A N Kazimirskii, J M Salmasi, G V Poryadin, M I Panina, A E Kim, A G Chakhalian
{"title":"转化和肿瘤细胞在体外自发产生含dna的细胞外结构。","authors":"A N Kazimirskii, J M Salmasi, G V Poryadin, M I Panina, A E Kim, A G Chakhalian","doi":"10.1007/s10517-025-06489-6","DOIUrl":null,"url":null,"abstract":"<p><p>The parameters of DNA-containing extracellular structures formed by immortalized fibroblasts and HCT116 adenocarcinoma cells during cultivation were studied. Fluorescence microscopy with SYBR Green dye was used to visualize DNA-containing extracellular structures. In contrast to intact neutrophils, immortalized fibroblasts and adenocarcinoma cells spontaneously formed DNA-containing extracellular structures during incubation. The process begins with the release of a single DNA-containing strand from the cell immediately after the cells are fixed to the substrate (1 h) and ends after 3 h with the formation of DNA-containing extracellular structures in the form of bundles of parallel fibers. These findings indicate active nature of the secretion of DNA-containing structures by immortalized fibroblasts and HCT116 adenocarcinoma cells.</p>","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":" ","pages":"359-362"},"PeriodicalIF":0.6000,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Transformed and Tumor Cells Spontaneously Produce DNA-Containing Extracellular Structures In Vitro.\",\"authors\":\"A N Kazimirskii, J M Salmasi, G V Poryadin, M I Panina, A E Kim, A G Chakhalian\",\"doi\":\"10.1007/s10517-025-06489-6\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>The parameters of DNA-containing extracellular structures formed by immortalized fibroblasts and HCT116 adenocarcinoma cells during cultivation were studied. Fluorescence microscopy with SYBR Green dye was used to visualize DNA-containing extracellular structures. In contrast to intact neutrophils, immortalized fibroblasts and adenocarcinoma cells spontaneously formed DNA-containing extracellular structures during incubation. The process begins with the release of a single DNA-containing strand from the cell immediately after the cells are fixed to the substrate (1 h) and ends after 3 h with the formation of DNA-containing extracellular structures in the form of bundles of parallel fibers. These findings indicate active nature of the secretion of DNA-containing structures by immortalized fibroblasts and HCT116 adenocarcinoma cells.</p>\",\"PeriodicalId\":9331,\"journal\":{\"name\":\"Bulletin of Experimental Biology and Medicine\",\"volume\":\" \",\"pages\":\"359-362\"},\"PeriodicalIF\":0.6000,\"publicationDate\":\"2025-07-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Bulletin of Experimental Biology and Medicine\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1007/s10517-025-06489-6\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/9/25 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q4\",\"JCRName\":\"MEDICINE, RESEARCH & EXPERIMENTAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bulletin of Experimental Biology and Medicine","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1007/s10517-025-06489-6","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/9/25 0:00:00","PubModel":"Epub","JCR":"Q4","JCRName":"MEDICINE, RESEARCH & EXPERIMENTAL","Score":null,"Total":0}
Transformed and Tumor Cells Spontaneously Produce DNA-Containing Extracellular Structures In Vitro.
The parameters of DNA-containing extracellular structures formed by immortalized fibroblasts and HCT116 adenocarcinoma cells during cultivation were studied. Fluorescence microscopy with SYBR Green dye was used to visualize DNA-containing extracellular structures. In contrast to intact neutrophils, immortalized fibroblasts and adenocarcinoma cells spontaneously formed DNA-containing extracellular structures during incubation. The process begins with the release of a single DNA-containing strand from the cell immediately after the cells are fixed to the substrate (1 h) and ends after 3 h with the formation of DNA-containing extracellular structures in the form of bundles of parallel fibers. These findings indicate active nature of the secretion of DNA-containing structures by immortalized fibroblasts and HCT116 adenocarcinoma cells.
期刊介绍：
Bulletin of Experimental Biology and Medicine presents original peer reviewed research papers and brief reports on priority new research results in physiology, biochemistry, biophysics, pharmacology, immunology, microbiology, genetics, oncology, etc. Novel trends in science are covered in new sections of the journal - Biogerontology and Human Ecology - that first appeared in 2005.
World scientific interest in stem cells prompted inclusion into Bulletin of Experimental Biology and Medicine a quarterly scientific journal Cell Technologies in Biology and Medicine (a new Russian Academy of Medical Sciences publication since 2005). It publishes only original papers from the leading research institutions on molecular biology of stem and progenitor cells, stem cell as the basis of gene therapy, molecular language of cell-to-cell communication, cytokines, chemokines, growth and other factors, pilot projects on clinical use of stem and progenitor cells.
The Russian Volume Year is published in English from April.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
