泰国南部肉鸡生产链中分离的肠炎沙门氏菌的抗生素耐药性、序列分型和毒力基因谱

IF 1.8 3区农林科学 Q1 VETERINARY SCIENCES

Research in veterinary science Pub Date : 2025-09-16 DOI:10.1016/j.rvsc.2025.105891

Bussara Sripaurya , Wattana Pelyuntha , Ruttayaporn Ngasaman , Chai Lay Ching , Vincent Guyonnet , Kitiya Vongkamjan

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引用次数: 0

摘要

沙门氏菌是来自家禽养殖场的一种主要食源性病原体，在屠宰场加工或运往菜市场的过程中有可能转移到肉制品中。在总共351份样本中，仅73份（20.8%）样本呈沙门氏菌阳性，这些样本来自同一省内独立选取的肉鸡养殖场、屠宰场和菜市场的鸡肉摊位。对56株沙门氏菌分离株进行了9种已知毒力基因（sipB、prgH、spaN、orgA、tolC、sitC、sifA、cdtB和sopB）的检测和耐药性分析。模式II （cdtB阴性）是23株沙门氏菌分离株中最主要的模式。最常见的抗生素耐药谱是AMP-TE-CIP-NA，在所有来源的10株分离株中发现。利用多位点序列分型（MLST）分析分离到的沙门氏菌亚型间的关系，发现12种序列类型。最常检测到的序列类型是ST198(26.8%)，在南肯塔基州发现。在这项研究中，ST198在所有来源的6个多药耐药（MDR）谱中被鉴定出来，并与氨苄西林和环丙沙星耐药有关。总的来说，我们的研究表明，监测STs和抗生素耐药表型，以跟踪来自特定来源的沙门氏菌分离株的耐多药传播，包括在肉鸡价值链中，具有潜在的公共卫生效益。

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Antibiotic resistance, sequence typing, and virulence gene profiles of Salmonella enterica isolated from the broiler production chain in southern Thailand

Salmonella is a major foodborne pathogen from poultry farms and potentially transferred to meat products during processing in slaughterhouses or transportation to wet markets. From a total of 351 samples, only 73 samples (20.8 %) collected from broiler farms, slaughterhouses, and chicken meat stalls at a wet market, which were selected independently within the same province, were positive for Salmonella. Fifty-six Salmonella isolates were evaluated for the presence of nine known virulence genes (sipB, prgH, spaN, orgA, tolC, sitC, sifA, cdtB, and sopB) and their antibiotic resistance profiles. Pattern II (cdtB negative) represented the most predominant pattern found in 23 Salmonella isolates. The most common antibiotic resistance profile was AMP-TE-CIP-NA, found in 10 isolates from all sources. The relationship of subtypes of Salmonella spp. isolated, analyzed by multilocus sequence typing (MLST), revealed twelve sequence types (STs). The most frequently detected sequence type was ST198 (26.8 %), identified in S. Kentucky. In this study, ST198 was identified in six multidrug resistance (MDR) profiles from all sources and was associated with ampicillin and ciprofloxacin resistance. Overall, our study indicated the potential public health benefits of monitoring STs and antibiotic resistance phenotypes to track the spread of MDR in Salmonella isolates from specific sources, including within the broiler value chain.

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来源期刊

Research in veterinary science 农林科学-兽医学

CiteScore

4.40

自引率

4.20%

发文量

312

审稿时长

75 days

期刊介绍： Research in Veterinary Science is an International multi-disciplinary journal publishing original articles, reviews and short communications of a high scientific and ethical standard in all aspects of veterinary and biomedical research. The primary aim of the journal is to inform veterinary and biomedical scientists of significant advances in veterinary and related research through prompt publication and dissemination. Secondly, the journal aims to provide a general multi-disciplinary forum for discussion and debate of news and issues concerning veterinary science. Thirdly, to promote the dissemination of knowledge to a broader range of professions, globally. High quality papers on all species of animals are considered, particularly those considered to be of high scientific importance and originality, and with interdisciplinary interest. The journal encourages papers providing results that have clear implications for understanding disease pathogenesis and for the development of control measures or treatments, as well as those dealing with a comparative biomedical approach, which represents a substantial improvement to animal and human health. Studies without a robust scientific hypothesis or that are preliminary, or of weak originality, as well as negative results, are not appropriate for the journal. Furthermore, observational approaches, case studies or field reports lacking an advancement in general knowledge do not fall within the scope of the journal.