Sung-Wook Nam, Hana Lee, Dong-Gyu Jeon, Mi-Young Son
{"title":"三维类器官培养装置诱导体外人体肠道炎症模型","authors":"Sung-Wook Nam, Hana Lee, Dong-Gyu Jeon, Mi-Young Son","doi":"10.1007/s13391-025-00561-z","DOIUrl":null,"url":null,"abstract":"<div><p>Organoid culturing in microfluidic devices becomes an important technology for high throughput screening (HTS) of drugs, nutrients, and microbiome metabolites. Here, we present 3D organoid culturing devices to induce inflammation in human pluripotent stem cell (hPSC)-derived intestinal organoids (hIOs). Using high resolution 3D printing process, we established a method to fabricate microfluidic devices which have microchannels and 3D dome. The 3D dome geometry is suitable for the formation of Matrigel which serves as a cellular matrix. To validate the efficacy of the culturing devices, we induced inflammation in the hPSC-derived hIOs by the treatment of the combined proinflammatory cytokines such as interferon γ (IFNγ) and tumor necrosis factor α (TNFα). The inflamed hIOs have thinner epithelium with the upregulation of inflammatory cytokines. The induction of inflammatory models inside the 3D devices paves a way towards a microfluidic platform to investigate HTS.</p><h3>Graphic Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":536,"journal":{"name":"Electronic Materials Letters","volume":"21 4","pages":"504 - 512"},"PeriodicalIF":2.6000,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"3D Organoid Culturing Devices to Induce in Vitro Models of Human Intestinal Inflammation\",\"authors\":\"Sung-Wook Nam, Hana Lee, Dong-Gyu Jeon, Mi-Young Son\",\"doi\":\"10.1007/s13391-025-00561-z\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Organoid culturing in microfluidic devices becomes an important technology for high throughput screening (HTS) of drugs, nutrients, and microbiome metabolites. Here, we present 3D organoid culturing devices to induce inflammation in human pluripotent stem cell (hPSC)-derived intestinal organoids (hIOs). Using high resolution 3D printing process, we established a method to fabricate microfluidic devices which have microchannels and 3D dome. The 3D dome geometry is suitable for the formation of Matrigel which serves as a cellular matrix. To validate the efficacy of the culturing devices, we induced inflammation in the hPSC-derived hIOs by the treatment of the combined proinflammatory cytokines such as interferon γ (IFNγ) and tumor necrosis factor α (TNFα). The inflamed hIOs have thinner epithelium with the upregulation of inflammatory cytokines. The induction of inflammatory models inside the 3D devices paves a way towards a microfluidic platform to investigate HTS.</p><h3>Graphic Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>\",\"PeriodicalId\":536,\"journal\":{\"name\":\"Electronic Materials Letters\",\"volume\":\"21 4\",\"pages\":\"504 - 512\"},\"PeriodicalIF\":2.6000,\"publicationDate\":\"2025-03-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Electronic Materials Letters\",\"FirstCategoryId\":\"88\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s13391-025-00561-z\",\"RegionNum\":4,\"RegionCategory\":\"材料科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"MATERIALS SCIENCE, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Electronic Materials Letters","FirstCategoryId":"88","ListUrlMain":"https://link.springer.com/article/10.1007/s13391-025-00561-z","RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"MATERIALS SCIENCE, MULTIDISCIPLINARY","Score":null,"Total":0}
3D Organoid Culturing Devices to Induce in Vitro Models of Human Intestinal Inflammation
Organoid culturing in microfluidic devices becomes an important technology for high throughput screening (HTS) of drugs, nutrients, and microbiome metabolites. Here, we present 3D organoid culturing devices to induce inflammation in human pluripotent stem cell (hPSC)-derived intestinal organoids (hIOs). Using high resolution 3D printing process, we established a method to fabricate microfluidic devices which have microchannels and 3D dome. The 3D dome geometry is suitable for the formation of Matrigel which serves as a cellular matrix. To validate the efficacy of the culturing devices, we induced inflammation in the hPSC-derived hIOs by the treatment of the combined proinflammatory cytokines such as interferon γ (IFNγ) and tumor necrosis factor α (TNFα). The inflamed hIOs have thinner epithelium with the upregulation of inflammatory cytokines. The induction of inflammatory models inside the 3D devices paves a way towards a microfluidic platform to investigate HTS.
期刊介绍：
Electronic Materials Letters is an official journal of the Korean Institute of Metals and Materials. It is a peer-reviewed international journal publishing print and online version. It covers all disciplines of research and technology in electronic materials. Emphasis is placed on science, engineering and applications of advanced materials, including electronic, magnetic, optical, organic, electrochemical, mechanical, and nanoscale materials. The aspects of synthesis and processing include thin films, nanostructures, self assembly, and bulk, all related to thermodynamics, kinetics and/or modeling.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
