食用燕窝对小鼠创面愈合的形态学和组织病理学研究。

IF 2.2 4区 生物学 Q3 CELL BIOLOGY
Min Li, Bo Liu, Man Yuan, Xiaoli Dai, Shuang Qiu, Xiangkui Zhang, Xiaoyang Zheng, Kai Chen, Chonggang Duan, Daizhou Zhang, Dongliang Wang, Xiangjing Meng
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引用次数: 0

摘要

食用燕窝(EBN)具有多种药理作用,如提高免疫力、抗氧化、促进大脑发育、预防神经、退行性疾病、美白保湿皮肤等,但其在伤口愈合中的作用尚不清楚。本研究采用小鼠背部全层切除创面建立创面模型。小鼠分别用阳性药物EBN和0.9% NaCl治疗14 d。在损伤后第3、7、14天采集小鼠创伤性皮肤和肠道内容物。采用ELISA、western blot和定量PCR检测炎症因子、蛋白和mRNA的表达。对小鼠损伤皮肤进行组织病理学分析。采用生化实验对羟脯氨酸含量进行测定。采用气相色谱仪检测小鼠肠道内容物。结果表明,EBN能缩小创面面积,降低炎症因子的表达,上调胶原蛋白和α-SMA的表达,改善受损皮肤结构,增加scfa含量,促进创面快速恢复。目前的研究结果可能表明,EBN可能作为一种潜在的有效物质用于伤口愈合治疗,无论是口服还是外用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Morphological and histopathological study of edible bird’s nest on wound healing in mice

Edible bird’s nest (EBN) has a variety of pharmacological effects, such as improving immunity, antioxidant, promoting brain development, nerve prevention, degenerative diseases, and whitening and moisturizing skin, but its role in wound healing is still unknown. In this study, full-thickness excisional wounds were made on the back of mice to establish the wound model. The mice were treated with EBN, a positive drug, and 0.9% NaCl for 14 days. At days 3, 7, and 14 post-injury, the traumatic skin and intestinal contents of mice were collected. ELISA, western blot and quantitative PCR assay were used to evaluate the expression of inflammatory factor, protein, and mRNA. The damaged skin of the mice was analyzed by histopathology. Biochemical experiment was used to evaluate the content of hydroxyproline. Gas chromatograph was used to detect mouse intestinal contents. Results showed that EBN can narrow the wound area, reduce the expression of inflammatory factors, upregulate the expression of collagen and α-SMA, improve the damaged skin structure, increase the content of SCFAs and promote wound recovery rapidly. Current findings may suggest that EBN might serve as a potential effective substance for wound healing therapy either orally or externally.

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来源期刊
Journal of Molecular Histology
Journal of Molecular Histology 生物-细胞生物学
CiteScore
5.90
自引率
0.00%
发文量
68
审稿时长
1 months
期刊介绍: The Journal of Molecular Histology publishes results of original research on the localization and expression of molecules in animal cells, tissues and organs. Coverage includes studies describing novel cellular or ultrastructural distributions of molecules which provide insight into biochemical or physiological function, development, histologic structure and disease processes. Major research themes of particular interest include: - Cell-Cell and Cell-Matrix Interactions; - Connective Tissues; - Development and Disease; - Neuroscience. Please note that the Journal of Molecular Histology does not consider manuscripts dealing with the application of immunological or other probes on non-standard laboratory animal models unless the results are clearly of significant and general biological importance. The Journal of Molecular Histology publishes full-length original research papers, review articles, short communications and letters to the editors. All manuscripts are typically reviewed by two independent referees. The Journal of Molecular Histology is a continuation of The Histochemical Journal.
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