关节镜手术中不同盐水悬浮液高度对损伤关节软骨的影响。

IF 2.7 4区 医学 Q1 ORTHOPEDICS
Zeyuan Gao, Yuxin Huang, Simin Kong, Wenqiang Yan, Shuai Yang, Fengyi Hu, Jin Cheng, Xiaoqing Hu, Weili Shi
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引用次数: 0

摘要

目的在关节镜手术中,通常将素悬吊在一定高度以保持手术视野的清晰。生理盐水对软骨的影响已被广泛研究;然而,悬浮在不同高度的盐水溶液所产生的压力对损伤软骨的影响在很大程度上是未知的。本研究探讨了悬浮液高度依赖的细胞反应和损伤软骨组织。方法从猪膝关节处取下半软骨外植体,垂直切开后在4个高度(80/105/130/155 cm)浸泡或用生理盐水冲洗2小时。然后将外植体转移到软骨培养基中培养5天。随后用共聚焦成像评估软骨细胞活力。通过促凋亡和促炎基因的表达水平来评估细胞反应。通过促炎细胞因子和细胞外基质分泌组分析及组织学检查评估组织损伤。结果灌洗加重了软骨浅区切口软骨细胞死亡,80 cm、105 cm、130 cm处软骨细胞轻度改变,155 cm处软骨细胞严重损伤。同样,灌水高度为80 ~ 130 cm的外植体,BAX、BCL2、IL-6、NOS2的基因表达以及GAG、IL-6、NO的释放变化也相对较小。结论本研究证明了灌洗对损伤软骨表面的损伤作用。悬浮高度在80厘米到130厘米之间,导致软骨损伤比较轻微。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The Effect of Different Saline Suspension Height on Injured Articular Cartilage During Arthroscopic Surgery.

ObjectiveSaline is typically suspended at a certain height to maintain a clear surgical field in arthroscopic surgery. The effects of saline on cartilage have been extensively studied; however, the impact of the pressure generated by saline solution suspended at different heights on injured cartilage is largely unknown. This study investigates suspension-height-dependent cellular responses and tissue damage in traumatized cartilage.MethodsOsteochondral explants were harvested from porcine stifle joints, then were cut perpendicularly before immersed or irrigated for 2 hours with saline at 4 heights (80/105/130/155 cm). The explants were then transferred to and cultured in chondrogenic medium for 5 days. Chondrocytes viability was subsequently assessed with confocal imaging. Cell response was assessed with expression levels of proapoptosis and proinflammatory genes. Tissue damage was evaluated by secretome analysis of proinflammatory cytokines and extracellular matrix and histological test.ResultsIrrigation exacerbated cut-induced chondrocytes death in superficial zone of cartilage, with mild change on 80 cm, 105 cm, 130 cm and severe damage on 155 cm. Similarly, explants that underwent irrigation with heights of 80 cm to 130 cm exhibited relatively slighter change of gene expression of BAX, BCL2, IL-6 and NOS2 and release of GAG, IL-6 and NO to a comparable extent.ConclusionThis study provides evidence of damaging effects of irrigation on injured cartilage surface. Suspension heights of 80 cm to 130 cm led to comparable minor cartilage damage.

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来源期刊
CARTILAGE
CARTILAGE ORTHOPEDICS-
CiteScore
6.90
自引率
7.10%
发文量
80
期刊介绍: CARTILAGE publishes articles related to the musculoskeletal system with particular attention to cartilage repair, development, function, degeneration, transplantation, and rehabilitation. The journal is a forum for the exchange of ideas for the many types of researchers and clinicians involved in cartilage biology and repair. A primary objective of CARTILAGE is to foster the cross-fertilization of the findings between clinical and basic sciences throughout the various disciplines involved in cartilage repair. The journal publishes full length original manuscripts on all types of cartilage including articular, nasal, auricular, tracheal/bronchial, and intervertebral disc fibrocartilage. Manuscripts on clinical and laboratory research are welcome. Review articles, editorials, and letters are also encouraged. The ICRS envisages CARTILAGE as a forum for the exchange of knowledge among clinicians, scientists, patients, and researchers. The International Cartilage Repair Society (ICRS) is dedicated to promotion, encouragement, and distribution of fundamental and applied research of cartilage in order to permit a better knowledge of function and dysfunction of articular cartilage and its repair.
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