屠宰场肉芽肿诊断牛结核的qPCR方法比较。

IF 1.9 4区 生物学 Q3 MICROBIOLOGY
Juliana Fernandes de Paula Castro, Gisele Oliveira de Souza, Sueli Akemi Taniwaki, Marcos Bryan Heinemann, José Soares Ferreira Neto
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引用次数: 0

摘要

为了改进对屠宰场处置的牛尸体病变的牛结核病(bTB)的直接诊断——这是在监测系统内识别受感染猪场的主要方法——根据金标准(牛分枝杆菌的培养和鉴定)对两种qPCR测定方法进行了评估。马托格罗索州和圣卡塔琳娜州的检查机构共收集了167份病变样本。样品均质化并使用培养和针对IS1081序列(结核分枝杆菌复合体)和RD4区域(牛分枝杆菌特异性)的qPCR进行分析。两种qPCR检测均表现出可接受的敏感性和特异性,证实了这些靶点的诊断价值。IS1081 qPCR的敏感性为0.80 [95% CI: 0.69-0.89],特异性为0.79 [95% CI: 0.70-0.87]。RD4 qPCR的敏感性为0.74 [95% CI: 0.61-0.84],特异性为0.84 [95% CI: 0.76-0.91]。两种qPCR检测结果的一致性很高(K = 0.89 [95% CI: 0.82-0.96])。当使用培养和IS1081 qPCR的平行结果作为金标准时,RD4检测的灵敏度为0.74 [95% CI: 0.64-0.83],特异性为1.00 [95% CI: 0.96-1.00]。总之,两种分析方法产生的结果具有可比性。由于其对牛分枝杆菌的特异性,RD4 qPCR有望在bTB监测中取代经典细菌学和传统PCR,具有操作优势。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Comparison of qPCR methods for the diagnosis of bovine tuberculosis from granulomas collected at slaughterhouses.

To improve the direct diagnosis of bovine tuberculosis (bTB) in lesions from carcasses condemned at slaughterhouses - the primary method for identifying infected farms within the Surveillance System - two qPCR assays were evaluated against the gold standard: culture and identification of Mycobacterium bovis. A total of 167 lesion samples were collected by inspection services in Mato Grosso and Santa Catarina. Samples were homogenized and analyzed using culture and qPCR targeting the IS1081 sequence (for the M. tuberculosis complex) and the RD4 region (specific to M. bovis). Both qPCR assays demonstrated acceptable sensitivity and specificity, confirming the diagnostic value of these targets. The IS1081 qPCR showed a sensitivity of 0.80 [95% CI: 0.69-0.89] and specificity of 0.79 [95% CI: 0.70-0.87]. The RD4 qPCR yielded a sensitivity of 0.74 [95% CI: 0.61-0.84] and specificity of 0.84 [95% CI: 0.76-0.91]. Agreement between the two qPCR assays was high (K = 0.89 [95% CI: 0.82-0.96]). When using the parallel results of culture and IS1081 qPCR as gold standard, the RD4 assay achieved a sensitivity of 0.74 [95% CI: 0.64-0.83] and specificity of 1.00 [95% CI: 0.96-1.00]. In conclusion, both assays produced comparable results. The RD4 qPCR, due to its specificity for M. bovis, shows promise as a replacement for classical bacteriology and conventional PCR in bTB surveillance, offering operational advantages.

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来源期刊
Brazilian Journal of Microbiology
Brazilian Journal of Microbiology 生物-微生物学
CiteScore
4.10
自引率
4.50%
发文量
216
审稿时长
1.0 months
期刊介绍: The Brazilian Journal of Microbiology is an international peer reviewed journal that covers a wide-range of research on fundamental and applied aspects of microbiology. The journal considers for publication original research articles, short communications, reviews, and letters to the editor, that may be submitted to the following sections: Biotechnology and Industrial Microbiology, Food Microbiology, Bacterial and Fungal Pathogenesis, Clinical Microbiology, Environmental Microbiology, Veterinary Microbiology, Fungal and Bacterial Physiology, Bacterial, Fungal and Virus Molecular Biology, Education in Microbiology. For more details on each section, please check out the instructions for authors. The journal is the official publication of the Brazilian Society of Microbiology and currently publishes 4 issues per year.
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