白藜芦醇对异丙肾上腺素（ISO）给药雌性去卵巢大鼠炎症和氧化应激介导的肾功能和心功能障碍的治疗作用

IF 7.5 2区医学 Q1 MEDICINE, RESEARCH & EXPERIMENTAL

Biomedicine & Pharmacotherapy Pub Date : 2025-09-27 DOI:10.1016/j.biopha.2025.118571

Tahmina Yasmin , Mirza Alimullah , Md Junaeid Rahman , Shamima Sultana , Shanaz Siddiqua , Ishrat Jahan , Sohel Rana , Nusrat Subhan , Ferdous Khan , Md Ashraful Alam , Nasrin Akhter

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引用次数: 0

摘要

本研究旨在评价白藜芦醇对异丙肾上腺素（ISO）给药雌性去卵巢大鼠肾脏和心脏功能障碍的保护作用。ISO剂量为50 mg/kg（每周两次）以诱导梗死样症状，白藜芦醇剂量为100 mg/kg，每天两次，连续两周。所有的动物都得到了标准的食物和随意的水。在第14天对所有大鼠进行超声心动图检查以获取功能数据。第15天处死大鼠，收集血浆和组织样本进行分析。评估各种标志物，包括丙二醛（MDA）、一氧化氮（NO）、高级氧化蛋白产物（AOPP）、过氧化氢酶、超氧化物歧化酶（SOD）、谷胱甘肽（GSH）、肌酸酐激酶-肌脑（CK-MB）、髓过氧化物酶（MPO）、肌酐和尿酸。这项研究表明，ISO损害抗氧化防御，而白藜芦醇治疗有助于恢复氧化还原平衡，防止心脏和肾脏损伤。白藜芦醇治疗保留了iso给药大鼠的射血分数（EF%），缩短分数（FS%），并降低了心脏质量。基因表达分析显示，ISO下调了关键的抗氧化介质，而上调了促炎基因。白藜芦醇逆转了这些作用，表明可以减少氧化应激和炎症。组织病理学分析支持白藜芦醇的保护作用，显示心脏和肾脏组织的组织损伤和胶原沉积减少。此外，网络药理学分析显示了白藜芦醇与关键分子途径的潜在相互作用。综上所述，白藜芦醇通过增强抗氧化防御和抑制炎症来减轻iso诱导的心脏和肾脏损伤，提示其在预防或治疗氧化应激相关心血管和肾脏疾病方面具有潜在的临床应用价值。

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Therapeutic role of resveratrol treatment on inflammation and oxidative stress-mediated renal and cardiac dysfunction in isoproterenol (ISO) administered ovariectomized female Long Evans rats

This study aimed to evaluate the protective effects of resveratrol on renal and cardiac dysfunction in isoproterenol (ISO) administered ovariectomized female Long Evans rats. ISO was administered at 50 mg/kg (twice weekly) to induce infarct-like symptoms, while resveratrol was given daily at 100 mg/kg for two weeks. All animals received a standard chow diet and water ad libitum. The echocardiography was done on day 14 for all rats to acquire functional data. On day 15, rats were sacrificed, and plasma and tissue samples were collected for analysis. Various markers, including malondialdehyde (MDA), nitric oxide (NO), advanced oxidation protein products (AOPP), catalase, superoxide dismutase (SOD), glutathione (GSH), creatinine kinase – muscle brain (CK-MB), myeloperoxidase (MPO), creatinine, and uric acid, were assessed. This investigation revealed that ISO impaired antioxidant defenses, while resveratrol treatment helped restore redox balance and prevented cardiac and renal injury. Resveratrol treatment preserved the ejection fraction (EF%), fractional shortening (FS%), and lowered cardiac mass in ISO-administered rats. Gene expression analysis revealed that ISO downregulated key antioxidant mediators, while upregulating the pro-inflammatory genes. Resveratrol reversed these effects, suggesting reduced oxidative stress and inflammation. The histopathological analysis supported the protective role of resveratrol, showing reduced tissue damage and collagen deposition in heart and kidney tissues. Moreover, network pharmacology analysis showed potential interactions with key molecular pathways for resveratrol. In conclusion, resveratrol mitigated ISO-induced cardiac and renal damage by enhancing antioxidant defenses and suppressing inflammation, suggesting its potential clinical application in preventing or treating oxidative stress-related cardiovascular and renal disorders.

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来源期刊

Biomedicine & Pharmacotherapy 医学-药学

CiteScore

11.90

自引率

2.70%

发文量

1621

审稿时长

48 days

期刊介绍： Biomedicine & Pharmacotherapy stands as a multidisciplinary journal, presenting a spectrum of original research reports, reviews, and communications in the realms of clinical and basic medicine, as well as pharmacology. The journal spans various fields, including Cancer, Nutriceutics, Neurodegenerative, Cardiac, and Infectious Diseases.