利用混合多路荧光和磁共振成像(HyFMRI)对并发神经元、星形细胞和血流动力学活动进行无创大规模成像。

IF 23.4 Q1 OPTICS
Zhenyue Chen,Yi Chen,Irmak Gezginer,Qingxiang Ding,Hikari A I Yoshihara,Xosé Luís Deán-Ben,Ruiqing Ni,Daniel Razansky
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引用次数: 0

摘要

目前,对于星形胶质细胞在神经血管耦合及其与其他脑细胞的功能联系中的作用的系统理解和实验验证存在一个关键的空白。尽管对于多尺度脑问题的功能性神经成像工具有广泛的选择,但目前还没有一种方法可以在大范围内识别神经和胶质细胞的反应,同时绘制相关的血流动力学活动。我们提出了一种混合多路荧光和磁共振成像(HyFMRI)平台,用于测量神经元和星形胶质细胞活动,同时记录全脑血流动力学反应。它的特点是基于纤维镜的成像系统,用于多通道荧光和光学固有信号记录,以及一个定制的表面射频线圈,该线圈被整合到临床前磁共振成像(MRI)扫描仪的孔中。我们使用HyFMRI来研究外周刺激引起的大脑反应,这些反应是由RCaMP和GCaMP基因编码的钙指标不同标记的。刺激引起的神经元反应表现出最快的动力学和最高的激活幅度,其次是星形细胞信号和血流动力学反应。此外,神经元和星形胶质细胞的激活痕迹表现出高度的线性相关性,从而为星形胶质细胞介导神经血管耦合提供了直接证据。这种新开发的捕捉细胞类型特异性钙信号和全脑血流动力学的能力,使得在健康和疾病中同时研究神经-胶质-血管相互作用成为可能。因此,HyFMRI扩展了当前的神经成像工具箱,用于突触可塑性、神经回路、脑功能和疾病的广泛研究。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Non-invasive large-scale imaging of concurrent neuronal, astrocytic, and hemodynamic activity with hybrid multiplexed fluorescence and magnetic resonance imaging (HyFMRI).
A critical gap currently exists in systematic understanding and experimental validation of the role of astrocytes in neurovascular coupling and their functional links with other brain cells. Despite a broad selection of functional neuroimaging tools for multi-scale brain interrogations, no methodology currently exists that can discern responses from neural and glial cells while simultaneously mapping the associated hemodynamic activity on a large scale. We present a hybrid multiplexed fluorescence and magnetic resonance imaging (HyFMRI) platform for measuring neuronal and astrocytic activity registered to concurrently recorded brain-wide hemodynamic responses. It features a fiberscope-based imaging system for multichannel fluorescence and optical intrinsic signal recordings and a custom surface radiofrequency coil, which are incorporated into the bore of a preclinical magnetic resonance imaging (MRI) scanner. We used HyFMRI to study peripheral-stimulus-evoked brain responses in mice differentially labeled with RCaMP and GCaMP genetically-encoded calcium indicators. Stimulation-evoked neuronal responses displayed the fastest kinetics and highest activation amplitude followed by astrocytic signals and the hemodynamic responses simultaneously recorded with functional MRI. In addition, the activation traces from neurons and astrocytes exhibited high linear correlation, thus providing direct evidence of astrocytic mediation in neurovascular coupling. This newly developed capacity to capture cell-type-specific calcium signaling alongside whole-brain hemodynamics enables the simultaneous investigation of neuro-glial-vascular interactions in health and disease. HyFMRI thus expands the current neuroimaging toolbox for a wide range of studies into synaptic plasticity, neural circuitry, brain function and disorders.
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来源期刊
Light-Science & Applications
Light-Science & Applications 数理科学, 物理学I, 光学, 凝聚态物性 II :电子结构、电学、磁学和光学性质, 无机非金属材料, 无机非金属类光电信息与功能材料, 工程与材料, 信息科学, 光学和光电子学, 光学和光电子材料, 非线性光学与量子光学
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803
审稿时长
2.1 months
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