Omima Sayed Mohammed, Abdulmabod Omar, Mohammad AbdElhameed Alwaseef, Hatem Mohamed Newishy, Medhat Ali Salah Abd Elghaffar, Zeinab Adawy, Mahmoud Ahmed Arfa, Tarek Abualkasem Abualwafa Mohammed, Mohamed Abd Elrahman Abd Elkader Elbakery, Usama Abdalla, Hanem Mohamed Badawy, Mohammad Abd Elhalem Abd El Razik, Huda Ali Abdalrahim Alzubir, Fawzia A Sharaf, Bothayna I Saleh, Abdullah Mustafa Ibrahim Gaafar, Bassam Abdellatif Mahmoud Elsayed, Esam M Shawky, Mohamed Mahmoud Abdelfattah Abdelrahman, Mai Mosad Morsy, Mohamed Mounir Ahmed Rizk, Nahla Mohammed Eldeeb, Ahmed Rizk Elkholy, Wageih Salem ElNaghy, Ola A Elshora, Hossam M Farid El-Zamek, Ahmed R I Ali, Mohamed Basiouny Yahia, Fatma Ibrahim Ismail Mayah, Ahmed M Ewis, Eid Mohammed, Ahmed Atef Ganna, Esraa El-Sayed AbdelMaksoud Elmahdy
{"title":"FilmArray肺炎面板加在住院患者院内呼吸道感染管理中的价值：横断面研究。","authors":"Omima Sayed Mohammed, Abdulmabod Omar, Mohammad AbdElhameed Alwaseef, Hatem Mohamed Newishy, Medhat Ali Salah Abd Elghaffar, Zeinab Adawy, Mahmoud Ahmed Arfa, Tarek Abualkasem Abualwafa Mohammed, Mohamed Abd Elrahman Abd Elkader Elbakery, Usama Abdalla, Hanem Mohamed Badawy, Mohammad Abd Elhalem Abd El Razik, Huda Ali Abdalrahim Alzubir, Fawzia A Sharaf, Bothayna I Saleh, Abdullah Mustafa Ibrahim Gaafar, Bassam Abdellatif Mahmoud Elsayed, Esam M Shawky, Mohamed Mahmoud Abdelfattah Abdelrahman, Mai Mosad Morsy, Mohamed Mounir Ahmed Rizk, Nahla Mohammed Eldeeb, Ahmed Rizk Elkholy, Wageih Salem ElNaghy, Ola A Elshora, Hossam M Farid El-Zamek, Ahmed R I Ali, Mohamed Basiouny Yahia, Fatma Ibrahim Ismail Mayah, Ahmed M Ewis, Eid Mohammed, Ahmed Atef Ganna, Esraa El-Sayed AbdelMaksoud Elmahdy","doi":"10.7417/CT.2025.5276","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>The traditional reliance on slow culture-based diagnostics often leads to delays in targeted therapy and the overuse of broad-spectrum antibiotics, aggravating the problem of antimicrobial resistance.</p><p><strong>Aim: </strong>To compare the BioFire, FilmArray Pneumonia Panel plus (FA-PneumobioMérieux) assay with a conventional diagnostic tools, including culture and VITEK 2 (bioMérieux), to identify microorganisms in critically ill patients (severe pulmonary infection) to get the best results within a short period.</p><p><strong>Methods: </strong>An observational, comparative cross-sectional, hospital-based investigation was carried out at the microbiology unit of the clinical pathology department of Alazhar University hospitals. Lower respiratory tract samples were collected from patients with hospital-acquired pneumonia and ICU admissions. Conventional culture was carried out for all specimens. Susceptibility testing was performed on all specimens using the VITEK 2 system (bioMérieux). The BioFire FA-Pneumo assay was conducted according to the manufacturer's instructions.</p><p><strong>Results: </strong>Out of 60 patients with pneumonia 90% of specimens were sputum samples. For all examined pathogens, there was no statistically significant difference between the detection rates of Multiplex PCR and Vitek methods (P-value >0.05). The most prevalent resistant gene detected by Multiplex PCR was gene (blaNDM-1 ), found in 23.3% (n=14) of the samples, followed by OXA-48-like, which was found in 15% (n=9) of the samples. The detection of CTX-, CTX-M, KPC, mecA/C, and MREJ, VIM resistant genes were at lower frequencies.</p><p><strong>Conclusion: </strong>The FilmArray Pneumonia Panel Plus (FA-Pneumo) is a rapid molecular diagnostic tool designed to overcome the limitations of conventional methods by quickly identifying a broad range of pathogens and resistance genes. The capability of FA-Pneumo to rapidly identify both pathogens and key resistance genes provides a significant advantage for clinical decision-making.</p>","PeriodicalId":50686,"journal":{"name":"Clinica Terapeutica","volume":"176 5","pages":"645-650"},"PeriodicalIF":0.0000,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"The Value of FilmArray Pneumonia Panel plus in Managing Nosocomial Respiratory Infections in Hospitalized Patients: Cross-sectional study.\",\"authors\":\"Omima Sayed Mohammed, Abdulmabod Omar, Mohammad AbdElhameed Alwaseef, Hatem Mohamed Newishy, Medhat Ali Salah Abd Elghaffar, Zeinab Adawy, Mahmoud Ahmed Arfa, Tarek Abualkasem Abualwafa Mohammed, Mohamed Abd Elrahman Abd Elkader Elbakery, Usama Abdalla, Hanem Mohamed Badawy, Mohammad Abd Elhalem Abd El Razik, Huda Ali Abdalrahim Alzubir, Fawzia A Sharaf, Bothayna I Saleh, Abdullah Mustafa Ibrahim Gaafar, Bassam Abdellatif Mahmoud Elsayed, Esam M Shawky, Mohamed Mahmoud Abdelfattah Abdelrahman, Mai Mosad Morsy, Mohamed Mounir Ahmed Rizk, Nahla Mohammed Eldeeb, Ahmed Rizk Elkholy, Wageih Salem ElNaghy, Ola A Elshora, Hossam M Farid El-Zamek, Ahmed R I Ali, Mohamed Basiouny Yahia, Fatma Ibrahim Ismail Mayah, Ahmed M Ewis, Eid Mohammed, Ahmed Atef Ganna, Esraa El-Sayed AbdelMaksoud Elmahdy\",\"doi\":\"10.7417/CT.2025.5276\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>The traditional reliance on slow culture-based diagnostics often leads to delays in targeted therapy and the overuse of broad-spectrum antibiotics, aggravating the problem of antimicrobial resistance.</p><p><strong>Aim: </strong>To compare the BioFire, FilmArray Pneumonia Panel plus (FA-PneumobioMérieux) assay with a conventional diagnostic tools, including culture and VITEK 2 (bioMérieux), to identify microorganisms in critically ill patients (severe pulmonary infection) to get the best results within a short period.</p><p><strong>Methods: </strong>An observational, comparative cross-sectional, hospital-based investigation was carried out at the microbiology unit of the clinical pathology department of Alazhar University hospitals. Lower respiratory tract samples were collected from patients with hospital-acquired pneumonia and ICU admissions. Conventional culture was carried out for all specimens. Susceptibility testing was performed on all specimens using the VITEK 2 system (bioMérieux). The BioFire FA-Pneumo assay was conducted according to the manufacturer's instructions.</p><p><strong>Results: </strong>Out of 60 patients with pneumonia 90% of specimens were sputum samples. For all examined pathogens, there was no statistically significant difference between the detection rates of Multiplex PCR and Vitek methods (P-value >0.05). The most prevalent resistant gene detected by Multiplex PCR was gene (blaNDM-1 ), found in 23.3% (n=14) of the samples, followed by OXA-48-like, which was found in 15% (n=9) of the samples. The detection of CTX-, CTX-M, KPC, mecA/C, and MREJ, VIM resistant genes were at lower frequencies.</p><p><strong>Conclusion: </strong>The FilmArray Pneumonia Panel Plus (FA-Pneumo) is a rapid molecular diagnostic tool designed to overcome the limitations of conventional methods by quickly identifying a broad range of pathogens and resistance genes. The capability of FA-Pneumo to rapidly identify both pathogens and key resistance genes provides a significant advantage for clinical decision-making.</p>\",\"PeriodicalId\":50686,\"journal\":{\"name\":\"Clinica Terapeutica\",\"volume\":\"176 5\",\"pages\":\"645-650\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2025-09-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Clinica Terapeutica\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.7417/CT.2025.5276\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"Medicine\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Clinica Terapeutica","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.7417/CT.2025.5276","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"Medicine","Score":null,"Total":0}
The Value of FilmArray Pneumonia Panel plus in Managing Nosocomial Respiratory Infections in Hospitalized Patients: Cross-sectional study.
Background: The traditional reliance on slow culture-based diagnostics often leads to delays in targeted therapy and the overuse of broad-spectrum antibiotics, aggravating the problem of antimicrobial resistance.
Aim: To compare the BioFire, FilmArray Pneumonia Panel plus (FA-PneumobioMérieux) assay with a conventional diagnostic tools, including culture and VITEK 2 (bioMérieux), to identify microorganisms in critically ill patients (severe pulmonary infection) to get the best results within a short period.
Methods: An observational, comparative cross-sectional, hospital-based investigation was carried out at the microbiology unit of the clinical pathology department of Alazhar University hospitals. Lower respiratory tract samples were collected from patients with hospital-acquired pneumonia and ICU admissions. Conventional culture was carried out for all specimens. Susceptibility testing was performed on all specimens using the VITEK 2 system (bioMérieux). The BioFire FA-Pneumo assay was conducted according to the manufacturer's instructions.
Results: Out of 60 patients with pneumonia 90% of specimens were sputum samples. For all examined pathogens, there was no statistically significant difference between the detection rates of Multiplex PCR and Vitek methods (P-value >0.05). The most prevalent resistant gene detected by Multiplex PCR was gene (blaNDM-1 ), found in 23.3% (n=14) of the samples, followed by OXA-48-like, which was found in 15% (n=9) of the samples. The detection of CTX-, CTX-M, KPC, mecA/C, and MREJ, VIM resistant genes were at lower frequencies.
Conclusion: The FilmArray Pneumonia Panel Plus (FA-Pneumo) is a rapid molecular diagnostic tool designed to overcome the limitations of conventional methods by quickly identifying a broad range of pathogens and resistance genes. The capability of FA-Pneumo to rapidly identify both pathogens and key resistance genes provides a significant advantage for clinical decision-making.
期刊介绍：
La Clinica Terapeutica è una rivista di Clinica e Terapia in Medicina e Chirurgia, fondata nel 1951 dal Prof. Mariano Messini (1901-1980), Direttore dell''Istituto di Idrologia Medica dell''Università di Roma “La Sapienza”. La rivista è pubblicata come “periodico bimestrale” dalla Società Editrice Universo, casa editrice fondata nel 1945 dal Comm. Luigi Pellino. La Clinica Terapeutica è indicizzata su MEDLINE, INDEX MEDICUS, EMBASE/Excerpta Medica.
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