冯·科萨钙染色程序再述。

IF 1.5 4区 生物学 Q4 CELL BIOLOGY
Sheila Criswell
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引用次数: 0

摘要

对哺乳动物组织中钙沉积最敏感、最常用的染色方法可能是von Kossa染色法,这种染色法使用了一个多世纪。当最初纳入组织病理学实验室石蜡组织切片的日常使用时,该程序要求将组织切片浸泡在5%硝酸银水溶液中,并在阳光直射下放置20-30分钟,使钙沉积呈现黑色。虽然这种有效的方法继续在选定的地点使用,但现代实验室往往无法获得明亮的阳光。本研究通过更换光源、硝酸银浓度和银溶液曝光时间,重新审视了传统的von Kossa染色方法,并确定1%硝酸银溶液与5%硝酸银溶液在明亮阳光下30-60分钟的效果相同。此外,1%硝酸银溶液可以成功地在3.5W发光二极管(LED)日光灯下暴露1小时,通过有色玻璃窗照射1小时,或头顶房间照明2小时,并且染色反应不受1℃至40℃温度的影响。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Von Kossa Calcium Staining Procedure Revisited.

Probably the most sensitive and frequently performed stain for calcium deposits in mammalian tissues is the von Kossa, a stain used for over a century. When originally incorporated into daily use in the histopathology laboratory on paraffin tissue sections, the procedure called for immersion of tissue sections in an aqueous 5% silver nitrate solution and placed in direct sunlight for 20-30 min to make calcium deposits appear black. Although this effective method continues to be used in select locations, the modern laboratory often does not have access to bright sunlight. This study revisited the traditional von Kossa staining method with alternatives in light sources, silver nitrate concentrations, and timing of silver solution exposure and determined that a 1% silver nitrate solution is equally effective as a 5% solution in bright sunlight for 30-60 min. In addition, a 1% silver nitrate solution can be successfully used with exposure to a 3.5W light-emitting diode (LED) daylight lamp for 1 hr, sunlight through a tinted glass window for 1 hr, or overhead room lighting for 2 hr, and the staining reaction is unaffected by temperatures ranging from 1C to 40C.

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来源期刊
CiteScore
6.00
自引率
0.00%
发文量
32
审稿时长
1 months
期刊介绍: Journal of Histochemistry & Cytochemistry (JHC) has been a pre-eminent cell biology journal for over 50 years. Published monthly, JHC offers primary research articles, timely reviews, editorials, and perspectives on the structure and function of cells, tissues, and organs, as well as mechanisms of development, differentiation, and disease. JHC also publishes new developments in microscopy and imaging, especially where imaging techniques complement current genetic, molecular and biochemical investigations of cell and tissue function. JHC offers generous space for articles and recognizing the value of images that reveal molecular, cellular and tissue organization, offers free color to all authors.
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