{"title":"无源孔板嗅探仪在鉴别屎壳虫粪便中药物存在的适用性","authors":"Agustina Escudero, Gonzalo Suárez Veirano, Patricia González-Vainer","doi":"10.1007/s00049-025-00418-9","DOIUrl":null,"url":null,"abstract":"<div><p>Dung beetles are of great importance in livestock ecosystems. The utilization of macrocyclic lactones to control cattle parasites has a detrimental effect on the community of dung invertebrates. The objective of this study was to evaluate the attraction of <i>Onthophagus hircus</i> to ivermectin-fortified fecal matter (IFM) under laboratory conditions. Dung beetles primarily detect food through olfaction. To assess odor discrimination behavior, a passive hole-board olfactometer (HBO) was designed. The efficacy of the design was tested through the execution of two control experiments, which measured the attraction (both group and individual) of <i>O. hircus</i> to control fecal matter (CFM) or soil (S). The third experiment was conducted to evaluate individual attraction to IFM. The data were analyzed using descriptive statistics and comparative analyses, and the selection rate was estimated. In both the individual and group control experiences, active individuals were significantly attracted to CFM (46% and 74%, respectively) (p < 0.05 in both cases). Attraction percentages in experiment 3 were: 44% for IFM, 38% for CFM, and 19% for S (p < 0.05). Male and female beetles showed a slight tendency to be attracted to IFM but did not display a significant preference between IFM and CFM (p > 0.05). This study confirmed that an HBO design is a valid approach for testing odor discrimination and assessing the attraction of <i>O. hircus</i> to bovine feces. However, this species demonstrated an inability to differentiate between ivermectin-contaminated and untreated faeces.</p></div>","PeriodicalId":515,"journal":{"name":"Chemoecology","volume":"35 2","pages":"89 - 96"},"PeriodicalIF":1.3000,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"The applicability of a passive hole-board olfactometer in the discrimination of the presence of drugs in fecal matter for dung beetles\",\"authors\":\"Agustina Escudero, Gonzalo Suárez Veirano, Patricia González-Vainer\",\"doi\":\"10.1007/s00049-025-00418-9\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Dung beetles are of great importance in livestock ecosystems. The utilization of macrocyclic lactones to control cattle parasites has a detrimental effect on the community of dung invertebrates. The objective of this study was to evaluate the attraction of <i>Onthophagus hircus</i> to ivermectin-fortified fecal matter (IFM) under laboratory conditions. Dung beetles primarily detect food through olfaction. To assess odor discrimination behavior, a passive hole-board olfactometer (HBO) was designed. The efficacy of the design was tested through the execution of two control experiments, which measured the attraction (both group and individual) of <i>O. hircus</i> to control fecal matter (CFM) or soil (S). The third experiment was conducted to evaluate individual attraction to IFM. The data were analyzed using descriptive statistics and comparative analyses, and the selection rate was estimated. In both the individual and group control experiences, active individuals were significantly attracted to CFM (46% and 74%, respectively) (p < 0.05 in both cases). Attraction percentages in experiment 3 were: 44% for IFM, 38% for CFM, and 19% for S (p < 0.05). Male and female beetles showed a slight tendency to be attracted to IFM but did not display a significant preference between IFM and CFM (p > 0.05). This study confirmed that an HBO design is a valid approach for testing odor discrimination and assessing the attraction of <i>O. hircus</i> to bovine feces. However, this species demonstrated an inability to differentiate between ivermectin-contaminated and untreated faeces.</p></div>\",\"PeriodicalId\":515,\"journal\":{\"name\":\"Chemoecology\",\"volume\":\"35 2\",\"pages\":\"89 - 96\"},\"PeriodicalIF\":1.3000,\"publicationDate\":\"2025-02-20\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Chemoecology\",\"FirstCategoryId\":\"93\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s00049-025-00418-9\",\"RegionNum\":3,\"RegionCategory\":\"环境科学与生态学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Chemoecology","FirstCategoryId":"93","ListUrlMain":"https://link.springer.com/article/10.1007/s00049-025-00418-9","RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
The applicability of a passive hole-board olfactometer in the discrimination of the presence of drugs in fecal matter for dung beetles
Dung beetles are of great importance in livestock ecosystems. The utilization of macrocyclic lactones to control cattle parasites has a detrimental effect on the community of dung invertebrates. The objective of this study was to evaluate the attraction of Onthophagus hircus to ivermectin-fortified fecal matter (IFM) under laboratory conditions. Dung beetles primarily detect food through olfaction. To assess odor discrimination behavior, a passive hole-board olfactometer (HBO) was designed. The efficacy of the design was tested through the execution of two control experiments, which measured the attraction (both group and individual) of O. hircus to control fecal matter (CFM) or soil (S). The third experiment was conducted to evaluate individual attraction to IFM. The data were analyzed using descriptive statistics and comparative analyses, and the selection rate was estimated. In both the individual and group control experiences, active individuals were significantly attracted to CFM (46% and 74%, respectively) (p < 0.05 in both cases). Attraction percentages in experiment 3 were: 44% for IFM, 38% for CFM, and 19% for S (p < 0.05). Male and female beetles showed a slight tendency to be attracted to IFM but did not display a significant preference between IFM and CFM (p > 0.05). This study confirmed that an HBO design is a valid approach for testing odor discrimination and assessing the attraction of O. hircus to bovine feces. However, this species demonstrated an inability to differentiate between ivermectin-contaminated and untreated faeces.
期刊介绍：
It is the aim of Chemoecology to promote and stimulate basic science in the field of chemical ecology by publishing research papers that integrate evolution and/or ecology and chemistry in an attempt to increase our understanding of the biological significance of natural products. Its scopes cover the evolutionary biology, mechanisms and chemistry of biotic interactions and the evolution and synthesis of the underlying natural products. Manuscripts on the evolution and ecology of trophic relationships, intra- and interspecific communication, competition, and other kinds of chemical communication in all types of organismic interactions will be considered suitable for publication. Ecological studies of trophic interactions will be considered also if they are based on the information of the transmission of natural products (e.g. fatty acids) through the food-chain. Chemoecology further publishes papers that relate to the evolution and ecology of interactions mediated by non-volatile compounds (e.g. adhesive secretions). Mechanistic approaches may include the identification, biosynthesis and metabolism of substances that carry information and the elucidation of receptor- and transduction systems using physiological, biochemical and molecular techniques. Papers describing the structure and functional morphology of organs involved in chemical communication will also be considered.
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