羊水中的蛋白质成分可增强角质形成细胞分化相关蛋白的表达。

IF 1.3 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
Tokuji Tsuji, Mao Ohashi, Rikuto Imai, Yusuke Kawaguchi, Hisateru Yamaguchi, Shuichi Matsuyama, Sho Nakamura, Satoshi Ohkura, Kiyotaka Hitomi
{"title":"羊水中的蛋白质成分可增强角质形成细胞分化相关蛋白的表达。","authors":"Tokuji Tsuji, Mao Ohashi, Rikuto Imai, Yusuke Kawaguchi, Hisateru Yamaguchi, Shuichi Matsuyama, Sho Nakamura, Satoshi Ohkura, Kiyotaka Hitomi","doi":"10.1093/bbb/zbaf138","DOIUrl":null,"url":null,"abstract":"<p><p>Amniotic fluid (AF) constitutes a dynamic environment containing diverse bioactive molecules derived from both maternal and fetal sources that support fetal development. As the fetus develops in continuous contact with AF, it is plausible that AF influences the formation of the skin epidermis. However, the mechanisms through which AF promotes keratinocyte differentiation remain largely unclear. Here, we showed that goat AF enhanced the expression of key functional proteins involved in epidermal barrier formation, including small proline-rich proteins, loricrin, and transglutaminase. We further obtained the bioactive fractions that promote the expression of these differentiation-related proteins through multistep protein fractionation via column chromatography. Proteomic analysis subsequently revealed 291 candidate proteins, including 85 distinct extracellular proteins, primarily grouped into calcium-binding proteins, proteases and their regulators, extracellular matrix components, and signaling molecules. Collectively, these results suggest that proteins secreted or released into AF contribute to establishing a microenvironment conducive to epidermal differentiation.</p>","PeriodicalId":9175,"journal":{"name":"Bioscience, Biotechnology, and Biochemistry","volume":" ","pages":""},"PeriodicalIF":1.3000,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Proteinaceous components in goat amniotic fluid enhance the expression of keratinocyte differentiation-related proteins.\",\"authors\":\"Tokuji Tsuji, Mao Ohashi, Rikuto Imai, Yusuke Kawaguchi, Hisateru Yamaguchi, Shuichi Matsuyama, Sho Nakamura, Satoshi Ohkura, Kiyotaka Hitomi\",\"doi\":\"10.1093/bbb/zbaf138\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Amniotic fluid (AF) constitutes a dynamic environment containing diverse bioactive molecules derived from both maternal and fetal sources that support fetal development. As the fetus develops in continuous contact with AF, it is plausible that AF influences the formation of the skin epidermis. However, the mechanisms through which AF promotes keratinocyte differentiation remain largely unclear. Here, we showed that goat AF enhanced the expression of key functional proteins involved in epidermal barrier formation, including small proline-rich proteins, loricrin, and transglutaminase. We further obtained the bioactive fractions that promote the expression of these differentiation-related proteins through multistep protein fractionation via column chromatography. Proteomic analysis subsequently revealed 291 candidate proteins, including 85 distinct extracellular proteins, primarily grouped into calcium-binding proteins, proteases and their regulators, extracellular matrix components, and signaling molecules. Collectively, these results suggest that proteins secreted or released into AF contribute to establishing a microenvironment conducive to epidermal differentiation.</p>\",\"PeriodicalId\":9175,\"journal\":{\"name\":\"Bioscience, Biotechnology, and Biochemistry\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":1.3000,\"publicationDate\":\"2025-09-25\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Bioscience, Biotechnology, and Biochemistry\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://doi.org/10.1093/bbb/zbaf138\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bioscience, Biotechnology, and Biochemistry","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1093/bbb/zbaf138","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0

摘要

羊水(AF)是一种动态环境,含有来自母体和胎儿的多种生物活性分子,支持胎儿发育。胎儿在持续接触房颤的情况下发育,房颤可能影响皮肤表皮的形成。然而,AF促进角质细胞分化的机制在很大程度上仍不清楚。在这里,我们发现山羊AF增强了参与表皮屏障形成的关键功能蛋白的表达,包括富含脯氨酸的小蛋白、loricrin和转谷氨酰胺酶。我们进一步通过柱层析的多步骤蛋白质分离获得了促进这些分化相关蛋白表达的生物活性组分。蛋白质组学分析随后揭示了291种候选蛋白,包括85种不同的细胞外蛋白,主要分为钙结合蛋白、蛋白酶及其调节因子、细胞外基质成分和信号分子。总之,这些结果表明,分泌或释放到AF中的蛋白质有助于建立有利于表皮分化的微环境。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Proteinaceous components in goat amniotic fluid enhance the expression of keratinocyte differentiation-related proteins.

Amniotic fluid (AF) constitutes a dynamic environment containing diverse bioactive molecules derived from both maternal and fetal sources that support fetal development. As the fetus develops in continuous contact with AF, it is plausible that AF influences the formation of the skin epidermis. However, the mechanisms through which AF promotes keratinocyte differentiation remain largely unclear. Here, we showed that goat AF enhanced the expression of key functional proteins involved in epidermal barrier formation, including small proline-rich proteins, loricrin, and transglutaminase. We further obtained the bioactive fractions that promote the expression of these differentiation-related proteins through multistep protein fractionation via column chromatography. Proteomic analysis subsequently revealed 291 candidate proteins, including 85 distinct extracellular proteins, primarily grouped into calcium-binding proteins, proteases and their regulators, extracellular matrix components, and signaling molecules. Collectively, these results suggest that proteins secreted or released into AF contribute to establishing a microenvironment conducive to epidermal differentiation.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Bioscience, Biotechnology, and Biochemistry
Bioscience, Biotechnology, and Biochemistry 生物-生化与分子生物学
CiteScore
3.50
自引率
0.00%
发文量
183
审稿时长
1 months
期刊介绍: Bioscience, Biotechnology, and Biochemistry publishes high-quality papers providing chemical and biological analyses of vital phenomena exhibited by animals, plants, and microorganisms, the chemical structures and functions of their products, and related matters. The Journal plays a major role in communicating to a global audience outstanding basic and applied research in all fields subsumed by the Japan Society for Bioscience, Biotechnology, and Agrochemistry (JSBBA).
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信