Lívia Bruni de Souza, Ana Beatriz P Brandão, Fabiana G Ferreira, Vinicius Guzzoni, Raquel C M F Albuquerque, Lys A Mendes, Rodrigo Yokota, Fernando G Porto, Dulce E Casarini, Flavio Aimbire, Nilsa R Damaceno-Rodrigues, Nádia Bertoncello, Karina R Casali, Tatiana S Cunha
{"title":"鼠李糖乳杆菌和罗伊氏乳酸杆菌益生菌调节炎症，减轻慢性应激性肝损伤和大鼠行为。","authors":"Lívia Bruni de Souza, Ana Beatriz P Brandão, Fabiana G Ferreira, Vinicius Guzzoni, Raquel C M F Albuquerque, Lys A Mendes, Rodrigo Yokota, Fernando G Porto, Dulce E Casarini, Flavio Aimbire, Nilsa R Damaceno-Rodrigues, Nádia Bertoncello, Karina R Casali, Tatiana S Cunha","doi":"10.1016/j.tjnut.2025.09.011","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Chronic stress disrupts systemic homeostas and contributes to metabolic, psychiatric, and inflammatory disorders, often involving alterations in gut microbiota. Probiotics such as Lacticaseibacillus rhamnosus (L. rhamnosus) and Limosilactobacillus reuteri (L. reuteri) possess anti-inflammatory properties, but their combined effects on liver health and cognitive function remain underexplored.</p><p><strong>Objective: </strong>Investigate whether supplementation with L. rhamnosus and L. reuteri mitigates inflammation, liver damage, and behavioral changes induced by chronic stress.</p><p><strong>Methods: </strong>Forty-seven male Wistar rats were assigned to four groups: Control (C, n=12), Stress (S, n=12), Control Probiotic (CP, n=11), and Stress Probiotic (SP, n=12). All animals received standard laboratory chow. A combined dose of L. rhamnosus and L. reuteri (1×10<sup>9</sup> CFU/strain/day) was administered by gavage for 8 weeks. Groups S and SP were subjected to a chronic mild unpredictable stress protocol during weeks 3-5, including stressors such as food/water deprivation, restraint, and light/dark cycle inversion. Behavioral, biochemical, inflammatory, hepatic, metabolic, and physiological parameters were assessed.</p><p><strong>Results: </strong>Probiotic supplementation significantly restored body weight gain (SP: 123.5±17.5% vs. S: 98.2±12.2%) and sucrose preference (SP: 19.96±0.31% vs. S: 14.48±4.16%). SP rats showed reduced levels of corticosterone (SP: 65.2±31.2 ng/mL; S: 188±112.6 ng/mL), noradrenaline (SP: 243±163.6 pg/mL; S: 887.6±476 pg/mL), and dopamine (SP: 318.6±169 pg/mL; S: 582.8±271 pg/mL). Supplementation also decreased ALT levels (SP: 131.6±9.4 U/L vs. S: 147.6±7.5 U/L) and hepatocyte nuclear area (SP: 11.35±1.5 μm<sup>2</sup> vs. S: 12.63 ± 1.7 μm<sup>2</sup>). In circulating analyses, probiotics modulated inflammation by reducing IL-1β (SP: 115±43 pg/mg; S: 343±64 pg/mg) and IL-17 (SP: 356.5±93 pg/mg; S: 674±48 pg/mg), while increasing IL-10 levels (SP: 196.5±11 pg/mg; S: 65±28.6 pg/mg) (all p < 0.05).</p><p><strong>Conclusion: </strong>L. rhamnosus and L. reuteri supplementation attenuated the metabolic, hormonal, inflammatory, and behavioral effects of chronic stress and preserved liver integrity in rats.</p>","PeriodicalId":16620,"journal":{"name":"Journal of Nutrition","volume":" ","pages":""},"PeriodicalIF":3.8000,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Lacticaseibacillus rhamnosus and Limosilactobacillus reuteri probiotics modulate inflammation, attenuating chronic stress-induced liver injury and behavior in rats.\",\"authors\":\"Lívia Bruni de Souza, Ana Beatriz P Brandão, Fabiana G Ferreira, Vinicius Guzzoni, Raquel C M F Albuquerque, Lys A Mendes, Rodrigo Yokota, Fernando G Porto, Dulce E Casarini, Flavio Aimbire, Nilsa R Damaceno-Rodrigues, Nádia Bertoncello, Karina R Casali, Tatiana S Cunha\",\"doi\":\"10.1016/j.tjnut.2025.09.011\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Chronic stress disrupts systemic homeostas and contributes to metabolic, psychiatric, and inflammatory disorders, often involving alterations in gut microbiota. Probiotics such as Lacticaseibacillus rhamnosus (L. rhamnosus) and Limosilactobacillus reuteri (L. reuteri) possess anti-inflammatory properties, but their combined effects on liver health and cognitive function remain underexplored.</p><p><strong>Objective: </strong>Investigate whether supplementation with L. rhamnosus and L. reuteri mitigates inflammation, liver damage, and behavioral changes induced by chronic stress.</p><p><strong>Methods: </strong>Forty-seven male Wistar rats were assigned to four groups: Control (C, n=12), Stress (S, n=12), Control Probiotic (CP, n=11), and Stress Probiotic (SP, n=12). All animals received standard laboratory chow. A combined dose of L. rhamnosus and L. reuteri (1×10<sup>9</sup> CFU/strain/day) was administered by gavage for 8 weeks. Groups S and SP were subjected to a chronic mild unpredictable stress protocol during weeks 3-5, including stressors such as food/water deprivation, restraint, and light/dark cycle inversion. Behavioral, biochemical, inflammatory, hepatic, metabolic, and physiological parameters were assessed.</p><p><strong>Results: </strong>Probiotic supplementation significantly restored body weight gain (SP: 123.5±17.5% vs. S: 98.2±12.2%) and sucrose preference (SP: 19.96±0.31% vs. S: 14.48±4.16%). SP rats showed reduced levels of corticosterone (SP: 65.2±31.2 ng/mL; S: 188±112.6 ng/mL), noradrenaline (SP: 243±163.6 pg/mL; S: 887.6±476 pg/mL), and dopamine (SP: 318.6±169 pg/mL; S: 582.8±271 pg/mL). Supplementation also decreased ALT levels (SP: 131.6±9.4 U/L vs. S: 147.6±7.5 U/L) and hepatocyte nuclear area (SP: 11.35±1.5 μm<sup>2</sup> vs. S: 12.63 ± 1.7 μm<sup>2</sup>). In circulating analyses, probiotics modulated inflammation by reducing IL-1β (SP: 115±43 pg/mg; S: 343±64 pg/mg) and IL-17 (SP: 356.5±93 pg/mg; S: 674±48 pg/mg), while increasing IL-10 levels (SP: 196.5±11 pg/mg; S: 65±28.6 pg/mg) (all p < 0.05).</p><p><strong>Conclusion: </strong>L. rhamnosus and L. reuteri supplementation attenuated the metabolic, hormonal, inflammatory, and behavioral effects of chronic stress and preserved liver integrity in rats.</p>\",\"PeriodicalId\":16620,\"journal\":{\"name\":\"Journal of Nutrition\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":3.8000,\"publicationDate\":\"2025-09-22\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Nutrition\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1016/j.tjnut.2025.09.011\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"NUTRITION & DIETETICS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Nutrition","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1016/j.tjnut.2025.09.011","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"NUTRITION & DIETETICS","Score":null,"Total":0}
Lacticaseibacillus rhamnosus and Limosilactobacillus reuteri probiotics modulate inflammation, attenuating chronic stress-induced liver injury and behavior in rats.
Background: Chronic stress disrupts systemic homeostas and contributes to metabolic, psychiatric, and inflammatory disorders, often involving alterations in gut microbiota. Probiotics such as Lacticaseibacillus rhamnosus (L. rhamnosus) and Limosilactobacillus reuteri (L. reuteri) possess anti-inflammatory properties, but their combined effects on liver health and cognitive function remain underexplored.
Objective: Investigate whether supplementation with L. rhamnosus and L. reuteri mitigates inflammation, liver damage, and behavioral changes induced by chronic stress.
Methods: Forty-seven male Wistar rats were assigned to four groups: Control (C, n=12), Stress (S, n=12), Control Probiotic (CP, n=11), and Stress Probiotic (SP, n=12). All animals received standard laboratory chow. A combined dose of L. rhamnosus and L. reuteri (1×109 CFU/strain/day) was administered by gavage for 8 weeks. Groups S and SP were subjected to a chronic mild unpredictable stress protocol during weeks 3-5, including stressors such as food/water deprivation, restraint, and light/dark cycle inversion. Behavioral, biochemical, inflammatory, hepatic, metabolic, and physiological parameters were assessed.
Results: Probiotic supplementation significantly restored body weight gain (SP: 123.5±17.5% vs. S: 98.2±12.2%) and sucrose preference (SP: 19.96±0.31% vs. S: 14.48±4.16%). SP rats showed reduced levels of corticosterone (SP: 65.2±31.2 ng/mL; S: 188±112.6 ng/mL), noradrenaline (SP: 243±163.6 pg/mL; S: 887.6±476 pg/mL), and dopamine (SP: 318.6±169 pg/mL; S: 582.8±271 pg/mL). Supplementation also decreased ALT levels (SP: 131.6±9.4 U/L vs. S: 147.6±7.5 U/L) and hepatocyte nuclear area (SP: 11.35±1.5 μm2 vs. S: 12.63 ± 1.7 μm2). In circulating analyses, probiotics modulated inflammation by reducing IL-1β (SP: 115±43 pg/mg; S: 343±64 pg/mg) and IL-17 (SP: 356.5±93 pg/mg; S: 674±48 pg/mg), while increasing IL-10 levels (SP: 196.5±11 pg/mg; S: 65±28.6 pg/mg) (all p < 0.05).
Conclusion: L. rhamnosus and L. reuteri supplementation attenuated the metabolic, hormonal, inflammatory, and behavioral effects of chronic stress and preserved liver integrity in rats.
期刊介绍：
The Journal of Nutrition (JN/J Nutr) publishes peer-reviewed original research papers covering all aspects of experimental nutrition in humans and other animal species; special articles such as reviews and biographies of prominent nutrition scientists; and issues, opinions, and commentaries on controversial issues in nutrition. Supplements are frequently published to provide extended discussion of topics of special interest.
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