{"title":"碱处理后谷物淀粉淀粉粒相关蛋白的鉴定与表征","authors":"Zekun Xu, , , Zhiqian Wu, , , Xinyu Zhang, , , Mengting Ma, , , Zhongquan Sui*, , and , Harold Corke, ","doi":"10.1021/acs.biomac.5c01158","DOIUrl":null,"url":null,"abstract":"<p >Starch granule-associated proteins (SGAPs) of four cereal starches subjected to different alkali treatments were investigated. Starch synthesis-related proteins were identified as the major SGAPs in normal and waxy maize starches. In rice starch, seed storage proteins were the majority of SGAPs. A concentric ring pattern of the SGAPs distribution was found in normal maize starch and normal rice starch, related to the granule-bound starch synthases. SGAPs of waxy maize starch mainly occur in starch channels, identified as starch synthases and starch branching enzymes. Alkali treatment decreased SGAPs by facilitating protein solubility and degrading the protein structure. Hydrothermal treatment promoted the effect of the alkali. After alkali treatment, the proportion of starch synthesis-related proteins in total SGAPs significantly increased, indicating that these were more resistant to alkali, as they were tightly bound to starch granules. This study revealed the heterogeneity of SGAPs in starch granules and their response to the alkaline treatment.</p>","PeriodicalId":30,"journal":{"name":"Biomacromolecules","volume":"26 10","pages":"6843–6853"},"PeriodicalIF":5.4000,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Identification and Characterization of the Starch Granule-Associated Proteins of Cereal Starches after Alkali Treatment\",\"authors\":\"Zekun Xu, , , Zhiqian Wu, , , Xinyu Zhang, , , Mengting Ma, , , Zhongquan Sui*, , and , Harold Corke, \",\"doi\":\"10.1021/acs.biomac.5c01158\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p >Starch granule-associated proteins (SGAPs) of four cereal starches subjected to different alkali treatments were investigated. Starch synthesis-related proteins were identified as the major SGAPs in normal and waxy maize starches. In rice starch, seed storage proteins were the majority of SGAPs. A concentric ring pattern of the SGAPs distribution was found in normal maize starch and normal rice starch, related to the granule-bound starch synthases. SGAPs of waxy maize starch mainly occur in starch channels, identified as starch synthases and starch branching enzymes. Alkali treatment decreased SGAPs by facilitating protein solubility and degrading the protein structure. Hydrothermal treatment promoted the effect of the alkali. After alkali treatment, the proportion of starch synthesis-related proteins in total SGAPs significantly increased, indicating that these were more resistant to alkali, as they were tightly bound to starch granules. This study revealed the heterogeneity of SGAPs in starch granules and their response to the alkaline treatment.</p>\",\"PeriodicalId\":30,\"journal\":{\"name\":\"Biomacromolecules\",\"volume\":\"26 10\",\"pages\":\"6843–6853\"},\"PeriodicalIF\":5.4000,\"publicationDate\":\"2025-09-26\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biomacromolecules\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://pubs.acs.org/doi/10.1021/acs.biomac.5c01158\",\"RegionNum\":2,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biomacromolecules","FirstCategoryId":"92","ListUrlMain":"https://pubs.acs.org/doi/10.1021/acs.biomac.5c01158","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
Identification and Characterization of the Starch Granule-Associated Proteins of Cereal Starches after Alkali Treatment
Starch granule-associated proteins (SGAPs) of four cereal starches subjected to different alkali treatments were investigated. Starch synthesis-related proteins were identified as the major SGAPs in normal and waxy maize starches. In rice starch, seed storage proteins were the majority of SGAPs. A concentric ring pattern of the SGAPs distribution was found in normal maize starch and normal rice starch, related to the granule-bound starch synthases. SGAPs of waxy maize starch mainly occur in starch channels, identified as starch synthases and starch branching enzymes. Alkali treatment decreased SGAPs by facilitating protein solubility and degrading the protein structure. Hydrothermal treatment promoted the effect of the alkali. After alkali treatment, the proportion of starch synthesis-related proteins in total SGAPs significantly increased, indicating that these were more resistant to alkali, as they were tightly bound to starch granules. This study revealed the heterogeneity of SGAPs in starch granules and their response to the alkaline treatment.
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Biomacromolecules is a leading forum for the dissemination of cutting-edge research at the interface of polymer science and biology. Submissions to Biomacromolecules should contain strong elements of innovation in terms of macromolecular design, synthesis and characterization, or in the application of polymer materials to biology and medicine.
Topics covered by Biomacromolecules include, but are not exclusively limited to: sustainable polymers, polymers based on natural and renewable resources, degradable polymers, polymer conjugates, polymeric drugs, polymers in biocatalysis, biomacromolecular assembly, biomimetic polymers, polymer-biomineral hybrids, biomimetic-polymer processing, polymer recycling, bioactive polymer surfaces, original polymer design for biomedical applications such as immunotherapy, drug delivery, gene delivery, antimicrobial applications, diagnostic imaging and biosensing, polymers in tissue engineering and regenerative medicine, polymeric scaffolds and hydrogels for cell culture and delivery.
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