发育神经元中肌动蛋白波和生长锥导航的纳米形貌控制。

IF 4.6 2区 生物学 Q2 CELL BIOLOGY
Frontiers in Cell and Developmental Biology Pub Date : 2025-09-10 eCollection Date: 2025-01-01 DOI:10.3389/fcell.2025.1631520
Spandan Pathak, Kate M O'Neill, Emily K Robinson, Matt J Hourwitz, Corey Herr, John T Fourkas, Edward Giniger, Wolfgang Losert
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引用次数: 0

摘要

神经回路中轴突和树突(神经突)的发育依赖于细胞骨架成分(尤其是肌动蛋白)的动态相互作用,以及各种环境信号的整合。基于先前的发现,肌动蛋白动力学可以作为物理引导线索的主要传感器,本研究探讨了纳米形貌在早期神经回路发育过程中调节和引导肌动蛋白波和神经突尖端动力学中的作用。虽然众所周知,肌动蛋白动力学有助于宽轴突尖端(通常称为生长锥)的寻路,但我们也观察到整个神经突和其他较窄的神经突尖端的动态肌动蛋白重塑。我们发现,在平面基底上的神经突发育的前两周内,肌动蛋白波速度没有显著变化,但在纳米脊上的神经突发育的同一时期内,肌动蛋白波速度会下降。随着神经突的成熟,纳米脊引导肌动蛋白波和神经突尖端方向的能力也在下降,无论是窄尖端还是宽生长锥。随着神经元成熟,对物理引导信号的反应发生变化,可能会影响植入成熟大脑的发育中的神经细胞的再生能力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Nanotopographic control of actin waves and growth cone navigation in developing neurons.

The development of axons and dendrites (neurites) in a neural circuit relies on the dynamic interplay of cytoskeletal components, especially actin, and the integration of diverse environmental cues. Building on prior findings that actin dynamics can serve as a primary sensor of physical guidance cues, this work investigates the role of nanotopography in modulating and guiding actin waves and neurite-tip dynamics during early neural circuit development. Although actin dynamics is well known to contribute to pathfinding in wide axonal tips, typically referred to as growth cones, we also observe dynamic actin remodeling throughout neurites and at other, narrower, neurite tips. We find that actin-wave speeds do not change significantly in the first 2 weeks of neurite development on flat substrates, but decrease over the same period in neurites on nanoridges. The ability of nanoridges to guide actin waves and the neurite-tip direction also decreases as neurites mature, both for narrow tips and wide growth cones. This change in responsiveness to physical guidance cues with neuronal maturation may impact the regenerative capacity of developing neural cells that are inserted into mature brains.

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来源期刊
Frontiers in Cell and Developmental Biology
Frontiers in Cell and Developmental Biology Biochemistry, Genetics and Molecular Biology-Cell Biology
CiteScore
9.70
自引率
3.60%
发文量
2531
审稿时长
12 weeks
期刊介绍: Frontiers in Cell and Developmental Biology is a broad-scope, interdisciplinary open-access journal, focusing on the fundamental processes of life, led by Prof Amanda Fisher and supported by a geographically diverse, high-quality editorial board. The journal welcomes submissions on a wide spectrum of cell and developmental biology, covering intracellular and extracellular dynamics, with sections focusing on signaling, adhesion, migration, cell death and survival and membrane trafficking. Additionally, the journal offers sections dedicated to the cutting edge of fundamental and translational research in molecular medicine and stem cell biology. With a collaborative, rigorous and transparent peer-review, the journal produces the highest scientific quality in both fundamental and applied research, and advanced article level metrics measure the real-time impact and influence of each publication.
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