纳米气泡水用于有效去除大肠杆菌形成的生物膜。

IF 1.9 4区生物学 Q4 BIOCHEMICAL RESEARCH METHODS

Journal of microbiological methods Pub Date : 2025-09-22 DOI:10.1016/j.mimet.2025.107276

Fumiyuki Kobayashi , Takeru Kawahara , Asako Narai-Kanayama , Sachiko Odake

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引用次数: 0

摘要

研究了不同气体和两种生成方法制备纳米气泡水对大肠杆菌形成的生物膜的去除效果。所有NB水样对大肠杆菌的去除率均显著高于非NB水样。其中，氮（N2） NB水对大肠杆菌的去除效果最大。N2NB水的表面张力最低，而airNB水的表面张力最高。O2NB水中溶解氧（O2）浓度急剧升高，而N2NB和二氧化碳（CO2） NB水中溶解氧（O2）浓度下降。CO2NB水的pH值显著降低，而N2NB、airNB和O2NB水的pH值升高。在喷射式和剪切式两种铌发生器中，对N2NB水的大肠杆菌去除率无显著差异。然而，不同类型的NB发生器的表面张力、pH值和尺寸分布不同。因此，我们发现NB水对大肠杆菌的去除效果不同，且效果随气体类型的不同而不同。

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Nanobubble water for the effective removal of biofilm formed by Escherichia coli

The removal of biofilm (BF) formed by Escherichia coli using nanobubble (NB) water prepared with different gases and two generation methods was investigated. The E. coli BF removal efficiencies of all NB water samples tested were significantly higher than those of the non-NB water. In particular, nitrogen (N₂) NB water exerted the greatest effect on the removal of E. coli BF. The surface tension of N₂NB water was the lowest, although that of airNB water was the highest. The dissolved oxygen (O₂) concentration in the O₂NB water was drastically increased, although that in the N₂NB and carbon dioxide (CO₂) NB water was decreased. The pH of CO₂NB water was significantly decreased, although that of N₂NB, airNB, and O₂NB water was increased. The E coli BF removal efficiencies of N₂NB water were not different in the NB generators between ejector and shearing types. However, the surface tension, pH, and size distribution varied among the NB generator types. Therefore, it was found that NB water removed E. coli BF, and that its efficiencies differed depending on the gas type.

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来源期刊

Journal of microbiological methods 生物-生化研究方法

CiteScore

4.30

自引率

4.50%

发文量

151

审稿时长

29 days

期刊介绍： The Journal of Microbiological Methods publishes scholarly and original articles, notes and review articles. These articles must include novel and/or state-of-the-art methods, or significant improvements to existing methods. Novel and innovative applications of current methods that are validated and useful will also be published. JMM strives for scholarship, innovation and excellence. This demands scientific rigour, the best available methods and technologies, correctly replicated experiments/tests, the inclusion of proper controls, calibrations, and the correct statistical analysis. The presentation of the data must support the interpretation of the method/approach. All aspects of microbiology are covered, except virology. These include agricultural microbiology, applied and environmental microbiology, bioassays, bioinformatics, biotechnology, biochemical microbiology, clinical microbiology, diagnostics, food monitoring and quality control microbiology, microbial genetics and genomics, geomicrobiology, microbiome methods regardless of habitat, high through-put sequencing methods and analysis, microbial pathogenesis and host responses, metabolomics, metagenomics, metaproteomics, microbial ecology and diversity, microbial physiology, microbial ultra-structure, microscopic and imaging methods, molecular microbiology, mycology, novel mathematical microbiology and modelling, parasitology, plant-microbe interactions, protein markers/profiles, proteomics, pyrosequencing, public health microbiology, radioisotopes applied to microbiology, robotics applied to microbiological methods,rumen microbiology, microbiological methods for space missions and extreme environments, sampling methods and samplers, soil and sediment microbiology, transcriptomics, veterinary microbiology, sero-diagnostics and typing/identification.