{"title":"绿色合成的海藻酸盐包覆银纳米粒子与多西紫杉醇药物结合：x射线照射下LNCaP前列腺癌细胞的联合治疗","authors":"Benyamin Keshavarz, Mahmoud Gharbavi, Ghasem Bagherpour, Hamed Rezaeejam, Behrooz Johari","doi":"10.1007/s10924-025-03591-8","DOIUrl":null,"url":null,"abstract":"<div><p>This investigation examines an innovative combinatorial therapeutic strategy for prostate carcinoma utilizing silver nanoparticles (AgNPs) that are enveloped with alginate macromolecules, conjugated with docetaxel (DTX) drug, and administered in conjunction with X-irradiation. The resultant AgNPs@Alg-DTX nanocomposites, wherein alginate functions as a polymeric coating, underwent comprehensive characterization <i>via</i> a variety of methodologies, encompassing FT-IR and UV-vis spectroscopy, DLS, FE-SEM, EDS, TEM, hemolysis, and release tests. The cellular uptake and anti-tumor efficacy were appraised through flow cytometry, MTT, apoptosis, and scratch assays. The physicochemical characterizations substantiated the successful synthesis and suitable dimensions of the nanocomposites, with alginate macromolecules proficiently coating the AgNPs. Kinetics of drug release exhibited a time-dependent profile, culminating at the 24-hour mark. Findings illustrated that AgNPs@Alg-DTX, characterized by the alginate macromolecular coating, were effectively internalized by LNCaP cells. The radiosensitizing capabilities of the alginate-coated AgNPs significantly amplified the efficacy of X-irradiation. This innovative methodology, which integrates alginate-coated AgNPs conjugated with DTX and radiotherapy, presents considerable promise for prostate cancer therapy by apoptosis induction and cell growth inhibition. The study advocates for further investigation of this therapeutic paradigm, capitalizing on the advantages of alginate macromolecules as a nanoparticle coating, for prospective clinical applications in the treatment of prostate cancer.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":659,"journal":{"name":"Journal of Polymers and the Environment","volume":"33 7","pages":"3029 - 3049"},"PeriodicalIF":5.0000,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Green-Synthesized Silver Nanoparticles Coated with Alginate and Conjugated to Docetaxel Drug: Combination Therapy Under X-Irradiation on LNCaP Prostate Cancer Cells\",\"authors\":\"Benyamin Keshavarz, Mahmoud Gharbavi, Ghasem Bagherpour, Hamed Rezaeejam, Behrooz Johari\",\"doi\":\"10.1007/s10924-025-03591-8\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>This investigation examines an innovative combinatorial therapeutic strategy for prostate carcinoma utilizing silver nanoparticles (AgNPs) that are enveloped with alginate macromolecules, conjugated with docetaxel (DTX) drug, and administered in conjunction with X-irradiation. The resultant AgNPs@Alg-DTX nanocomposites, wherein alginate functions as a polymeric coating, underwent comprehensive characterization <i>via</i> a variety of methodologies, encompassing FT-IR and UV-vis spectroscopy, DLS, FE-SEM, EDS, TEM, hemolysis, and release tests. The cellular uptake and anti-tumor efficacy were appraised through flow cytometry, MTT, apoptosis, and scratch assays. The physicochemical characterizations substantiated the successful synthesis and suitable dimensions of the nanocomposites, with alginate macromolecules proficiently coating the AgNPs. Kinetics of drug release exhibited a time-dependent profile, culminating at the 24-hour mark. Findings illustrated that AgNPs@Alg-DTX, characterized by the alginate macromolecular coating, were effectively internalized by LNCaP cells. The radiosensitizing capabilities of the alginate-coated AgNPs significantly amplified the efficacy of X-irradiation. This innovative methodology, which integrates alginate-coated AgNPs conjugated with DTX and radiotherapy, presents considerable promise for prostate cancer therapy by apoptosis induction and cell growth inhibition. The study advocates for further investigation of this therapeutic paradigm, capitalizing on the advantages of alginate macromolecules as a nanoparticle coating, for prospective clinical applications in the treatment of prostate cancer.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>\",\"PeriodicalId\":659,\"journal\":{\"name\":\"Journal of Polymers and the Environment\",\"volume\":\"33 7\",\"pages\":\"3029 - 3049\"},\"PeriodicalIF\":5.0000,\"publicationDate\":\"2025-05-15\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Polymers and the Environment\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s10924-025-03591-8\",\"RegionNum\":3,\"RegionCategory\":\"工程技术\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"ENGINEERING, ENVIRONMENTAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Polymers and the Environment","FirstCategoryId":"5","ListUrlMain":"https://link.springer.com/article/10.1007/s10924-025-03591-8","RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ENGINEERING, ENVIRONMENTAL","Score":null,"Total":0}
Green-Synthesized Silver Nanoparticles Coated with Alginate and Conjugated to Docetaxel Drug: Combination Therapy Under X-Irradiation on LNCaP Prostate Cancer Cells
This investigation examines an innovative combinatorial therapeutic strategy for prostate carcinoma utilizing silver nanoparticles (AgNPs) that are enveloped with alginate macromolecules, conjugated with docetaxel (DTX) drug, and administered in conjunction with X-irradiation. The resultant AgNPs@Alg-DTX nanocomposites, wherein alginate functions as a polymeric coating, underwent comprehensive characterization via a variety of methodologies, encompassing FT-IR and UV-vis spectroscopy, DLS, FE-SEM, EDS, TEM, hemolysis, and release tests. The cellular uptake and anti-tumor efficacy were appraised through flow cytometry, MTT, apoptosis, and scratch assays. The physicochemical characterizations substantiated the successful synthesis and suitable dimensions of the nanocomposites, with alginate macromolecules proficiently coating the AgNPs. Kinetics of drug release exhibited a time-dependent profile, culminating at the 24-hour mark. Findings illustrated that AgNPs@Alg-DTX, characterized by the alginate macromolecular coating, were effectively internalized by LNCaP cells. The radiosensitizing capabilities of the alginate-coated AgNPs significantly amplified the efficacy of X-irradiation. This innovative methodology, which integrates alginate-coated AgNPs conjugated with DTX and radiotherapy, presents considerable promise for prostate cancer therapy by apoptosis induction and cell growth inhibition. The study advocates for further investigation of this therapeutic paradigm, capitalizing on the advantages of alginate macromolecules as a nanoparticle coating, for prospective clinical applications in the treatment of prostate cancer.
期刊介绍：
The Journal of Polymers and the Environment fills the need for an international forum in this diverse and rapidly expanding field. The journal serves a crucial role for the publication of information from a wide range of disciplines and is a central outlet for the publication of high-quality peer-reviewed original papers, review articles and short communications. The journal is intentionally interdisciplinary in regard to contributions and covers the following subjects - polymers, environmentally degradable polymers, and degradation pathways: biological, photochemical, oxidative and hydrolytic; new environmental materials: derived by chemical and biosynthetic routes; environmental blends and composites; developments in processing and reactive processing of environmental polymers; characterization of environmental materials: mechanical, physical, thermal, rheological, morphological, and others; recyclable polymers and plastics recycling environmental testing: in-laboratory simulations, outdoor exposures, and standardization of methodologies; environmental fate: end products and intermediates of biodegradation; microbiology and enzymology of polymer biodegradation; solid-waste management and public legislation specific to environmental polymers; and other related topics.
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