用DNA条形码设计纳米颗粒表面用于体内精确定量。

IF 8.7 Q1 CHEMISTRY, MULTIDISCIPLINARY
JACS Au Pub Date : 2025-08-25 eCollection Date: 2025-09-22 DOI:10.1021/jacsau.5c00475
Ayokunle A Lekuti, Vanessa Y C Li, Ayden Malekjahani, Sara Ahmed, Stefan M Mladjenovic, Marshall G G Macduff, Warren C W Chan
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引用次数: 0

摘要

DNA条形码是鉴定纳米颗粒生物分布的常用方法。DNA条形码通常封装在纳米颗粒中,以确保下一代测序的准确测量。这种方法限制了可以筛选的纳米颗粒的类型。DNA也可以被包裹在纳米颗粒表面。然而,目前尚不清楚表面包裹的DNA是否可以用作条形码,因为它们可以降解,这使得纳米颗粒设计的鉴定和定量具有挑战性。在这里,我们开发了减少纳米颗粒表面DNA降解的策略,允许基于表面的DNA条形码用于生物分布应用。我们证明纳米颗粒大小、DNA密度、聚合物长度和密度是准确识别和定量体内纳米颗粒的基本设计参数。我们发现DNA的化学修饰和使用中性聚合物的屏蔽可以减少DNA的降解。我们验证了表面条形码可以确定纳米颗粒在体内的分布。我们的发现为使用基于表面的DNA条形码在体内筛选靶向应用的纳米颗粒配方铺平了道路。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Designing Nanoparticle Surfaces with DNA Barcodes for Accurate In Vivo Quantification.

Designing Nanoparticle Surfaces with DNA Barcodes for Accurate In Vivo Quantification.

Designing Nanoparticle Surfaces with DNA Barcodes for Accurate In Vivo Quantification.

Designing Nanoparticle Surfaces with DNA Barcodes for Accurate In Vivo Quantification.

DNA barcoding is a common method for identifying the biodistribution of nanoparticles. DNA barcodes are typically encapsulated within nanoparticles to ensure accurate measurements by next-generation sequencing. This method limits the types of nanoparticles that can be screened. DNA can also be coated on nanoparticle surfaces. However, it is unclear whether surface-coated DNA can be used as barcodes because they can degrade, making the identification and quantification of nanoparticle designs challenging. Here, we developed strategies to reduce DNA degradation on nanoparticle surfaces, allowing surface-based DNA barcodes for biodistribution applications. We demonstrate that nanoparticle size, DNA density, and polymer length and density are essential design parameters for accurately identifying and quantifying nanoparticles in vivo. We found that chemical modification of DNA and shielding using neutral polymers reduce DNA degradation. We validated that surface barcoding can determine the in vivo distribution of nanoparticles. Our findings pave the way for the use of surface-based DNA barcodes for in vivo screening of nanoparticle formulations for targeted applications.

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来源期刊
CiteScore
9.10
自引率
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