利用电离辐射快速体外灭活多种SARS-CoV-2菌株:新的灭活模式和机制见解

IF 11.6 1区 工程技术 Q1 ENGINEERING, MULTIDISCIPLINARY
Wei Wang, Xiaodi Zhang, Jiageng Yu, Tianhao Weng, Zhiyang Yu, Zhigang Wu, Danrong Shi, Sufen Zhang, Xiangyun Lu, Osama Alam, Dahang Shen, Qian Bao, Qingfu Ye, Lanjuan Li, Hangping Yao
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引用次数: 0

摘要

电离辐射是一种重要的病毒灭活方法。然而,其对严重急性呼吸综合征冠状病毒2 (SARS-CoV-2)灭活的功效及其潜在机制尚不清楚。本研究利用20个野生型和突变型菌株验证了SARS-CoV-2的辐射敏感性和辐射诱导的生物学变化。结果表明,1.2 kGy的电子束照射或0.9 kGy的x射线照射可消除99.99%的SARS-CoV-2颗粒。与野生型相比,Delta和各种Omicron变体对辐射表现出更高的敏感性,在1.0和0.8 kGy下表现出近99.99%的失活效率。辐照剂量与病毒载量的对数减少之间的关系符合剂量-反应模型,其特征是窗口极窄。刺突(S)蛋白断裂,而不是普遍接受的核酸切割,被认为是主要的失活机制(触发S蛋白从融合前到融合后的构象转变,对核酸完整性的影响最小)。该研究引入了针对冠状病毒失活关键蛋白的概念,为传染性冠状病毒疾病控制和疫苗开发提供了有价值的见解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Rapid In-Vitro Inactivation of Various SARS-CoV-2 Strains Using Ionizing Radiation: New Inactivation Patterns And Mechanistic Insights
Ionizing radiation presents an important solution for virus inactivation. However, its efficacy for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) inactivation and the underlying mechanisms remain unclear. This study demonstrates radiosensitivity and radiation-induced biological changes in SARS-CoV-2 using 20 wild-type and mutant strains. The results show that 1.2 kGy of electron beam (E-beam) or 0.9 kGy of X-ray irradiation can eliminate 99.99% of SARS-CoV-2 particles. The Delta and various Omicron variants exhibit heightened sensitivity to radiation compared to the wild-type, showing nearly 99.99% inactivation efficiency at 1.0 and 0.8 kGy. The relationship between irradiation dose and the logarithmic reduction in virus load adheres to a dose–response model, characterized by extremely narrow windows. Spike (S) protein disruption, rather than the commonly accepted nucleic acid cleavage, is identified as the primary inactivation mechanism (triggering a conformation transition of S protein from pre-fusion to post-fusion with minimal impact on nucleic acid integrity). This study introduces the concept of targeting critical proteins in coronavirus inactivation, offering valuable insight for infectious coronavirus disease control and vaccine development.
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来源期刊
Engineering
Engineering Environmental Science-Environmental Engineering
自引率
1.60%
发文量
335
审稿时长
35 days
期刊介绍: Engineering, an international open-access journal initiated by the Chinese Academy of Engineering (CAE) in 2015, serves as a distinguished platform for disseminating cutting-edge advancements in engineering R&D, sharing major research outputs, and highlighting key achievements worldwide. The journal's objectives encompass reporting progress in engineering science, fostering discussions on hot topics, addressing areas of interest, challenges, and prospects in engineering development, while considering human and environmental well-being and ethics in engineering. It aims to inspire breakthroughs and innovations with profound economic and social significance, propelling them to advanced international standards and transforming them into a new productive force. Ultimately, this endeavor seeks to bring about positive changes globally, benefit humanity, and shape a new future.
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