snoRNP RRP9通过SQSTM1促进前列腺癌细胞增殖和迁移

IF 2.6 3区 生物学 Q3 MATERIALS SCIENCE, BIOMATERIALS
Butang Li, Lihui Shen, Hui Huang, Kai Shen, Xiaorong Wu, Chenfei Chi, Jiahua Pan
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引用次数: 0

摘要

小核仁rna (snoRNAs)-60-300核苷酸非编码rna与癌症患者的不良临床结果相关。然而,关于snoRNAs和相关小核核糖核蛋白(snoRNPs)在前列腺癌(PCa)中的作用的信息仍然很少。本文研究了snoRNP U3 snorna相互作用蛋白2 (RRP9)在PCa发病机制中的作用。在PCa细胞系DU-145和PC-3中,使用三种不同shrna的组合来敲低RRP9。通过将细胞植入96孔板并每日监测来评估细胞增殖。通过划痕和Transwell试验评估细胞迁移。采用FLAG-RRP9 pull-down、MALDI-TOF/TOF和共免疫沉淀法鉴定DU-145细胞中的RRP9结合伙伴。RRP9敲低后,体外细胞增殖和迁移以及体内肿瘤生长均受到抑制。Pull-down和MALDI-TOF/TOF分析确定了5个可能的RRP9结合伙伴,共同免疫沉淀验证了RRP9与支架中心蛋白Sequestome-1 (SQSTM1, p62)相互作用。有趣的是,SQSTM1过表达恢复了RRP9敲低的抗生长/迁移作用。这项研究揭示了RRP9-SQSTM1轴在PCa细胞中的一个新的致癌作用。RRP9是一种与SQSTM1结合的snoRNP,从而促进PCa细胞的增殖和迁移。靶向RRP9-SQSTM1轴可能是一种可行的前列腺癌治疗策略。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
snoRNP RRP9 Promotes Prostate Cancer Cell Proliferation and Migration via SQSTM1.

Small nucleolar RNAs (snoRNAs)-60-300 nucleotide non-coding RNAs are associated with adverse clinical outcomes in cancer patients. However, information on the role of snoRNAs and associated small nuclear ribonucleoprotein (snoRNPs) in prostate cancer (PCa) remains scarce. Here, the contribution of the snoRNP U3 snoRNA-interacting protein 2 (RRP9) in PCa pathogenesis is investigated. A combination of three different shRNAs is employed to knockdown RRP9 in the PCa cell lines DU-145 and PC-3. Cell proliferation is evaluated by seeding cells into a 96-well plates and monitoring daily. Cell migration is evaluated by scratch and Transwell assays. FLAG-RRP9 pull-down, MALDI-TOF/TOF, and co-immunoprecipitation assays are conducted to identify RRP9 binding partners in DU-145 cells. In vitro cell proliferation and migration, as well as in vivo tumor growth, are suppressed following RRP9 knockdown. Pull-down and MALDI-TOF/TOF analyses identified five putative RRP9 binding partners, and co-immunoprecipitation validated that RRP9 interacts with the scaffolding hub protein Sequestome-1 (SQSTM1, p62). Interestingly, SQSTM1 overexpression rescued the anti-growth/migration effects of RRP9 knockdown. This study unveiled a novel oncogenic role for the RRP9-SQSTM1 axis in PCa cells. RRP9 is a snoRNP that binds to SQSTM1, thereby promoting PCa cell proliferation and migration. Targeting the RRP9-SQSTM1 axis may be a viable therapeutic strategy for PCa.

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来源期刊
Advanced biology
Advanced biology Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)
CiteScore
6.60
自引率
0.00%
发文量
130
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