通过耦合非酶DNA助行器到葡萄糖/氧生物燃料电池的灵敏自供电赭曲霉毒素A检测

IF 4.1 3区化学 Q1 CHEMISTRY, ANALYTICAL

Journal of Electroanalytical Chemistry Pub Date : 2025-09-25 DOI:10.1016/j.jelechem.2025.119509

Ziyan You, Rong Chen, Wenfang Deng, Yueming Tan

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引用次数: 0

摘要

由于赭曲霉毒素A （OTA）存在严重的健康风险，为了解决现场检测的关键需求，这项工作开创了一种灵敏的自供电生物传感平台，该平台将非酶DNA行走器与葡萄糖/氧生物燃料电池集成在一起。OTA与其适体的特异性结合触发了两足DNA步行者从步行者-适体双链中释放出来。通过脚点介导的链替换，释放的双足DNA行走器在阳极上的二维DNA轨道上迁移，诱导葡萄糖氧化酶标记的单链DNA探针和DNA轨道之间的杂交。较高的OTA浓度可以改善生物阳极的酶负荷，从而增加生物燃料电池中的短路电流密度，从而实现灵敏的自供电OTA检测。OTA检测的线性范围为10 fg mL−1 ~ 10 ng mL−1，检测限为3 fg mL−1。这项研究开创了一种基于非酶DNA行走器的自供电传感策略，并提供了一种便携式OTA检测设备。

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Sensitive self-powered ochratoxin A detection by coupling a non-enzymatic DNA walker to a glucose/oxygen biofuel cell

Addressing the critical need for on-site ochratoxin A (OTA) detection due to its severe health risks, this work pioneers a sensitive self-powered biosensing platform integrating a non-enzymatic DNA walker with a glucose/oxygen biofuel cell. The specific binding of OTA to its aptamer triggers the release of the bipedal DNA walker from the walker-aptamer duplex. Powered by toehold-mediated strand replacement, the released bipedal DNA walker migrates across the two-dimensional DNA track on the anode, inducing the hybridization between a glucose oxidase-labeled single strand DNA probe and the DNA track. Higher OTA concentrations improve enzymatic loading at the bioanode, leading to increased short-circuit current density in the biofuel cell for sensitive self-powered OTA detection. A linear range from 10 fg mL⁻¹ to 10 ng mL⁻¹ is obtained for OTA detection, with a detection limit of 3 fg mL⁻¹. This study pioneers a non-enzymatic DNA walker-based self-powered sensing strategy and delivers a portable OTA detection device.

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来源期刊

Journal of Electroanalytical Chemistry 化学-电化学

CiteScore

7.80

自引率

6.70%

发文量

912

审稿时长

2.4 months

期刊介绍： The Journal of Electroanalytical Chemistry is the foremost international journal devoted to the interdisciplinary subject of electrochemistry in all its aspects, theoretical as well as applied. Electrochemistry is a wide ranging area that is in a state of continuous evolution. Rather than compiling a long list of topics covered by the Journal, the editors would like to draw particular attention to the key issues of novelty, topicality and quality. Papers should present new and interesting electrochemical science in a way that is accessible to the reader. The presentation and discussion should be at a level that is consistent with the international status of the Journal. Reports describing the application of well-established techniques to problems that are essentially technical will not be accepted. Similarly, papers that report observations but fail to provide adequate interpretation will be rejected by the Editors. Papers dealing with technical electrochemistry should be submitted to other specialist journals unless the authors can show that their work provides substantially new insights into electrochemical processes.