Lu Li , Yilong Li , Xinyan Chen , Chaoran Lei , Miao Lin , Houyong Luo , Pan Xie , Juan Wei , Guiqiang Fei
{"title":"基于适配体的金属离子和真菌毒素检测生物传感器:原理与进展","authors":"Lu Li , Yilong Li , Xinyan Chen , Chaoran Lei , Miao Lin , Houyong Luo , Pan Xie , Juan Wei , Guiqiang Fei","doi":"10.1016/j.dyepig.2025.113272","DOIUrl":null,"url":null,"abstract":"<div><div>Heavy metal ions and mycotoxins pose serious risks to food safety and public health. Conventional analytical methods such as chromatography and mass spectrometry are accurate but remain costly, labor-intensive, and unsuitable for rapid on-site monitoring. Aptamers, synthetic single-stranded DNA or RNA obtained through SELEX, provide high affinity, good stability, and easy modification, making them attractive recognition elements for biosensor design. This review highlights recent progress in aptamer-based biosensors for detecting toxic metals (Hg<sup>2+</sup>, Pb<sup>2+</sup>, Cd<sup>2+</sup>, As<sup>3+</sup>, Ag<sup>+</sup>, Cu<sup>2+</sup>, Mn<sup>2+</sup>) and mycotoxins (OTA, AFB1, ZEN, DON, FB1). With the aid of nanomaterials, optical and electrochemical transduction, and molecular amplification, many sensors have achieved ultra-low detection limits from nanomolar to femtomolar levels, together with improved selectivity and performance in complex matrices. Current challenges include limited availability of high-affinity aptamers, matrix interference, and lack of standardized protocols. Future advances are expected through high-throughput aptamer screening, multimodal sensing strategies, and integration with portable microfluidic or paper-based devices to promote applications.</div></div>","PeriodicalId":302,"journal":{"name":"Dyes and Pigments","volume":"245 ","pages":"Article 113272"},"PeriodicalIF":4.2000,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Aptamer-based biosensors for metal ion and mycotoxin detection: Principles and progress\",\"authors\":\"Lu Li , Yilong Li , Xinyan Chen , Chaoran Lei , Miao Lin , Houyong Luo , Pan Xie , Juan Wei , Guiqiang Fei\",\"doi\":\"10.1016/j.dyepig.2025.113272\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Heavy metal ions and mycotoxins pose serious risks to food safety and public health. Conventional analytical methods such as chromatography and mass spectrometry are accurate but remain costly, labor-intensive, and unsuitable for rapid on-site monitoring. Aptamers, synthetic single-stranded DNA or RNA obtained through SELEX, provide high affinity, good stability, and easy modification, making them attractive recognition elements for biosensor design. This review highlights recent progress in aptamer-based biosensors for detecting toxic metals (Hg<sup>2+</sup>, Pb<sup>2+</sup>, Cd<sup>2+</sup>, As<sup>3+</sup>, Ag<sup>+</sup>, Cu<sup>2+</sup>, Mn<sup>2+</sup>) and mycotoxins (OTA, AFB1, ZEN, DON, FB1). With the aid of nanomaterials, optical and electrochemical transduction, and molecular amplification, many sensors have achieved ultra-low detection limits from nanomolar to femtomolar levels, together with improved selectivity and performance in complex matrices. Current challenges include limited availability of high-affinity aptamers, matrix interference, and lack of standardized protocols. Future advances are expected through high-throughput aptamer screening, multimodal sensing strategies, and integration with portable microfluidic or paper-based devices to promote applications.</div></div>\",\"PeriodicalId\":302,\"journal\":{\"name\":\"Dyes and Pigments\",\"volume\":\"245 \",\"pages\":\"Article 113272\"},\"PeriodicalIF\":4.2000,\"publicationDate\":\"2025-09-23\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Dyes and Pigments\",\"FirstCategoryId\":\"88\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0143720825006424\",\"RegionNum\":3,\"RegionCategory\":\"工程技术\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"CHEMISTRY, APPLIED\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Dyes and Pigments","FirstCategoryId":"88","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0143720825006424","RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, APPLIED","Score":null,"Total":0}
Aptamer-based biosensors for metal ion and mycotoxin detection: Principles and progress
Heavy metal ions and mycotoxins pose serious risks to food safety and public health. Conventional analytical methods such as chromatography and mass spectrometry are accurate but remain costly, labor-intensive, and unsuitable for rapid on-site monitoring. Aptamers, synthetic single-stranded DNA or RNA obtained through SELEX, provide high affinity, good stability, and easy modification, making them attractive recognition elements for biosensor design. This review highlights recent progress in aptamer-based biosensors for detecting toxic metals (Hg2+, Pb2+, Cd2+, As3+, Ag+, Cu2+, Mn2+) and mycotoxins (OTA, AFB1, ZEN, DON, FB1). With the aid of nanomaterials, optical and electrochemical transduction, and molecular amplification, many sensors have achieved ultra-low detection limits from nanomolar to femtomolar levels, together with improved selectivity and performance in complex matrices. Current challenges include limited availability of high-affinity aptamers, matrix interference, and lack of standardized protocols. Future advances are expected through high-throughput aptamer screening, multimodal sensing strategies, and integration with portable microfluidic or paper-based devices to promote applications.
期刊介绍:
Dyes and Pigments covers the scientific and technical aspects of the chemistry and physics of dyes, pigments and their intermediates. Emphasis is placed on the properties of the colouring matters themselves rather than on their applications or the system in which they may be applied.
Thus the journal accepts research and review papers on the synthesis of dyes, pigments and intermediates, their physical or chemical properties, e.g. spectroscopic, surface, solution or solid state characteristics, the physical aspects of their preparation, e.g. precipitation, nucleation and growth, crystal formation, liquid crystalline characteristics, their photochemical, ecological or biological properties and the relationship between colour and chemical constitution. However, papers are considered which deal with the more fundamental aspects of colourant application and of the interactions of colourants with substrates or media.
The journal will interest a wide variety of workers in a range of disciplines whose work involves dyes, pigments and their intermediates, and provides a platform for investigators with common interests but diverse fields of activity such as cosmetics, reprographics, dye and pigment synthesis, medical research, polymers, etc.