炭黑和聚乙烯微纳塑料对体外血脑屏障模型的调节作用

IF 3.5 3区医学 Q2 FOOD SCIENCE & TECHNOLOGY

Food and Chemical Toxicology Pub Date : 2025-09-24 DOI:10.1016/j.fct.2025.115762

Justina Pajarskienė , Agnė Vailionytė , Ieva Uogintė , Steigvilė Byčenkienė , Agnė Pociūtė , Edvardas Bagdonas , Rūta Aldonytė

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引用次数: 0

摘要

微塑料（MP）和纳米塑料（NP），包括低密度聚乙烯（LDPE），以及碳基颗粒，如炭黑（CBP）和不完全燃烧的黑碳（BC），是广泛存在的环境污染物。它们在空气颗粒物中的共存引起了人们对敏感屏障如血脑屏障（BBB）的潜在影响的担忧。本研究探讨了CBP和LDPE颗粒对体外血脑屏障模型人脑微血管内皮hCMEC/D3细胞的影响。使用稳定的NRF2报告细胞系，我们通过跨内皮电阻（TEER）评估屏障功能，通过共聚焦显微镜评估颗粒摄取，并使用CellProfiler评估形态学变化。炎症和氧化应激反应也被评估。CBP暴露增加了TEER值，表明对屏障完整性有闭塞作用。此外，CBP增强吞噬活性，降低IL-1β水平，表明炎症调节。相比之下，尽管被细胞内化，LDPE颗粒对形态、吞噬、炎症或屏障功能的影响微乎其微。这些发现为研究细胞对空气污染物的反应提供了新的见解，强调了CBP和LDPE颗粒对血脑屏障的不同影响，并强调了对它们的综合影响进行进一步研究的必要性。

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Modulative effects of carbon black and polyethylene micro-nanoplastic particles on blood-brain barrier model in vitro

Microplastics (MP) and nanoplastics (NP), including low-density polyethylene (LDPE), as well as carbon-based particles such as carbon black (CBP) and black carbon (BC) from incomplete combustion, are widespread environmental pollutants. Their co-occurrence in airborne particulate matter raises concerns about potential impacts on sensitive barriers such as the blood-brain barrier (BBB). This study investigates the effects of CBP and LDPE particles on human cerebral microvascular endothelium hCMEC/D3 cells, an in vitro BBB model. Using a stable NRF2 reporter cell line, we assessed barrier function via transendothelial electrical resistance (TEER), particle uptake through confocal microscopy, and morphological changes using CellProfiler. Inflammatory and oxidative stress responses were also evaluated. CBP exposure increased TEER values, suggesting an occluding effect on barrier integrity. Additionally, CBP enhanced phagocytic activity and reduced IL-1β levels, indicating modulation of inflammation. In contrast, although internalized by cells, LDPE particles had minimal impact on morphology, phagocytosis, inflammation, or barrier function. These findings offer new insights into the cellular responses to airborne pollutants, highlighting the differential effects of CBP and LDPE particles on the BBB and underscoring the need for further studies on their combined impact.

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来源期刊

Food and Chemical Toxicology 工程技术-毒理学

CiteScore

10.90

自引率

4.70%

发文量

651

审稿时长

31 days

期刊介绍： Food and Chemical Toxicology (FCT), an internationally renowned journal, that publishes original research articles and reviews on toxic effects, in animals and humans, of natural or synthetic chemicals occurring in the human environment with particular emphasis on food, drugs, and chemicals, including agricultural and industrial safety, and consumer product safety. Areas such as safety evaluation of novel foods and ingredients, biotechnologically-derived products, and nanomaterials are included in the scope of the journal. FCT also encourages submission of papers on inter-relationships between nutrition and toxicology and on in vitro techniques, particularly those fostering the 3 Rs. The principal aim of the journal is to publish high impact, scholarly work and to serve as a multidisciplinary forum for research in toxicology. Papers submitted will be judged on the basis of scientific originality and contribution to the field, quality and subject matter. Studies should address at least one of the following: -Adverse physiological/biochemical, or pathological changes induced by specific defined substances -New techniques for assessing potential toxicity, including molecular biology -Mechanisms underlying toxic phenomena -Toxicological examinations of specific chemicals or consumer products, both those showing adverse effects and those demonstrating safety, that meet current standards of scientific acceptability. Authors must clearly and briefly identify what novel toxic effect (s) or toxic mechanism (s) of the chemical are being reported and what their significance is in the abstract. Furthermore, sufficient doses should be included in order to provide information on NOAEL/LOAEL values.