Jinqiao Cao , Shijia Liu , Jiayi Luo , Xia Liang , Quan Wang , Zengjian Liang , Yunshang Ning , Tao Xu , Qiwei Qin , Sumei Xiao , Sheng Zhou
{"title":"口服卵黄抗体对大口黑鲈虹膜病毒具有被动免疫保护作用","authors":"Jinqiao Cao , Shijia Liu , Jiayi Luo , Xia Liang , Quan Wang , Zengjian Liang , Yunshang Ning , Tao Xu , Qiwei Qin , Sumei Xiao , Sheng Zhou","doi":"10.1016/j.fsi.2025.110898","DOIUrl":null,"url":null,"abstract":"<div><div>This study aimed to isolate water-soluble fraction (WSF) containing egg yolk immunoglobulin (IgY) from inactivated Largemouth bass (<em>Micropterus salmoides</em>) iridovirus (LMBV)-immunized egg yolks and assess their oral efficacy in preventing LMBV infection in largemouth bass. Through western blotting and indirect ELISA methods, it was observed that IgY specifically recognized major capsid protein (MCP), with its titer peaking at 8 weeks post-initial immunization and subsequently stabilizing. In vitro, antibody neutralization experiments demonstrated the ability of IgY to neutralize the virus and inhibit its replication. Animal experiments revealed a relative protection rate of approximately 54 % in the specific IgY group, accompanied by significantly reduced viral copy numbers, expression levels of inflammatory factors, and alleviated tissue pathological changes. Serum peptide detection indicated the presence of LMBV and IgY-related peptides circulating in the blood, which may contribute to virus neutralization. These findings suggest that polyclonal antibody IgY exhibits effective preventive properties against LMBV.</div></div>","PeriodicalId":12127,"journal":{"name":"Fish & shellfish immunology","volume":"167 ","pages":"Article 110898"},"PeriodicalIF":3.9000,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Oral feeding of egg yolk antibody provide passive immune protection against largemouth bass iridovirus\",\"authors\":\"Jinqiao Cao , Shijia Liu , Jiayi Luo , Xia Liang , Quan Wang , Zengjian Liang , Yunshang Ning , Tao Xu , Qiwei Qin , Sumei Xiao , Sheng Zhou\",\"doi\":\"10.1016/j.fsi.2025.110898\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>This study aimed to isolate water-soluble fraction (WSF) containing egg yolk immunoglobulin (IgY) from inactivated Largemouth bass (<em>Micropterus salmoides</em>) iridovirus (LMBV)-immunized egg yolks and assess their oral efficacy in preventing LMBV infection in largemouth bass. Through western blotting and indirect ELISA methods, it was observed that IgY specifically recognized major capsid protein (MCP), with its titer peaking at 8 weeks post-initial immunization and subsequently stabilizing. In vitro, antibody neutralization experiments demonstrated the ability of IgY to neutralize the virus and inhibit its replication. Animal experiments revealed a relative protection rate of approximately 54 % in the specific IgY group, accompanied by significantly reduced viral copy numbers, expression levels of inflammatory factors, and alleviated tissue pathological changes. Serum peptide detection indicated the presence of LMBV and IgY-related peptides circulating in the blood, which may contribute to virus neutralization. These findings suggest that polyclonal antibody IgY exhibits effective preventive properties against LMBV.</div></div>\",\"PeriodicalId\":12127,\"journal\":{\"name\":\"Fish & shellfish immunology\",\"volume\":\"167 \",\"pages\":\"Article 110898\"},\"PeriodicalIF\":3.9000,\"publicationDate\":\"2025-09-24\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Fish & shellfish immunology\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1050464825007879\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"FISHERIES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Fish & shellfish immunology","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1050464825007879","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"FISHERIES","Score":null,"Total":0}
Oral feeding of egg yolk antibody provide passive immune protection against largemouth bass iridovirus
This study aimed to isolate water-soluble fraction (WSF) containing egg yolk immunoglobulin (IgY) from inactivated Largemouth bass (Micropterus salmoides) iridovirus (LMBV)-immunized egg yolks and assess their oral efficacy in preventing LMBV infection in largemouth bass. Through western blotting and indirect ELISA methods, it was observed that IgY specifically recognized major capsid protein (MCP), with its titer peaking at 8 weeks post-initial immunization and subsequently stabilizing. In vitro, antibody neutralization experiments demonstrated the ability of IgY to neutralize the virus and inhibit its replication. Animal experiments revealed a relative protection rate of approximately 54 % in the specific IgY group, accompanied by significantly reduced viral copy numbers, expression levels of inflammatory factors, and alleviated tissue pathological changes. Serum peptide detection indicated the presence of LMBV and IgY-related peptides circulating in the blood, which may contribute to virus neutralization. These findings suggest that polyclonal antibody IgY exhibits effective preventive properties against LMBV.
期刊介绍:
Fish and Shellfish Immunology rapidly publishes high-quality, peer-refereed contributions in the expanding fields of fish and shellfish immunology. It presents studies on the basic mechanisms of both the specific and non-specific defense systems, the cells, tissues, and humoral factors involved, their dependence on environmental and intrinsic factors, response to pathogens, response to vaccination, and applied studies on the development of specific vaccines for use in the aquaculture industry.