphibians, highlighting its potential ecological impact and relevance for One Health risk assessment.</div></div>","PeriodicalId":23289,"journal":{"name":"Toxicon","volume":"267 ","pages":"Article 108600"},"PeriodicalIF":2.4000,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Experimental assessment of beauvericin toxicity in tadpoles of Dendropsophus minutus (Anura: Hylidae)\",\"authors\":\"Monica Chacon de Vicente , Luiza Gabriella Ferreira de Paula , Renata Maria Pereira de Freitas , Adieli dos Anjos Almeida , Diego da Silva Lima , Rebeca dos Santos Gabriel , Nathany Geraldino Nogueira , Nádya Raquel dos Santos Batista , Alexandre Có Mangoni Barros , Marcelino Benvindo de Souza , Rogério Pereira Bastos , Pedro Vale de Azevedo Brito , Daniela de Melo e Silva , Ana Flávia Machado Botelho\",\"doi\":\"10.1016/j.toxicon.2025.108600\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Beauvericin (BEA) is an emerging mycotoxin frequently detected in food and feed, raising concerns about its toxicological risks for One Health. This study evaluated the acute toxicity of BEA in tadpoles of <em>Dendropsophus minutus</em>, a bioindicator species of environmental toxicity, after a 96-h exposure. Tadpoles were exposed to three concentrations of BEA, with vehicle (DMSO), positive (cyclophosphamide), and negative (water) controls. Outcomes included survival, genotoxicity (comet assay), mutagenicity (micronucleus test), leukocyte profiles, redox status markers, and liver histopathology. Exposure to BEA did not induce genotoxicity; however, it resulted in mutagenic effects, with an increase in reniform nuclei and a decrease in anucleated erythrocytes, and hepatotoxicity characterized by sinusoidal congestion, reduced hepatocyte cytoplasmic volume, and increased connective tissue. Elevated levels of nitric oxide and carbonyl proteins were observed, indicating oxidative stress, while antioxidant enzymes and acetylcholinesterase activity remained unchanged. These findings demonstrate that BEA induces mutagenic, cytotoxic, and hepatotoxic effects in amphibians, highlighting its potential ecological impact and relevance for One Health risk assessment.</div></div>\",\"PeriodicalId\":23289,\"journal\":{\"name\":\"Toxicon\",\"volume\":\"267 \",\"pages\":\"Article 108600\"},\"PeriodicalIF\":2.4000,\"publicationDate\":\"2025-09-25\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Toxicon\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0041010125003757\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"PHARMACOLOGY & PHARMACY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Toxicon","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0041010125003757","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"PHARMACOLOGY & PHARMACY","Score":null,"Total":0}

引用次数: 0

摘要

Beauvericin （BEA）是一种新兴的真菌毒素，经常在食品和饲料中检测到，引起人们对其毒理学风险的关注。本研究评估了BEA对环境毒性生物指标物种分钟树熊蝌蚪暴露96 h后的急性毒性。将蝌蚪暴露于三种浓度的BEA中，分别为对照剂（DMSO）、阳性对照（环磷酰胺）和阴性对照（水）。结果包括生存、遗传毒性（彗星试验）、致突变性（微核试验）、白细胞谱、氧化还原状态标记物和肝脏组织病理学。暴露于BEA没有引起遗传毒性；然而，它具有致突变作用，肾形核增加，无核红细胞减少，肝毒性表现为肝窦充血，肝细胞胞浆体积减少，结缔组织增加。观察到一氧化氮和羰基蛋白水平升高，表明氧化应激，而抗氧化酶和乙酰胆碱酯酶活性保持不变。这些发现表明BEA在两栖动物中诱导诱变、细胞毒性和肝毒性作用，突出了其潜在的生态影响和与One Health风险评估的相关性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Experimental assessment of beauvericin toxicity in tadpoles of Dendropsophus minutus (Anura: Hylidae)

查看原文本刊更多论文

Experimental assessment of beauvericin toxicity in tadpoles of Dendropsophus minutus (Anura: Hylidae)

Beauvericin (BEA) is an emerging mycotoxin frequently detected in food and feed, raising concerns about its toxicological risks for One Health. This study evaluated the acute toxicity of BEA in tadpoles of Dendropsophus minutus, a bioindicator species of environmental toxicity, after a 96-h exposure. Tadpoles were exposed to three concentrations of BEA, with vehicle (DMSO), positive (cyclophosphamide), and negative (water) controls. Outcomes included survival, genotoxicity (comet assay), mutagenicity (micronucleus test), leukocyte profiles, redox status markers, and liver histopathology. Exposure to BEA did not induce genotoxicity; however, it resulted in mutagenic effects, with an increase in reniform nuclei and a decrease in anucleated erythrocytes, and hepatotoxicity characterized by sinusoidal congestion, reduced hepatocyte cytoplasmic volume, and increased connective tissue. Elevated levels of nitric oxide and carbonyl proteins were observed, indicating oxidative stress, while antioxidant enzymes and acetylcholinesterase activity remained unchanged. These findings demonstrate that BEA induces mutagenic, cytotoxic, and hepatotoxic effects in amphibians, highlighting its potential ecological impact and relevance for One Health risk assessment.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Toxicon 医学-毒理学

CiteScore

4.80

自引率

10.70%

发文量

358

审稿时长

68 days

期刊介绍： Toxicon has an open access mirror Toxicon: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review. An introductory offer Toxicon: X - full waiver of the Open Access fee. Toxicon''s "aims and scope" are to publish: -articles containing the results of original research on problems related to toxins derived from animals, plants and microorganisms -papers on novel findings related to the chemical, pharmacological, toxicological, and immunological properties of natural toxins -molecular biological studies of toxins and other genes from poisonous and venomous organisms that advance understanding of the role or function of toxins -clinical observations on poisoning and envenoming where a new therapeutic principle has been proposed or a decidedly superior clinical result has been obtained. -material on the use of toxins as tools in studying biological processes and material on subjects related to venom and antivenom problems. -articles on the translational application of toxins, for example as drugs and insecticides -epidemiological studies on envenoming or poisoning, so long as they highlight a previously unrecognised medical problem or provide insight into the prevention or medical treatment of envenoming or poisoning. Retrospective surveys of hospital records, especially those lacking species identification, will not be considered for publication. Properly designed prospective community-based surveys are strongly encouraged. -articles describing well-known activities of venoms, such as antibacterial, anticancer, and analgesic activities of arachnid venoms, without any attempt to define the mechanism of action or purify the active component, will not be considered for publication in Toxicon. -review articles on problems related to toxinology. To encourage the exchange of ideas, sections of the journal may be devoted to Short Communications, Letters to the Editor and activities of the affiliated societies.