通过共同菌根网络诱导种间系统抗性的转录组学证据。

IF 3.8 2区 生物学 Q2 MYCOLOGY
Yingde Li, Yong Wei, Panpan Shang, Rongchun Zheng, Youlei Shen, Yajie Wang, Lijun Gu, Tingyu Duan
{"title":"通过共同菌根网络诱导种间系统抗性的转录组学证据。","authors":"Yingde Li, Yong Wei, Panpan Shang, Rongchun Zheng, Youlei Shen, Yajie Wang, Lijun Gu, Tingyu Duan","doi":"10.1007/s00572-025-01230-7","DOIUrl":null,"url":null,"abstract":"<p><p>Plants face persistent biotic pressures from pathogens and herbivores, necessitating effective defense mechanisms. Common mycorrhizal networks (CMNs), formed by arbuscular mycorrhizal fungi, facilitate inter-plant defense signal transmission. However, little is known about interspecies signal transmission through CMNs. This study aimed to investigate whether defense signals can be transferred from pathogen-infected white clover (Trifolium repens) to neighboring healthy perennial ryegrass (Lolium perenne) via CMNs and to assess the subsequent activation of disease-resistance responses in ryegrass at the transcriptional level. A dual-chamber experimental setup was used, in which white clover served as the donor plant and perennial ryegrass was the receiver plant. The results showed that pathogen-induced defense signals can be transferred from infected donor clover to healthy ryegrass plants through CMNs, inducing the expression of resistance genes in the neighboring plants, especially TRINITY_DN52759_c0_g1 (disease resistance protein RGA2). Importantly, the upregulated expression of defense genes was closely related to CAT activity. These factors enhance the defense response of neighboring healthy plants against potential pathogens. Although the pathogenic microbe evaluated is non-pathogenic to ryegrass, the signal transmission mechanism may apply to scenarios with shared threats. In addition, we identified gene modules significantly associated with CAT through weighted gene co-expression network analysis and screened key network genes (TRINITY_DN29267_c0_g1, TRINITY_DN37961_c0_g1, TRINITY_DN74958_c0_g2, TRINITY_DN7515_c0_g1, TRINITY_DN7587_c0_g6) in co-expression networks, which potentially play critical roles in defense responses. The findings suggest that CMNs serve as vital conduits for interplant defense signal transfer, facilitating early warning and defense activation against potential pathogens.</p>","PeriodicalId":18965,"journal":{"name":"Mycorrhiza","volume":"35 5","pages":"55"},"PeriodicalIF":3.8000,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Transcriptomic evidence of interspecies systemic resistance induction via common mycorrhizal networks.\",\"authors\":\"Yingde Li, Yong Wei, Panpan Shang, Rongchun Zheng, Youlei Shen, Yajie Wang, Lijun Gu, Tingyu Duan\",\"doi\":\"10.1007/s00572-025-01230-7\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Plants face persistent biotic pressures from pathogens and herbivores, necessitating effective defense mechanisms. Common mycorrhizal networks (CMNs), formed by arbuscular mycorrhizal fungi, facilitate inter-plant defense signal transmission. However, little is known about interspecies signal transmission through CMNs. This study aimed to investigate whether defense signals can be transferred from pathogen-infected white clover (Trifolium repens) to neighboring healthy perennial ryegrass (Lolium perenne) via CMNs and to assess the subsequent activation of disease-resistance responses in ryegrass at the transcriptional level. A dual-chamber experimental setup was used, in which white clover served as the donor plant and perennial ryegrass was the receiver plant. The results showed that pathogen-induced defense signals can be transferred from infected donor clover to healthy ryegrass plants through CMNs, inducing the expression of resistance genes in the neighboring plants, especially TRINITY_DN52759_c0_g1 (disease resistance protein RGA2). Importantly, the upregulated expression of defense genes was closely related to CAT activity. These factors enhance the defense response of neighboring healthy plants against potential pathogens. Although the pathogenic microbe evaluated is non-pathogenic to ryegrass, the signal transmission mechanism may apply to scenarios with shared threats. In addition, we identified gene modules significantly associated with CAT through weighted gene co-expression network analysis and screened key network genes (TRINITY_DN29267_c0_g1, TRINITY_DN37961_c0_g1, TRINITY_DN74958_c0_g2, TRINITY_DN7515_c0_g1, TRINITY_DN7587_c0_g6) in co-expression networks, which potentially play critical roles in defense responses. The findings suggest that CMNs serve as vital conduits for interplant defense signal transfer, facilitating early warning and defense activation against potential pathogens.</p>\",\"PeriodicalId\":18965,\"journal\":{\"name\":\"Mycorrhiza\",\"volume\":\"35 5\",\"pages\":\"55\"},\"PeriodicalIF\":3.8000,\"publicationDate\":\"2025-09-25\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Mycorrhiza\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1007/s00572-025-01230-7\",\"RegionNum\":2,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"MYCOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Mycorrhiza","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s00572-025-01230-7","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MYCOLOGY","Score":null,"Total":0}
引用次数: 0

摘要

植物面临着来自病原体和食草动物的持续生物压力,需要有效的防御机制。共同菌根网络(CMNs)由丛枝菌根真菌形成,促进植物间防御信号的传递。然而,通过CMNs的物种间信号传输知之甚少。本研究旨在探讨感染病原菌的白三叶草(Trifolium repens)的防御信号是否可以通过CMNs传递给邻近的健康多年生黑麦草(Lolium perenne),并在转录水平上评估黑麦草抗病反应的激活情况。采用双室实验装置,以白三叶为供体植物,多年生黑麦草为受体植物。结果表明,病原菌诱导的防御信号可通过CMNs从感染的供体三叶草转移到健康的黑麦草植株上,诱导邻近植株中抗病基因的表达,尤其是trity_dn52759_c0_g1(抗病蛋白RGA2)的表达。重要的是,防御基因的表达上调与CAT活性密切相关。这些因素增强了邻近健康植物对潜在病原体的防御反应。虽然评估的病原微生物对黑麦草无致病性,但信号传递机制可能适用于具有共同威胁的情况。此外,我们通过加权基因共表达网络分析发现了与CAT显著相关的基因模块,并筛选了共表达网络中的关键网络基因(TRINITY_DN29267_c0_g1、TRINITY_DN37961_c0_g1、TRINITY_DN74958_c0_g2、TRINITY_DN7515_c0_g1、TRINITY_DN7587_c0_g6),这些基因可能在防御反应中发挥关键作用。研究结果表明,CMNs是植物间防御信号传递的重要通道,促进了对潜在病原体的早期预警和防御激活。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Transcriptomic evidence of interspecies systemic resistance induction via common mycorrhizal networks.

Plants face persistent biotic pressures from pathogens and herbivores, necessitating effective defense mechanisms. Common mycorrhizal networks (CMNs), formed by arbuscular mycorrhizal fungi, facilitate inter-plant defense signal transmission. However, little is known about interspecies signal transmission through CMNs. This study aimed to investigate whether defense signals can be transferred from pathogen-infected white clover (Trifolium repens) to neighboring healthy perennial ryegrass (Lolium perenne) via CMNs and to assess the subsequent activation of disease-resistance responses in ryegrass at the transcriptional level. A dual-chamber experimental setup was used, in which white clover served as the donor plant and perennial ryegrass was the receiver plant. The results showed that pathogen-induced defense signals can be transferred from infected donor clover to healthy ryegrass plants through CMNs, inducing the expression of resistance genes in the neighboring plants, especially TRINITY_DN52759_c0_g1 (disease resistance protein RGA2). Importantly, the upregulated expression of defense genes was closely related to CAT activity. These factors enhance the defense response of neighboring healthy plants against potential pathogens. Although the pathogenic microbe evaluated is non-pathogenic to ryegrass, the signal transmission mechanism may apply to scenarios with shared threats. In addition, we identified gene modules significantly associated with CAT through weighted gene co-expression network analysis and screened key network genes (TRINITY_DN29267_c0_g1, TRINITY_DN37961_c0_g1, TRINITY_DN74958_c0_g2, TRINITY_DN7515_c0_g1, TRINITY_DN7587_c0_g6) in co-expression networks, which potentially play critical roles in defense responses. The findings suggest that CMNs serve as vital conduits for interplant defense signal transfer, facilitating early warning and defense activation against potential pathogens.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Mycorrhiza
Mycorrhiza 生物-真菌学
CiteScore
8.20
自引率
2.60%
发文量
40
审稿时长
6-12 weeks
期刊介绍: Mycorrhiza is an international journal devoted to research into mycorrhizas - the widest symbioses in nature, involving plants and a range of soil fungi world-wide. The scope of Mycorrhiza covers all aspects of research into mycorrhizas, including molecular biology of the plants and fungi, fungal systematics, development and structure of mycorrhizas, and effects on plant physiology, productivity, reproduction and disease resistance. The scope also includes interactions between mycorrhizal fungi and other soil organisms and effects of mycorrhizas on plant biodiversity and ecosystem structure. Mycorrhiza contains original papers, short notes and review articles, along with commentaries and news items. It forms a platform for new concepts and discussions, and is a basis for a truly international forum of mycorrhizologists from all over the world.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信