受阿留申水貂病病毒感染的水貂脾脏转录组学分析：对免疫反应的洞察

IF 1.8 3区农林科学 Q1 VETERINARY SCIENCES

Research in veterinary science Pub Date : 2025-08-12 DOI:10.1016/j.rvsc.2025.105842

D. Gregory Bishop , A. Hossain Farid , Duy Ngoc Do , Younes Miar

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引用次数: 0

摘要

阿留申病（AD）是最具挑战性的水貂疾病之一，它导致高死亡率并影响几个重要的经济性状。阿留申水貂病病毒（AMDV）的目标是多个器官，脾脏是主要的目标器官之一。转录组学已被广泛用于揭示基因和生物学途径，并确定早期检测或预防疾病的生物标志物。本研究旨在利用转录组学方法鉴定与宿主对脾脏AD感染反应相关的基因和途径。为此，12只无amdv的黑公水貂（3组，每组4只全兄妹）在接种前饲养4 - 7天，然后在感染后24 h（第1天）、48 h（第2天）和第7天用病毒匀浆鼻内接种并安乐死。采集感染水貂和对照组（第0天）脾脏组织进行RNA分离。文库制备（Illumina TruSeq™RNA试剂盒）并测序（HiScanSQ， 101 bp配对末端reads）。在使用Cutadapt v1.4.2清洗后，仍有942,803,540个高质量读数可供分析。差异基因表达分析显示，第1天至第0天，显著差异表达（DE）基因数量最多（168个），第7天至第0天，显著差异表达基因数量最少（23个）。三组观察对比较共鉴定出19个DE基因，其中8个直接参与免疫应答（FGL2、TLF8、LRP1、SERPINB9、MSR1、C3、PLA2R1和XCR1）。基因富集分析揭示了与先天免疫和脂质代谢相关的途径，这是宿主抵抗感染的重要机制。其中一个重要的途径是中性粒细胞脱芽，它的功能是帮助消除病原体和启动炎症过程。总之，目前的研究提供了对感染AMDV的水貂脾脏转录组谱的深入了解。所鉴定的候选基因可用于水貂抗阿尔茨海默病的功能研究或作为标记或基因组选择的先验信息。对其他组织或单细胞RNA测序的进一步研究可能会提供宿主对AMDV感染反应的更全面的图像。

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Transcriptomic profiling of mink spleen infected by Aleutian mink disease virus: Insight into immune response

Aleutian disease (AD) is one of the most challenging mink diseases that causes high mortality and affects several economically important traits. Aleutian mink disease virus (AMDV) targets multiple organs, the spleen being one of the major targeted organs. Transcriptomics has been widely used to reveal genes and biological pathways and to identify biomarkers for early detection or prevention of diseases. This study aimed to identify genes and pathways related to the host response to AD infection in the spleen using transcriptomics. For this purpose, twelve AMDV-free black male mink (3 sets of 4 full-sibs) were housed for 4–7 days pre-inoculation and then were intranasally inoculated with viral homogenate and euthanized at 24 h (Day 1), 48 h (Day 2), and Day 7 post-infection. Spleen tissues were collected from infected and control (Day 0) mink for RNA isolation. Libraries were prepared (Illumina TruSeq™ RNA kit) and sequenced (HiScanSQ, 101 bp paired-end reads). After cleaning with Cutadapt v1.4.2, 942,803,540 high-quality reads remained for analysis. Differential gene expression analyses revealed that the highest number of significant differentially expressed (DE) genes were observed between Day 1 and Day 0 (168) while the fewest DE genes (23) were observed between Day 7 and Day 0. A total of 19 DE genes are identified between three observation pair comparisons and eight of them are directly involved in immune response (FGL2, TLF8, LRP1, SERPINB9, MSR1, C3, PLA2R1, and XCR1). Gene enrichment analyses revealed pathways related to innate immune and lipid metabolism which are important mechanisms for the host to fight against infections. One of the important pathways is neutrophil degranulation which functions as aiding in the elimination of pathogens and the initiation of the inflammatory process. In conclusion, the current study provides insight into the transcriptomic profiles of the spleen in mink infected with AMDV. Identified candidate genes might be used for functional studies or as prior information for markers or genomic selection against AD in mink. Further studies in other tissues or single-cell RNA sequencing might deliver more comprehensive pictures of host responses to AMDV infections.

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来源期刊

Research in veterinary science 农林科学-兽医学

CiteScore

4.40

自引率

4.20%

发文量

312

审稿时长

75 days

期刊介绍： Research in Veterinary Science is an International multi-disciplinary journal publishing original articles, reviews and short communications of a high scientific and ethical standard in all aspects of veterinary and biomedical research. The primary aim of the journal is to inform veterinary and biomedical scientists of significant advances in veterinary and related research through prompt publication and dissemination. Secondly, the journal aims to provide a general multi-disciplinary forum for discussion and debate of news and issues concerning veterinary science. Thirdly, to promote the dissemination of knowledge to a broader range of professions, globally. High quality papers on all species of animals are considered, particularly those considered to be of high scientific importance and originality, and with interdisciplinary interest. The journal encourages papers providing results that have clear implications for understanding disease pathogenesis and for the development of control measures or treatments, as well as those dealing with a comparative biomedical approach, which represents a substantial improvement to animal and human health. Studies without a robust scientific hypothesis or that are preliminary, or of weak originality, as well as negative results, are not appropriate for the journal. Furthermore, observational approaches, case studies or field reports lacking an advancement in general knowledge do not fall within the scope of the journal.