羧基小体的功能、进化和未来。

IF 5.7 2区 生物学 Q1 PLANT SCIENCES
Nghiem D Nguyen, Loraine M Rourke, G Dean Price, Benedict M Long
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引用次数: 0

摘要

自从50多年前它们被发现为含有红宝石的隔室以来,重大突破已经推进了我们对羧基体结构和功能的理解,以及它们在蓝藻二氧化碳浓缩机制(CCMs)中的中心地位。在CCMs中,细胞碳酸氢盐(HCO3-)被一系列HCO3-转运体和二氧化碳到HCO3-转化泵积极积累和维持,以支持羧酸体的功能。细胞中HCO3-的升高对于羧酸体的作用是必不可少的,因为它们完全依赖于浓缩的HCO3-来产生rubisco内部的CO2。本文回顾了羧基体研究的历史进展,从早期的结构观察到现代对其生物发生、内部组织和功能的认识。我们探索羧酸体的进化轨迹,假设为什么陆生植物,尽管与蓝藻有共同的祖先,却缺乏这些微室。尽管在植物中没有羧酸体,但作为提高光合作用性能的努力的一部分,它们现在被改造成植物叶绿体。我们还讨论了羧基体气体渗透性的生理意义,氧合的作用,以及在工程系统中评估羧基体功能的体外分析的必要性。我们讨论了在异源系统中重建功能羧基体的挑战,特别是对HCO3-积累的需要。最后,我们考虑了羧基体的未来,包括它们作为碳固定和新型催化功能的模块化平台及其潜在的向前进化轨迹。通过综合历史、机制和应用方面的观点,本文综述了rubisco在异源系统中包封的可能性和局限性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The Function, Evolution, and Future of Carboxysomes.

Since their discovery as rubisco-containing compartments more than 50 years ago, significant breakthroughs have advanced our understanding of carboxysome structure and function, and their centrality to cyanobacterial CO2 concentrating mechanisms (CCMs). Within CCMs, cellular bicarbonate (HCO3-) is actively accumulated and maintained by a suite of HCO3- transporters and CO2-to-HCO3- conversion pumps to support carboxysome function. This elevation of cellular HCO3- is indispensable for carboxysome action, as they rely entirely on concentrated HCO3- to generate internal CO2 for rubisco. This review traces the historical progression of carboxysome research, from early structural observations to modern insights into their biogenesis, internal organization, and function. We explore the evolutionary trajectory of carboxysomes, hypothesising why terrestrial plants, despite sharing a common ancestor with cyanobacteria, lack these microcompartments. Despite their absence from plants, carboxysomes are now being engineered into plant chloroplasts as part of efforts to improve photosynthetic performance. We also address the physiological implications of carboxysome gas permeability, the role of oxygenation, and the need for in vitro assays to assess carboxysome functionality in engineered systems. We discuss the challenges of reconstructing functional carboxysomes in heterologous systems, particularly the need for HCO3- accumulation. Finally, we consider the future of carboxysomes, including their use as modular platforms for carbon fixation and novel catalytic functions and their potential forward evolutionary trajectories. By synthesizing historical, mechanistic, and applied perspectives, this review highlights both the possibilities and limitations of rubisco encapsulation in heterologous systems.

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来源期刊
Journal of Experimental Botany
Journal of Experimental Botany 生物-植物科学
CiteScore
12.30
自引率
4.30%
发文量
450
审稿时长
1.9 months
期刊介绍: The Journal of Experimental Botany publishes high-quality primary research and review papers in the plant sciences. These papers cover a range of disciplines from molecular and cellular physiology and biochemistry through whole plant physiology to community physiology. Full-length primary papers should contribute to our understanding of how plants develop and function, and should provide new insights into biological processes. The journal will not publish purely descriptive papers or papers that report a well-known process in a species in which the process has not been identified previously. Articles should be concise and generally limited to 10 printed pages.
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