利用浓缩肉汤优化检测直肠拭子中ESBL产生物、碳青霉烯酶产生物和VRE，具有较高的筛选敏感性。

IF 3 3区医学 Q2 INFECTIOUS DISEASES

European Journal of Clinical Microbiology & Infectious Diseases Pub Date : 2025-09-26 DOI:10.1007/s10096-025-05288-1

Dominique S Blanc, Massiha Kamyar, Patrice Nordmann, Laurent Poirel, Gilbert Greub

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引用次数: 0

摘要

本研究评估了使用浓缩菌液提高直肠棉签中多药耐药（MDR）细菌——vre、ESBL产生菌和碳青霉烯酶产生菌（CPOs）检测的附加值。将直接镀法与富集法进行比较，可显著提高检测灵敏度，特别是对CPOs（从17例增加到27例，增加58.8%）。ESBL生产者和VRE的检出率也显著提高（提高了20%）。因此，预富集是对常规耐多药细菌筛选方案的宝贵补充，在预防和感染控制以及疫情管理需要高精度时特别有用。

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Optimized detection of ESBL producers, carbapenemase producers, and VRE from rectal swabs using enrichment broth for superior screening sensitivity.

This study evaluates the added value of using enrichment broths for improving the detection of multidrug-resistant (MDR) bacteria-VRE, ESBL producers, and carbapenemase-producing organisms (CPOs)-from rectal swabs. Comparing direct plating to enrichment-based methods significantly increased detection sensitivity, especially for CPOs (from 17 to 27 cases, a 58.8% increase). Detection rates for ESBL producers and VRE also improved significantly (by 20%). Pre-enrichment is therefore a valuable addition to routine MDR bacteria screening protocols, which is particularly useful when high accuracy is needed for prevention and infection control as well as for outbreak management.

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来源期刊

European Journal of Clinical Microbiology & Infectious Diseases 医学-传染病学

CiteScore

10.40

自引率

2.20%

发文量

138

审稿时长

1 months

期刊介绍： EJCMID is an interdisciplinary journal devoted to the publication of communications on infectious diseases of bacterial, viral and parasitic origin.