使用双酸dna修饰的AuNP SPR探针对血清中环状rna的目视计数

IF 3.3 3区化学 Q2 CHEMISTRY, ANALYTICAL

Analyst Pub Date : 2025-09-25 DOI:10.1039/d5an00875a

Yuanzhao Shen, Ran Wan, Xin Zhou

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引用次数: 0

摘要

本研究建立了一个60 nm双酸dna修饰的Au纳米颗粒（AuNP）平台，用于暗场显微镜（DFM）快速检测circRNA。与其他Au纳米结构相比，球形AuNPs表现出优异的稳定性和灵敏度，在40分钟内对circHECTD1的检测限为~0.02 fM，比qPCR方法对该靶标的灵敏度提高了约100倍。这种方法可以实现快速、经济、高灵敏度的circRNA分析，而无需PCR扩增。

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Visual enumeration of circRNA in serum using a biserrate DNA-modified AuNP SPR probe

This study developed a 60 nm biserrate DNA-modified Au nanoparticle (AuNP) platform for rapid circRNA detection using dark-field microscopy (DFM). Compared with other Au nanostructures, spherical AuNPs exhibited superior stability and sensitivity, achieving a detection limit of ~0.02 fM for circHECTD1 within 40 min, representing about a 100-fold improvement in sensitivity over qPCR method for this target. This approach enables fast, cost-effective, and highly sensitive circRNA analysis without PCR amplification.

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