{"title":"细胞外囊泡的有效分离纯化方法。","authors":"Taewoon Kim, Jong Wook Hong, Luke P. Lee","doi":"10.1186/s40580-025-00509-x","DOIUrl":null,"url":null,"abstract":"<div><p>Living cells produce nanometer scales of extracellular vesicles (EVs) that have attracted considerable interest due to their transformative effects on diagnostics and therapies for cancer and other diseases. While significant advancements have been made in grasping the physical and chemical foundations of separation techniques for EVs, challenges must be overcome to ensure effective EV purification for diverse life sciences and clinical applications. This review highlights the most significant developments in efficient isolation and purification methods for EVs in transformative medicine. We examine the basic structure of exosomes and how to obtain specimens containing exosomes and EVs from various body fluids. We investigate the principles of physical, chemical, and biological isolation methods of EVs. We systematically evaluate different designs of microfluidics-based EV purification methods. We provide a comprehensive overview of the applications of exosomes in the life sciences and medicine. The precise engineering of EV isolation and purification generates a high yield and purity, offering practical solutions for translational medicine.</p><h3>Graphical Abstract</h3>\n<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":712,"journal":{"name":"Nano Convergence","volume":"12 1","pages":""},"PeriodicalIF":11.0000,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://nanoconvergencejournal.springeropen.com/counter/pdf/10.1186/s40580-025-00509-x","citationCount":"0","resultStr":"{\"title\":\"Efficient methods of isolation and purification of extracellular vesicles\",\"authors\":\"Taewoon Kim, Jong Wook Hong, Luke P. Lee\",\"doi\":\"10.1186/s40580-025-00509-x\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Living cells produce nanometer scales of extracellular vesicles (EVs) that have attracted considerable interest due to their transformative effects on diagnostics and therapies for cancer and other diseases. While significant advancements have been made in grasping the physical and chemical foundations of separation techniques for EVs, challenges must be overcome to ensure effective EV purification for diverse life sciences and clinical applications. This review highlights the most significant developments in efficient isolation and purification methods for EVs in transformative medicine. We examine the basic structure of exosomes and how to obtain specimens containing exosomes and EVs from various body fluids. We investigate the principles of physical, chemical, and biological isolation methods of EVs. We systematically evaluate different designs of microfluidics-based EV purification methods. We provide a comprehensive overview of the applications of exosomes in the life sciences and medicine. The precise engineering of EV isolation and purification generates a high yield and purity, offering practical solutions for translational medicine.</p><h3>Graphical Abstract</h3>\\n<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>\",\"PeriodicalId\":712,\"journal\":{\"name\":\"Nano Convergence\",\"volume\":\"12 1\",\"pages\":\"\"},\"PeriodicalIF\":11.0000,\"publicationDate\":\"2025-09-25\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://nanoconvergencejournal.springeropen.com/counter/pdf/10.1186/s40580-025-00509-x\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Nano Convergence\",\"FirstCategoryId\":\"88\",\"ListUrlMain\":\"https://link.springer.com/article/10.1186/s40580-025-00509-x\",\"RegionNum\":2,\"RegionCategory\":\"材料科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"MATERIALS SCIENCE, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Nano Convergence","FirstCategoryId":"88","ListUrlMain":"https://link.springer.com/article/10.1186/s40580-025-00509-x","RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"MATERIALS SCIENCE, MULTIDISCIPLINARY","Score":null,"Total":0}
Efficient methods of isolation and purification of extracellular vesicles
Living cells produce nanometer scales of extracellular vesicles (EVs) that have attracted considerable interest due to their transformative effects on diagnostics and therapies for cancer and other diseases. While significant advancements have been made in grasping the physical and chemical foundations of separation techniques for EVs, challenges must be overcome to ensure effective EV purification for diverse life sciences and clinical applications. This review highlights the most significant developments in efficient isolation and purification methods for EVs in transformative medicine. We examine the basic structure of exosomes and how to obtain specimens containing exosomes and EVs from various body fluids. We investigate the principles of physical, chemical, and biological isolation methods of EVs. We systematically evaluate different designs of microfluidics-based EV purification methods. We provide a comprehensive overview of the applications of exosomes in the life sciences and medicine. The precise engineering of EV isolation and purification generates a high yield and purity, offering practical solutions for translational medicine.
期刊介绍：
Nano Convergence is an internationally recognized, peer-reviewed, and interdisciplinary journal designed to foster effective communication among scientists spanning diverse research areas closely aligned with nanoscience and nanotechnology. Dedicated to encouraging the convergence of technologies across the nano- to microscopic scale, the journal aims to unveil novel scientific domains and cultivate fresh research prospects.
Operating on a single-blind peer-review system, Nano Convergence ensures transparency in the review process, with reviewers cognizant of authors' names and affiliations while maintaining anonymity in the feedback provided to authors.
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