{"title":"评估肠道微生物群、炎症标志物和结直肠癌之间的关系。","authors":"Yan Cui","doi":"10.3389/fcimb.2025.1604651","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>This study aims to evaluate the correlation between inflammation and gut microbiota characteristics in patients with colorectal cancer (CRC) through a retrospective study.</p><p><strong>Methods: </strong>This cross-sectional, non-interventional study included a total of 200 subjects, of which 150 were colorectal cancer (CRC) patients and 50 were healthy individuals. The study retrospectively reviewed hospital and laboratory archives and records from 2015 to 2020. Gut microbiota was analyzed using 16S rRNA sequencing. Inflammatory markers, including C-reactive protein (CRP), interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-α), and interleukin-beta (IL-1β), were measured using enzyme-linked immunosorbent assay (ELISA). Logistic regression analysis was used to evaluate the relationship between gut microbiota, inflammatory markers, and CRC.</p><p><strong>Results: </strong>Subjects in the colorectal cancer (CRC) group exhibited a higher proportion of Firmicutes (47.2% <i>vs</i>. 39.0%). Levels of both Firmicutes and Proteobacteria were significantly elevated in the CRC group, while Bacteroidetes levels were lower. Additionally, elevated levels of inflammatory markers were observed in the CRC group, including C-reactive protein (CRP: 9.8 mg/L <i>vs</i>. 4.1 mg/L, P<0.01), interleukin-6 (IL-6: 14.5 pg/mL <i>vs</i>. 6.2 pg/mL, P<0.01), tumor necrosis factor-alpha (TNF-α: 9.2 pg/mL <i>vs</i>. 4.3 pg/mL, P<0.01), and interleukin-1β (IL-1β: 5.8 pg/mL <i>vs</i>. 3.6 pg/mL, P<0.01). Multivariate analysis showed that higher levels of Firmicutes (OR=2.5, 95% CI: 1.4-4.5, P<0.01) and Proteobacteria (OR=2.8, 95% CI: 1.6-4.9, P<0.01) were significantly associated with an increased risk of CRC. Elevated levels of CRP (OR=3.1, 95% CI: 1.8-5.3, P<0.01) and IL-6 (OR=3.4, 95% CI: 2.0-5.8, P<0.01) were also significantly associated with an increased risk of CRC.</p><p><strong>Conclusion: </strong>There is a significant correlation between changes in gut microbiota composition and cytokine levels with the risk of colorectal cancer (CRC).</p>","PeriodicalId":12458,"journal":{"name":"Frontiers in Cellular and Infection Microbiology","volume":"15 ","pages":"1604651"},"PeriodicalIF":4.8000,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12455238/pdf/","citationCount":"0","resultStr":"{\"title\":\"Assessment of the relationship between gut microbiota, inflammatory markers, and colorectal cancer.\",\"authors\":\"Yan Cui\",\"doi\":\"10.3389/fcimb.2025.1604651\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objective: </strong>This study aims to evaluate the correlation between inflammation and gut microbiota characteristics in patients with colorectal cancer (CRC) through a retrospective study.</p><p><strong>Methods: </strong>This cross-sectional, non-interventional study included a total of 200 subjects, of which 150 were colorectal cancer (CRC) patients and 50 were healthy individuals. The study retrospectively reviewed hospital and laboratory archives and records from 2015 to 2020. Gut microbiota was analyzed using 16S rRNA sequencing. Inflammatory markers, including C-reactive protein (CRP), interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-α), and interleukin-beta (IL-1β), were measured using enzyme-linked immunosorbent assay (ELISA). Logistic regression analysis was used to evaluate the relationship between gut microbiota, inflammatory markers, and CRC.</p><p><strong>Results: </strong>Subjects in the colorectal cancer (CRC) group exhibited a higher proportion of Firmicutes (47.2% <i>vs</i>. 39.0%). Levels of both Firmicutes and Proteobacteria were significantly elevated in the CRC group, while Bacteroidetes levels were lower. Additionally, elevated levels of inflammatory markers were observed in the CRC group, including C-reactive protein (CRP: 9.8 mg/L <i>vs</i>. 4.1 mg/L, P<0.01), interleukin-6 (IL-6: 14.5 pg/mL <i>vs</i>. 6.2 pg/mL, P<0.01), tumor necrosis factor-alpha (TNF-α: 9.2 pg/mL <i>vs</i>. 4.3 pg/mL, P<0.01), and interleukin-1β (IL-1β: 5.8 pg/mL <i>vs</i>. 3.6 pg/mL, P<0.01). Multivariate analysis showed that higher levels of Firmicutes (OR=2.5, 95% CI: 1.4-4.5, P<0.01) and Proteobacteria (OR=2.8, 95% CI: 1.6-4.9, P<0.01) were significantly associated with an increased risk of CRC. Elevated levels of CRP (OR=3.1, 95% CI: 1.8-5.3, P<0.01) and IL-6 (OR=3.4, 95% CI: 2.0-5.8, P<0.01) were also significantly associated with an increased risk of CRC.</p><p><strong>Conclusion: </strong>There is a significant correlation between changes in gut microbiota composition and cytokine levels with the risk of colorectal cancer (CRC).</p>\",\"PeriodicalId\":12458,\"journal\":{\"name\":\"Frontiers in Cellular and Infection Microbiology\",\"volume\":\"15 \",\"pages\":\"1604651\"},\"PeriodicalIF\":4.8000,\"publicationDate\":\"2025-09-09\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12455238/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Frontiers in Cellular and Infection Microbiology\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.3389/fcimb.2025.1604651\",\"RegionNum\":2,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q2\",\"JCRName\":\"IMMUNOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Frontiers in Cellular and Infection Microbiology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.3389/fcimb.2025.1604651","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/1/1 0:00:00","PubModel":"eCollection","JCR":"Q2","JCRName":"IMMUNOLOGY","Score":null,"Total":0}
Assessment of the relationship between gut microbiota, inflammatory markers, and colorectal cancer.
Objective: This study aims to evaluate the correlation between inflammation and gut microbiota characteristics in patients with colorectal cancer (CRC) through a retrospective study.
Methods: This cross-sectional, non-interventional study included a total of 200 subjects, of which 150 were colorectal cancer (CRC) patients and 50 were healthy individuals. The study retrospectively reviewed hospital and laboratory archives and records from 2015 to 2020. Gut microbiota was analyzed using 16S rRNA sequencing. Inflammatory markers, including C-reactive protein (CRP), interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-α), and interleukin-beta (IL-1β), were measured using enzyme-linked immunosorbent assay (ELISA). Logistic regression analysis was used to evaluate the relationship between gut microbiota, inflammatory markers, and CRC.
Results: Subjects in the colorectal cancer (CRC) group exhibited a higher proportion of Firmicutes (47.2% vs. 39.0%). Levels of both Firmicutes and Proteobacteria were significantly elevated in the CRC group, while Bacteroidetes levels were lower. Additionally, elevated levels of inflammatory markers were observed in the CRC group, including C-reactive protein (CRP: 9.8 mg/L vs. 4.1 mg/L, P<0.01), interleukin-6 (IL-6: 14.5 pg/mL vs. 6.2 pg/mL, P<0.01), tumor necrosis factor-alpha (TNF-α: 9.2 pg/mL vs. 4.3 pg/mL, P<0.01), and interleukin-1β (IL-1β: 5.8 pg/mL vs. 3.6 pg/mL, P<0.01). Multivariate analysis showed that higher levels of Firmicutes (OR=2.5, 95% CI: 1.4-4.5, P<0.01) and Proteobacteria (OR=2.8, 95% CI: 1.6-4.9, P<0.01) were significantly associated with an increased risk of CRC. Elevated levels of CRP (OR=3.1, 95% CI: 1.8-5.3, P<0.01) and IL-6 (OR=3.4, 95% CI: 2.0-5.8, P<0.01) were also significantly associated with an increased risk of CRC.
Conclusion: There is a significant correlation between changes in gut microbiota composition and cytokine levels with the risk of colorectal cancer (CRC).
期刊介绍：
Frontiers in Cellular and Infection Microbiology is a leading specialty journal, publishing rigorously peer-reviewed research across all pathogenic microorganisms and their interaction with their hosts. Chief Editor Yousef Abu Kwaik, University of Louisville is supported by an outstanding Editorial Board of international experts. This multidisciplinary open-access journal is at the forefront of disseminating and communicating scientific knowledge and impactful discoveries to researchers, academics, clinicians and the public worldwide.
Frontiers in Cellular and Infection Microbiology includes research on bacteria, fungi, parasites, viruses, endosymbionts, prions and all microbial pathogens as well as the microbiota and its effect on health and disease in various hosts. The research approaches include molecular microbiology, cellular microbiology, gene regulation, proteomics, signal transduction, pathogenic evolution, genomics, structural biology, and virulence factors as well as model hosts. Areas of research to counteract infectious agents by the host include the host innate and adaptive immune responses as well as metabolic restrictions to various pathogenic microorganisms, vaccine design and development against various pathogenic microorganisms, and the mechanisms of antibiotic resistance and its countermeasures.
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