Noah Ditz, Markus Niehaus, Nieves Medina Escobar, Marco Herde, Holger Eubel
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Proteomic Analysis Infers Optimized ATP-Production in Guard Cell Mitochondria.
Guard cells are highly specialised leaf cells which regulate leaf transpiration and carbon fixation. As such, they are instrumental in balancing water use efficiency and photosynthetic activity. This unique function of guard cells requires them to quickly accumulate solutes for ATP-dependent stomatal opening, but how this affects mitochondrial energy metabolism remains elusive. Using cell-type-specific affinity purification of Arabidopsis thaliana guard cell and mesophyll cell mitochondria, we aim at unravelling the enzymatic configuration of guard cell mitochondria in order to provide a first glance at the metabolic properties of these specialised organelles. When compared to their mesophyll cell counterparts, the use of alternative substrates for NADH production, a shift away from non-proton pumping respiratory enzymes, and a lower NADH re-oxidation rate suggest a specialised mode of ATP production in guard cell mitochondria. In addition, a lowered abundance of the mitochondrial import machinery also indirectly implies lower protein turnover rates in these organelles.
期刊介绍:
Physiologia Plantarum is an international journal committed to publishing the best full-length original research papers that advance our understanding of primary mechanisms of plant development, growth and productivity as well as plant interactions with the biotic and abiotic environment. All organisational levels of experimental plant biology – from molecular and cell biology, biochemistry and biophysics to ecophysiology and global change biology – fall within the scope of the journal. The content is distributed between 5 main subject areas supervised by Subject Editors specialised in the respective domain: (1) biochemistry and metabolism, (2) ecophysiology, stress and adaptation, (3) uptake, transport and assimilation, (4) development, growth and differentiation, (5) photobiology and photosynthesis.