{"title":"日本野禽耐药大肠杆菌的流行及遗传特征。","authors":"Tetsuo Asai, Michiyo Sugiyama, Tomoya Morimoto, Akiko Sudo, Junji Moribe, Masaru Usui","doi":"10.1292/jvms.25-0336","DOIUrl":null,"url":null,"abstract":"<p><p>Antimicrobial-resistant bacteria in wild animals are a multisectoral concern worldwide. In this study, we examined fecal samples collected from wild birds in two prefectures of Japan between 2021 and 2024 using both deoxycholate-hydrogen sulfide-lactose (DHL) agar media and antimicrobial-containing DHL agar media. Of the 252 fecal samples from 11 bird species, 159 E. coli were isolated from 70 samples (27.8%) of six species, and resistance was found in 4.4% of isolates from DHL media. Using nalidixic acid (NAL)- and cefotaxime (CTX)-containing media, NAL-resistant isolates were isolated from great cormorants and spot-billed ducks, and an extended-spectrum β-lactamase (ESBL)/AmpC β-lactamase producer was isolated from great cormorants. Next-generation sequencing analysis of 19 E. coli isolates exhibiting resistance to NAL and/or CTX indicated diverse genotypes. Genetic analysis indicated that amino acid substitution of quinolone-resistance-determining-regions and qnrS were responsible for NAL resistance, and CTX-Ms (bla<sub>CTX-M-14</sub> in three isolates, and bla<sub>CTX-M-15</sub> and bla<sub>CTX-M-55</sub> in each) and AmpC β-lactamase (bla<sub>MOX-4</sub> and bla<sub>CMY-2</sub> in each) were responsible for CTX resistance. Despite the presence of certain resistant strains, the overall prevalence of antimicrobial-resistant E. coli remains low in wild birds, suggesting limited environmental exposure to antimicrobials and antimicrobial resistant bacteria.</p>","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":" ","pages":""},"PeriodicalIF":1.1000,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Prevalence and genetic characteristics of antimicrobial-resistant Escherichia coli in wild birds in Japan.\",\"authors\":\"Tetsuo Asai, Michiyo Sugiyama, Tomoya Morimoto, Akiko Sudo, Junji Moribe, Masaru Usui\",\"doi\":\"10.1292/jvms.25-0336\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Antimicrobial-resistant bacteria in wild animals are a multisectoral concern worldwide. In this study, we examined fecal samples collected from wild birds in two prefectures of Japan between 2021 and 2024 using both deoxycholate-hydrogen sulfide-lactose (DHL) agar media and antimicrobial-containing DHL agar media. Of the 252 fecal samples from 11 bird species, 159 E. coli were isolated from 70 samples (27.8%) of six species, and resistance was found in 4.4% of isolates from DHL media. Using nalidixic acid (NAL)- and cefotaxime (CTX)-containing media, NAL-resistant isolates were isolated from great cormorants and spot-billed ducks, and an extended-spectrum β-lactamase (ESBL)/AmpC β-lactamase producer was isolated from great cormorants. Next-generation sequencing analysis of 19 E. coli isolates exhibiting resistance to NAL and/or CTX indicated diverse genotypes. Genetic analysis indicated that amino acid substitution of quinolone-resistance-determining-regions and qnrS were responsible for NAL resistance, and CTX-Ms (bla<sub>CTX-M-14</sub> in three isolates, and bla<sub>CTX-M-15</sub> and bla<sub>CTX-M-55</sub> in each) and AmpC β-lactamase (bla<sub>MOX-4</sub> and bla<sub>CMY-2</sub> in each) were responsible for CTX resistance. Despite the presence of certain resistant strains, the overall prevalence of antimicrobial-resistant E. coli remains low in wild birds, suggesting limited environmental exposure to antimicrobials and antimicrobial resistant bacteria.</p>\",\"PeriodicalId\":49959,\"journal\":{\"name\":\"Journal of Veterinary Medical Science\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":1.1000,\"publicationDate\":\"2025-09-23\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Veterinary Medical Science\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1292/jvms.25-0336\",\"RegionNum\":4,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"VETERINARY SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Veterinary Medical Science","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1292/jvms.25-0336","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}
Prevalence and genetic characteristics of antimicrobial-resistant Escherichia coli in wild birds in Japan.
Antimicrobial-resistant bacteria in wild animals are a multisectoral concern worldwide. In this study, we examined fecal samples collected from wild birds in two prefectures of Japan between 2021 and 2024 using both deoxycholate-hydrogen sulfide-lactose (DHL) agar media and antimicrobial-containing DHL agar media. Of the 252 fecal samples from 11 bird species, 159 E. coli were isolated from 70 samples (27.8%) of six species, and resistance was found in 4.4% of isolates from DHL media. Using nalidixic acid (NAL)- and cefotaxime (CTX)-containing media, NAL-resistant isolates were isolated from great cormorants and spot-billed ducks, and an extended-spectrum β-lactamase (ESBL)/AmpC β-lactamase producer was isolated from great cormorants. Next-generation sequencing analysis of 19 E. coli isolates exhibiting resistance to NAL and/or CTX indicated diverse genotypes. Genetic analysis indicated that amino acid substitution of quinolone-resistance-determining-regions and qnrS were responsible for NAL resistance, and CTX-Ms (blaCTX-M-14 in three isolates, and blaCTX-M-15 and blaCTX-M-55 in each) and AmpC β-lactamase (blaMOX-4 and blaCMY-2 in each) were responsible for CTX resistance. Despite the presence of certain resistant strains, the overall prevalence of antimicrobial-resistant E. coli remains low in wild birds, suggesting limited environmental exposure to antimicrobials and antimicrobial resistant bacteria.
期刊介绍：
JVMS is a peer-reviewed journal and publishes a variety of papers on veterinary science from basic research to applied science and clinical research. JVMS is published monthly and consists of twelve issues per year. Papers are from the areas of anatomy, physiology, pharmacology, toxicology, pathology, immunology, microbiology, virology, parasitology, internal medicine, surgery, clinical pathology, theriogenology, avian disease, public health, ethology, and laboratory animal science. Although JVMS has played a role in publishing the scientific achievements of Japanese researchers and clinicians for many years, it now also accepts papers submitted from all over the world.
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