Hanan Hassan Sabry, Zainab Sami Noor, Rasha Abdel Hameed Elsayed, Nader Nasr Nazmy
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Clinical data, including Psoriasis Area and Severity Index (PASI) scores, were collected. Skin microbiome analysis was performed using culture methods, serum IL-23 levels were measured by ELISA, and genetic analysis of the IL-23 rs2201841 polymorphism was conducted using PCR-RFLP.</p><p><strong>Results: </strong>Psoriasis patients exhibited higher fungal growth (75% vs. 17.5%, <i>p</i> < 0.001) and mixed bacterial-fungal infections (55% vs. 15%, <i>p</i> < 0.001). Serum IL-23 levels were significantly lower in patients (157.2 ±214.8 pg/ml vs. 252.7 ±308.2 pg/ml, <i>p</i> < 0.001) and negatively correlated with PASI scores (<i>r</i> = -0.866, <i>p</i> < 0.001). The IL-23 rs2201841 SNP showed no significant association with psoriasis. Fungal growth (OR = 3.61, <i>p</i> = 0.002) and reduced IL-23 levels (OR = 0.996, <i>p</i> = 0.039) independently predicted disease susceptibility.</p><p><strong>Conclusions: </strong>Alterations in the skin microbiome and reduced IL-23 levels are associated with psoriasis. These findings suggest fungal infections and IL-23 as potential biomarkers for disease diagnosis and monitoring.</p>","PeriodicalId":54595,"journal":{"name":"Postepy Dermatologii I Alergologii","volume":"42 4","pages":"361-372"},"PeriodicalIF":1.4000,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12458058/pdf/","citationCount":"0","resultStr":"{\"title\":\"Association of skin microbiome with interleukin-23 in patients with plaque psoriasis.\",\"authors\":\"Hanan Hassan Sabry, Zainab Sami Noor, Rasha Abdel Hameed Elsayed, Nader Nasr Nazmy\",\"doi\":\"10.5114/ada.2025.153336\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Introduction: </strong>Psoriasis is a chronic autoimmune disease influenced by immune dysregulation and microbiome alterations. The IL-23/Th17 axis is central to its pathogenesis, promoting inflammation and keratinocyte hyperproliferation. Skin microbiome dysbiosis has been implicated in disease onset, but its relationship with IL-23 requires further exploration.</p><p><strong>Aim: </strong>This study aimed to investigate variations in the skin microbiome between psoriatic patients and healthy controls, and examine the correlation of IL-23 serum levels and genetic polymorphisms with psoriasis presence and severity.</p><p><strong>Material and methods: </strong>This case-control study included 40 patients with plaque psoriasis and 40 healthy controls. Clinical data, including Psoriasis Area and Severity Index (PASI) scores, were collected. Skin microbiome analysis was performed using culture methods, serum IL-23 levels were measured by ELISA, and genetic analysis of the IL-23 rs2201841 polymorphism was conducted using PCR-RFLP.</p><p><strong>Results: </strong>Psoriasis patients exhibited higher fungal growth (75% vs. 17.5%, <i>p</i> < 0.001) and mixed bacterial-fungal infections (55% vs. 15%, <i>p</i> < 0.001). Serum IL-23 levels were significantly lower in patients (157.2 ±214.8 pg/ml vs. 252.7 ±308.2 pg/ml, <i>p</i> < 0.001) and negatively correlated with PASI scores (<i>r</i> = -0.866, <i>p</i> < 0.001). The IL-23 rs2201841 SNP showed no significant association with psoriasis. Fungal growth (OR = 3.61, <i>p</i> = 0.002) and reduced IL-23 levels (OR = 0.996, <i>p</i> = 0.039) independently predicted disease susceptibility.</p><p><strong>Conclusions: </strong>Alterations in the skin microbiome and reduced IL-23 levels are associated with psoriasis. 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引用次数: 0
摘要
银屑病是一种由免疫失调和微生物群改变影响的慢性自身免疫性疾病。IL-23/Th17轴是其发病机制的核心,促进炎症和角化细胞过度增殖。皮肤微生物群失调与疾病发病有关,但其与IL-23的关系有待进一步探讨。目的:本研究旨在探讨银屑病患者与健康对照者皮肤微生物组的差异,并研究IL-23血清水平和基因多态性与银屑病存在和严重程度的相关性。材料和方法:本病例对照研究包括40例斑块型银屑病患者和40例健康对照者。收集临床数据,包括银屑病面积和严重程度指数(PASI)评分。采用培养法分析皮肤微生物组,ELISA法检测血清IL-23水平,PCR-RFLP法分析IL-23 rs2201841多态性。结果:银屑病患者真菌生长(75% vs. 17.5%, p < 0.001)和细菌-真菌混合感染(55% vs. 15%, p < 0.001)较高。患者血清IL-23水平显著降低(157.2±214.8 pg/ml vs. 252.7±308.2 pg/ml, p < 0.001),且与PASI评分呈负相关(r = -0.866, p < 0.001)。IL-23 rs2201841 SNP与银屑病无显著相关性。真菌生长(OR = 3.61, p = 0.002)和IL-23水平降低(OR = 0.996, p = 0.039)独立预测疾病易感性。结论:皮肤微生物组的改变和IL-23水平的降低与牛皮癣有关。这些发现提示真菌感染和IL-23是疾病诊断和监测的潜在生物标志物。
Association of skin microbiome with interleukin-23 in patients with plaque psoriasis.
Introduction: Psoriasis is a chronic autoimmune disease influenced by immune dysregulation and microbiome alterations. The IL-23/Th17 axis is central to its pathogenesis, promoting inflammation and keratinocyte hyperproliferation. Skin microbiome dysbiosis has been implicated in disease onset, but its relationship with IL-23 requires further exploration.
Aim: This study aimed to investigate variations in the skin microbiome between psoriatic patients and healthy controls, and examine the correlation of IL-23 serum levels and genetic polymorphisms with psoriasis presence and severity.
Material and methods: This case-control study included 40 patients with plaque psoriasis and 40 healthy controls. Clinical data, including Psoriasis Area and Severity Index (PASI) scores, were collected. Skin microbiome analysis was performed using culture methods, serum IL-23 levels were measured by ELISA, and genetic analysis of the IL-23 rs2201841 polymorphism was conducted using PCR-RFLP.
Results: Psoriasis patients exhibited higher fungal growth (75% vs. 17.5%, p < 0.001) and mixed bacterial-fungal infections (55% vs. 15%, p < 0.001). Serum IL-23 levels were significantly lower in patients (157.2 ±214.8 pg/ml vs. 252.7 ±308.2 pg/ml, p < 0.001) and negatively correlated with PASI scores (r = -0.866, p < 0.001). The IL-23 rs2201841 SNP showed no significant association with psoriasis. Fungal growth (OR = 3.61, p = 0.002) and reduced IL-23 levels (OR = 0.996, p = 0.039) independently predicted disease susceptibility.
Conclusions: Alterations in the skin microbiome and reduced IL-23 levels are associated with psoriasis. These findings suggest fungal infections and IL-23 as potential biomarkers for disease diagnosis and monitoring.