在口腔崩解膜中注入蛋白质和肽以预防体内早期牙蚀的口腔护理新前沿。

IF 5.5 2区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of dentistry Pub Date : 2025-09-23 DOI:10.1016/j.jdent.2025.106129

Vinícius Taioqui Pelá , Gustavo Cassalate da Silva , Mariana Miranda de Brito , Even Akemi Taira , Carolina Ruis Ferrari , Talita Mendes Oliveira Ventura , Larissa Tercilia Grizzo Thomassian , Flávio Henrique-Silva , Eduardo Pereira de Souza , Thiago Saads Carvalho , Andrea Soares da Costa Fuentes , Karina Fan , Reinaldo Marchetto , Adrian Lussi , Marília Afonso Rabelo Buzalaf

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引用次数: 0

摘要

目的：研究不同蛋白和肽配制的口腔崩解膜（ODFs）在体内减少早期牙侵蚀的效果。方法：12人参加6期交叉试验：安慰剂、1mg氟化钠（NaF）、1mg甘蔗衍生胱抑素（CaneCPI-5）、5mg猕猴桃衍生胱抑素（MaquiCPI-3）、1mg橙子衍生胱抑素（CsinCPI-2）和0.37mg胱抑素肽（stn15sps）。在预防后，参与者将odf放在舌头上，直到它们溶解，在获得的膜形成2小时后。在上颌中门牙的唇表面进行侵蚀挑战（活检，1%柠檬酸，15秒）。使用相对表面反射强度（%SRI）评估牙釉质损失，使用偶氮胂iii测量柠檬酸中的钙释放。使用Kruskal-Wallis和Dunn的事后测试对数据进行分析(结果：%SRI结果表明，与安慰剂相比，MaquiCPI-3、CaneCPI-5、stn15psp和NaF提供了更大的牙釉质保护(结论：根据%SRI，含有MaquiCPI-3、CaneCPI-5和stn15psp的odf有效地抑制了早期牙侵蚀。临床意义：这些发现强调了odf作为有机化合物的创新递送系统的潜力，旨在防止牙齿侵蚀磨损和促进口腔健康。

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A new frontier in oral care with proteins and peptide infused in orally disintegrating film to prevent early dental erosion in vivo

Objective

This study investigated the effectiveness of orally disintegrating films (ODFs) formulated with different proteins and peptide in minimizing early-stage dental erosion in vivo.

Methods

Twelve individuals participated in a six-phase crossover: placebo, 1 mg sodium fluoride (NaF), 1 mg sugarcane-derived cystatin (CaneCPI-5), 5 mg maquiberry-derived cystatin (MaquiCPI-3), 1 mg orange-derived cystatin (CsinCPI-2), and 0.37 mg statherin peptide (StN15pSpS). Following prophylaxis, participants placed the ODFs on their tongues until they dissolved, after the acquired pellicle was allowed to form over 2 h. An erosive challenge (biopsy, 1 % citric acid, 15 s) was conducted on the labial surface of the maxillary central incisors. Enamel loss was assessed using Relative Surface Reflection Intensity (%SRI), and calcium release in citric acid was measured using Arsenazo-III. Data were analyzed using Kruskal-Wallis and Dunn’s post hoc tests (p < 0.05). A questionnaire assessed participants’ satisfaction and any side effects.

Results

The %SRI results demonstrated that MaquiCPI-3, CaneCPI-5, StN15pSpS, and NaF provided significantly greater enamel protection compared to the placebo (p < 0.05). Calcium analysis showed that the ODFs containing CaneCPI-5 and MaquiCPI-3 resulted in similarly high calcium levels, whereas the placebo and NaF groups exhibited the lowest values, with no difference between them. The CsinCPI-2 and StN15pSpS groups displayed intermediate effects, with no significant differences compared to the other groups, except for CsinCPI-2, which presented significantly reduced calcium levels compared to the MaquiCPI-3 group (p < 0.05). Participants reported satisfaction with the taste of the ODFs, found the procedures acceptable, and noted no adverse effects.

Conclusions

ODFs containing MaquiCPI-3, CaneCPI-5, and StN15pSpS effectively inhibited early dental erosion according to %SRI.

Clinical Significance

These findings highlight the potential of ODFs as innovative delivery systems for organic compounds aimed at preventing erosive tooth wear and promoting oral health.

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来源期刊

Journal of dentistry 医学-牙科与口腔外科

CiteScore

7.30

自引率

11.40%

发文量

349

审稿时长

35 days

期刊介绍： The Journal of Dentistry has an open access mirror journal The Journal of Dentistry: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review. The Journal of Dentistry is the leading international dental journal within the field of Restorative Dentistry. Placing an emphasis on publishing novel and high-quality research papers, the Journal aims to influence the practice of dentistry at clinician, research, industry and policy-maker level on an international basis. Topics covered include the management of dental disease, periodontology, endodontology, operative dentistry, fixed and removable prosthodontics, dental biomaterials science, long-term clinical trials including epidemiology and oral health, technology transfer of new scientific instrumentation or procedures, as well as clinically relevant oral biology and translational research. The Journal of Dentistry will publish original scientific research papers including short communications. It is also interested in publishing review articles and leaders in themed areas which will be linked to new scientific research. Conference proceedings are also welcome and expressions of interest should be communicated to the Editor.