蛋白质组学分析推断保护细胞线粒体中优化的atp生产。

IF 3.6 2区 生物学 Q1 PLANT SCIENCES
Noah Ditz, Markus Niehaus, Nieves Medina Escobar, Marco Herde, Holger Eubel
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引用次数: 0

摘要

保卫细胞是高度特化的叶片细胞,它调节叶片的蒸腾作用和固碳作用。因此,它们在平衡水分利用效率和光合作用方面起着重要作用。保护细胞的这种独特功能要求它们快速积累溶质,用于依赖atp的气孔打开,但这如何影响线粒体能量代谢仍然是难以捉摸的。利用细胞类型特异性亲和纯化拟南芥保护细胞和叶肉细胞线粒体,我们旨在揭示保护细胞线粒体的酶结构,以便对这些特殊细胞器的代谢特性有一个初步的了解。与叶肉细胞相比,使用替代底物生产NADH,远离非质子泵吸呼吸酶,以及较低的NADH再氧化率表明,保护细胞线粒体中有一种专门的ATP生产模式。此外,线粒体进口机制的丰度降低也间接意味着这些细胞器中的蛋白质周转率降低。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Proteomic Analysis Infers Optimized ATP-Production in Guard Cell Mitochondria.

Guard cells are highly specialised leaf cells which regulate leaf transpiration and carbon fixation. As such, they are instrumental in balancing water use efficiency and photosynthetic activity. This unique function of guard cells requires them to quickly accumulate solutes for ATP-dependent stomatal opening, but how this affects mitochondrial energy metabolism remains elusive. Using cell-type-specific affinity purification of Arabidopsis thaliana guard cell and mesophyll cell mitochondria, we aim at unravelling the enzymatic configuration of guard cell mitochondria in order to provide a first glance at the metabolic properties of these specialised organelles. When compared to their mesophyll cell counterparts, the use of alternative substrates for NADH production, a shift away from non-proton pumping respiratory enzymes, and a lower NADH re-oxidation rate suggest a specialised mode of ATP production in guard cell mitochondria. In addition, a lowered abundance of the mitochondrial import machinery also indirectly implies lower protein turnover rates in these organelles.

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来源期刊
Physiologia plantarum
Physiologia plantarum 生物-植物科学
CiteScore
11.00
自引率
3.10%
发文量
224
审稿时长
3.9 months
期刊介绍: Physiologia Plantarum is an international journal committed to publishing the best full-length original research papers that advance our understanding of primary mechanisms of plant development, growth and productivity as well as plant interactions with the biotic and abiotic environment. All organisational levels of experimental plant biology – from molecular and cell biology, biochemistry and biophysics to ecophysiology and global change biology – fall within the scope of the journal. The content is distributed between 5 main subject areas supervised by Subject Editors specialised in the respective domain: (1) biochemistry and metabolism, (2) ecophysiology, stress and adaptation, (3) uptake, transport and assimilation, (4) development, growth and differentiation, (5) photobiology and photosynthesis.
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