紫杉醇与长春花碱、紫杉醇对PC-3型前列腺癌细胞的细胞毒性比较

IF 3.4 4区 医学 Q2 ONCOLOGY
Ana I García-Pérez, Virginia Rubio, Angel Herráez, Lilian Puebla, José C Diez
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引用次数: 0

摘要

背景/目的:转移性前列腺癌是治疗耐药的人类肿瘤之一。PC-3是一种不表达雄激素受体的常用实验细胞系。我们比较了艾斯维素与长春花碱和紫杉醇对前列腺肿瘤PC-3细胞的细胞毒性。方法:细胞分别用esculetin(100或250 μM)、vinblastine (50 μM)或紫杉醇(100或200 μM)处理19 ~ 72 h,评估细胞代谢活力、膜完整性、DNA断裂和细胞周期分析。用膜联蛋白和碘化丙啶检测细胞凋亡。结果:Esculetin降低PC-3细胞代谢活性呈时间和浓度依赖性。长春花碱和紫杉醇处理细胞的代谢活性不表现出时间依赖性。用250µM长春花素处理48或72 h的细胞,其凋亡水平与用50µM长春花素或用200µM紫杉醇处理19 h的细胞凋亡水平相似。在培养19 h后,长春花素和紫杉醇使细胞周期阻滞在G2/M期。相反,紫杉醇对细胞周期没有显著影响。结论:esculetin对PC-3前列腺细胞的作用可能存在差异。这可能与抵抗性前列腺癌的新疗法有关。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Cytotoxicity of Esculetin Compared with Vinblastine and Paclitaxel in PC-3 Prostate Cancer Cells.

Background/objectives: Metastatic prostate cancer is among the therapy-resistant human neoplasms. PC-3 is a commonly used experimental cell line that does not express androgen receptors. We compared the cytotoxicity of esculetin with that of vinblastine and paclitaxel on prostatic tumour PC-3 cells.

Methods: Cells were treated with either esculetin (100 or 250 μM), vinblastine (50 μM) or paclitaxel (100 or 200 μM) for 19 to 72 h. Cells were assessed for metabolic viability, membrane integrity, DNA fragmentation and cell cycle analysis. Apoptosis was checked with annexin and propidium iodide.

Results: Esculetin decreased the metabolic activity of PC-3 cells in a time- and concentration-dependent way. The metabolic activity of vinblastine- and paclitaxel-treated cells did not show time-dependence. Cells treated with 250 µM esculetin for 48 or 72 h showed apoptosis levels similar to those produced by 50 µM vinblastine at these incubation times or by 200 µM paclitaxel at 19 h. Vinblastine and paclitaxel produced cell cycle arrest in the G2/M phase after incubation for 19 h. In contrast, esculetin did not significantly affect the cell cycle.

Conclusions: A differential action of esculetin on PC-3 prostate cells may be inferred. This may be relevant for novel therapies against resistant prostate cancer.

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来源期刊
Current oncology
Current oncology ONCOLOGY-
CiteScore
3.30
自引率
7.70%
发文量
664
审稿时长
1 months
期刊介绍: Current Oncology is a peer-reviewed, Canadian-based and internationally respected journal. Current Oncology represents a multidisciplinary medium encompassing health care workers in the field of cancer therapy in Canada to report upon and to review progress in the management of this disease. We encourage submissions from all fields of cancer medicine, including radiation oncology, surgical oncology, medical oncology, pediatric oncology, pathology, and cancer rehabilitation and survivorship. Articles published in the journal typically contain information that is relevant directly to clinical oncology practice, and have clear potential for application to the current or future practice of cancer medicine.
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