{"title":"同时检测H2S和黏度的比例近红外荧光探针：在环境监测和生物成像中的应用","authors":"Sha Li, Qin Li, Zhihui Yang, Wei Wen, Miao Yan, Haixian Ren","doi":"10.1016/j.talo.2025.100564","DOIUrl":null,"url":null,"abstract":"<div><div>Hydrogen sulfide (H<sub>2</sub>S), a critical gasotransmitter governing physiological functions, presents significant environmental and food safety hazards at aberrant concentrations. Concurrently, mitochondrial viscosity serves as a vital biomarker for cellular pathologies. To address the unmet need for precise dual-parameter monitoring, we engineered a mitochondria-targeted near-infrared (NIR) ratiometric fluorescent probe, <strong>T-N</strong>, enabling simultaneous and discriminative detection of H<sub>2</sub>S and viscosity through discrete emission channels. The probe exhibits ultrarapid response (<30 s), exceptional sensitivity, and superb selectivity against competing analytes. Leveraging H<sub>2</sub>S-triggered chromogenic shifts, <strong>T-N</strong>-immobilized test strips facilitate instrument-free, semi-quantitative assessment of food spoilage, validated across environmental waters and industrially relevant beer samples. Moreover, <strong>T-N</strong> achieves synchronous dual-channel imaging of endogenous/exogenous H<sub>2</sub>S and viscosity dynamics in live cells, with verified mitochondrial localization. This work establishes a versatile sensing platform for tracking H<sub>2</sub>S and viscosity in interconnected environmental, food safety, and biomedical contexts.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"12 ","pages":"Article 100564"},"PeriodicalIF":3.7000,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"A ratiometric NIR fluorescent probe for simultaneous detection of H2S and viscosity: Applications in environmental monitoring and bioimaging\",\"authors\":\"Sha Li, Qin Li, Zhihui Yang, Wei Wen, Miao Yan, Haixian Ren\",\"doi\":\"10.1016/j.talo.2025.100564\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Hydrogen sulfide (H<sub>2</sub>S), a critical gasotransmitter governing physiological functions, presents significant environmental and food safety hazards at aberrant concentrations. Concurrently, mitochondrial viscosity serves as a vital biomarker for cellular pathologies. To address the unmet need for precise dual-parameter monitoring, we engineered a mitochondria-targeted near-infrared (NIR) ratiometric fluorescent probe, <strong>T-N</strong>, enabling simultaneous and discriminative detection of H<sub>2</sub>S and viscosity through discrete emission channels. The probe exhibits ultrarapid response (<30 s), exceptional sensitivity, and superb selectivity against competing analytes. Leveraging H<sub>2</sub>S-triggered chromogenic shifts, <strong>T-N</strong>-immobilized test strips facilitate instrument-free, semi-quantitative assessment of food spoilage, validated across environmental waters and industrially relevant beer samples. Moreover, <strong>T-N</strong> achieves synchronous dual-channel imaging of endogenous/exogenous H<sub>2</sub>S and viscosity dynamics in live cells, with verified mitochondrial localization. This work establishes a versatile sensing platform for tracking H<sub>2</sub>S and viscosity in interconnected environmental, food safety, and biomedical contexts.</div></div>\",\"PeriodicalId\":436,\"journal\":{\"name\":\"Talanta Open\",\"volume\":\"12 \",\"pages\":\"Article 100564\"},\"PeriodicalIF\":3.7000,\"publicationDate\":\"2025-09-23\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Talanta Open\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2666831925001651\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"CHEMISTRY, ANALYTICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Talanta Open","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2666831925001651","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
A ratiometric NIR fluorescent probe for simultaneous detection of H2S and viscosity: Applications in environmental monitoring and bioimaging
Hydrogen sulfide (H2S), a critical gasotransmitter governing physiological functions, presents significant environmental and food safety hazards at aberrant concentrations. Concurrently, mitochondrial viscosity serves as a vital biomarker for cellular pathologies. To address the unmet need for precise dual-parameter monitoring, we engineered a mitochondria-targeted near-infrared (NIR) ratiometric fluorescent probe, T-N, enabling simultaneous and discriminative detection of H2S and viscosity through discrete emission channels. The probe exhibits ultrarapid response (<30 s), exceptional sensitivity, and superb selectivity against competing analytes. Leveraging H2S-triggered chromogenic shifts, T-N-immobilized test strips facilitate instrument-free, semi-quantitative assessment of food spoilage, validated across environmental waters and industrially relevant beer samples. Moreover, T-N achieves synchronous dual-channel imaging of endogenous/exogenous H2S and viscosity dynamics in live cells, with verified mitochondrial localization. This work establishes a versatile sensing platform for tracking H2S and viscosity in interconnected environmental, food safety, and biomedical contexts.
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