利用blaZ qPCR直接在血培养上检测金黄色葡萄球菌青霉素基因型敏感性，结果显示金黄色葡萄球菌基因型与表型高度一致。

IF 3 3区医学 Q2 INFECTIOUS DISEASES

European Journal of Clinical Microbiology & Infectious Diseases Pub Date : 2025-09-25 DOI:10.1007/s10096-025-05272-9

Tobias Müller Vang, Barbara Juliane Holzknecht, Stephen Strunge Nilsson, Morten Hoppe, Michael Pedersen, Karen Angeliki Krogfelt, Helle Brander Eriksen

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引用次数: 0

摘要

研究了金黄色葡萄球菌血培养对青霉素敏感性的基因和表型。在阳性血培养瓶上直接进行blaZ基因的qPCR检测，并与常规的圆盘扩散法和三叶草试验结果进行比较。其中blaZ阳性100支，blaZ阴性101支。基因型和表型型青霉素敏感性完全一致，但一株青霉素耐药株仅呈弱表型阳性。

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Staphylococcus aureus genotypic penicillin susceptibility using a blaZ qPCR directly on blood cultures shows high concordance with phenotype.

Staphylococcus aureus blood cultures were investigated geno- and phenotypically for penicillin susceptibility. A qPCR method detecting the blaZ gene was performed directly on positive blood culture vials and compared with routine disk diffusion results supplemented with the cloverleaf test. Among the collected vials, 100 were blaZ positive and 101 were blaZ negative. Full concordance was observed between genotypical and phenotypical penicillin susceptibility, however one penicillin resistant isolate was only weakly phenotypically positive.

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来源期刊

European Journal of Clinical Microbiology & Infectious Diseases 医学-传染病学

CiteScore

10.40

自引率

2.20%

发文量

138

审稿时长

1 months

期刊介绍： EJCMID is an interdisciplinary journal devoted to the publication of communications on infectious diseases of bacterial, viral and parasitic origin.