暴露于低剂量聚苯乙烯纳米塑料通过减少发情周期来损害。

IF 2.8 4区 医学 Q2 REPRODUCTIVE BIOLOGY
Lethícia Valencise , Jorge Willian Franco de Barros , Ana Flávia Quiarato Lozano , Luan Reis Calixto , Daniel G. Cyr , Wilma De Grava Kempinas
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引用次数: 0

摘要

塑料可以被分解成更小的碎片,称为微塑料(小于5毫米)或纳米塑料(小于1微米)。据报道,这些颗粒可以跨越生物屏障,并在几种组织类型中引起氧化应激损伤。鉴于雌性生殖组织被认为是这些颗粒的目标,我们的研究旨在评估低浓度(0.015mg/d)聚苯乙烯纳米塑料(PS-NP, 500nm)对成年雌性Wistar大鼠生殖参数的影响。以0.015mg/d的蒸馏水稀释PS-NP灌胃25 d,每组10只。对照组仅给予蒸馏水(载水)。我们评估了体重增加、发情周期、性行为和生育能力、卵巢和子宫形态、卵巢StAR免疫染色和血清类固醇激素水平:雌二醇和黄体酮。采用学生t检验、Mann-Whitney检验或Fisher精确检验对数据进行评估。当P≤0.05时,认为结果有显著差异。在实验条件下,PS-NP组出现动情周期失调,子宫炎症浸润,子宫和垂体重量增加,甲状腺重量下降。这些发现可能是由于黄体细胞中StAR表达减少,从而导致黄体酮血清水平降低。这些结果表明,在啮齿动物模型中,纳米塑料作为内分泌干扰物,损害了雌性内分泌和生殖功能,并引起了对其他雌性和成年女性接触纳米塑料后的结果的关注。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Exposure to low-dose polystyrene nanoplastics impairs the estrous cycle by decreasing ovarian levels of steroidogenic acute regulatory protein and serum progesterone levels in rats
Plastic can be fragmented into smaller pieces referred to as microplastics (< 5 mm), or nanoplastics (< 1 µm). These particles have been reported to cross biological barriers and cause oxidative stress damage in several tissue types. Given that female reproductive tissues are considered a target for such particles, our study aimed to evaluate the effects of polystyrene nanoplastics (PS-NP, 500 nm) at a low concentration (0.015 mg/d), on reproductive parameters of adult female Wistar rats. Animals (n = 10/group) were treated by gavage for 25 days with PS-NP diluted in distilled water at a concentration of 0.015 mg/d. The Control group received only distilled water (vehicle). We assessed weight gain, estrous cyclicity, sexual behavior and fertility, morphology of ovaries and uteri, immunostaining for StAR in the ovaries, and serum levels of the steroid hormones: estradiol and progesterone. Data was evaluated by Student’s t-test, Mann-Whitney test, or Fisher's Exact test. Results were considered significantly different when P ≤ 0.05. The PS-NP group showed estrous cycle dysregulation, uterine inflammatory infiltration, increased uterus and pituitary weight, and decreased thyroid weight in the experimental conditions utilized. These findings are potentially due to the decrease in StAR expression in luteal cells, and consequent reduction of progesterone serum levels. These results indicate that nanoplastics act as endocrine disruptors impairing female endocrine and reproductive function, in a rodent model, and raise concern about outcomes after exposure to nanoplastics in other females and in adult women's reproductive health.
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来源期刊
Reproductive toxicology
Reproductive toxicology 生物-毒理学
CiteScore
6.50
自引率
3.00%
发文量
131
审稿时长
45 days
期刊介绍: Drawing from a large number of disciplines, Reproductive Toxicology publishes timely, original research on the influence of chemical and physical agents on reproduction. Written by and for obstetricians, pediatricians, embryologists, teratologists, geneticists, toxicologists, andrologists, and others interested in detecting potential reproductive hazards, the journal is a forum for communication among researchers and practitioners. Articles focus on the application of in vitro, animal and clinical research to the practice of clinical medicine. All aspects of reproduction are within the scope of Reproductive Toxicology, including the formation and maturation of male and female gametes, sexual function, the events surrounding the fusion of gametes and the development of the fertilized ovum, nourishment and transport of the conceptus within the genital tract, implantation, embryogenesis, intrauterine growth, placentation and placental function, parturition, lactation and neonatal survival. Adverse reproductive effects in males will be considered as significant as adverse effects occurring in females. To provide a balanced presentation of approaches, equal emphasis will be given to clinical and animal or in vitro work. Typical end points that will be studied by contributors include infertility, sexual dysfunction, spontaneous abortion, malformations, abnormal histogenesis, stillbirth, intrauterine growth retardation, prematurity, behavioral abnormalities, and perinatal mortality.
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