{"title":"利用rt - pcr测序方法分离和鉴定导致印度尼西亚2011 - 2018年禽蛋产量下降的H9N2病毒。","authors":"Kadek Rachmawati, Kuncoro Puguh Santoso, Rochmah Kurnijasanti, Aswin Rafif Khairullah","doi":"10.5455/OVJ.2025.v15.i6.50","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Influenza A virus subtype H9 is enzootic in portions of North and Central Africa, the Middle East, and Asia, where it significantly reduces the chicken industry's profitability. The H9N2 virus was reported in Indonesia in late 2016, causing decreased egg production and increased mortality. Research on the H9N2 virus is important as an early warning system for the reassortment of potentially pandemic viruses such as HPAI virus subtype H5N1.</p><p><strong>Aim: </strong>This tracking study aimed to isolate and identify the H9N2 virus in laying hens from samples collected from 2011 to 2018 in Indonesia.</p><p><strong>Methods: </strong>Sequencing was performed on 2,949 samples obtained from tracheal and cloacal swabs after examination for the H9N2 virus using real-time reverse transcriptase-polymerase chain reaction.</p><p><strong>Results: </strong>One positive sample was from West Java in 2017. The results of the phylogenetic analysis revealed that this virus belongs to the China-Vietnam-Indonesia lineage and is related to the A/chicken/West Java/VSN791/2017(H9N2) and A/chicken/Viet Nam/QN-2576/2015(H9N2) viruses.</p><p><strong>Conclusion: </strong>As an early warning system for AI outbreaks, active surveillance is required to track the progress of LPAI viruses like H9N2.</p>","PeriodicalId":19531,"journal":{"name":"Open Veterinary Journal","volume":"15 6","pages":"2815-2822"},"PeriodicalIF":1.0000,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12451140/pdf/","citationCount":"0","resultStr":"{\"title\":\"Isolation and identification of the H9N2 virus that caused the decline in poultry egg production in Indonesia from 2011 to 2018 using the RT-PCR-sequencing method.\",\"authors\":\"Kadek Rachmawati, Kuncoro Puguh Santoso, Rochmah Kurnijasanti, Aswin Rafif Khairullah\",\"doi\":\"10.5455/OVJ.2025.v15.i6.50\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Influenza A virus subtype H9 is enzootic in portions of North and Central Africa, the Middle East, and Asia, where it significantly reduces the chicken industry's profitability. The H9N2 virus was reported in Indonesia in late 2016, causing decreased egg production and increased mortality. Research on the H9N2 virus is important as an early warning system for the reassortment of potentially pandemic viruses such as HPAI virus subtype H5N1.</p><p><strong>Aim: </strong>This tracking study aimed to isolate and identify the H9N2 virus in laying hens from samples collected from 2011 to 2018 in Indonesia.</p><p><strong>Methods: </strong>Sequencing was performed on 2,949 samples obtained from tracheal and cloacal swabs after examination for the H9N2 virus using real-time reverse transcriptase-polymerase chain reaction.</p><p><strong>Results: </strong>One positive sample was from West Java in 2017. The results of the phylogenetic analysis revealed that this virus belongs to the China-Vietnam-Indonesia lineage and is related to the A/chicken/West Java/VSN791/2017(H9N2) and A/chicken/Viet Nam/QN-2576/2015(H9N2) viruses.</p><p><strong>Conclusion: </strong>As an early warning system for AI outbreaks, active surveillance is required to track the progress of LPAI viruses like H9N2.</p>\",\"PeriodicalId\":19531,\"journal\":{\"name\":\"Open Veterinary Journal\",\"volume\":\"15 6\",\"pages\":\"2815-2822\"},\"PeriodicalIF\":1.0000,\"publicationDate\":\"2025-06-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12451140/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Open Veterinary Journal\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.5455/OVJ.2025.v15.i6.50\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/6/30 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q3\",\"JCRName\":\"VETERINARY SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Open Veterinary Journal","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5455/OVJ.2025.v15.i6.50","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/6/30 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}
Isolation and identification of the H9N2 virus that caused the decline in poultry egg production in Indonesia from 2011 to 2018 using the RT-PCR-sequencing method.
Background: Influenza A virus subtype H9 is enzootic in portions of North and Central Africa, the Middle East, and Asia, where it significantly reduces the chicken industry's profitability. The H9N2 virus was reported in Indonesia in late 2016, causing decreased egg production and increased mortality. Research on the H9N2 virus is important as an early warning system for the reassortment of potentially pandemic viruses such as HPAI virus subtype H5N1.
Aim: This tracking study aimed to isolate and identify the H9N2 virus in laying hens from samples collected from 2011 to 2018 in Indonesia.
Methods: Sequencing was performed on 2,949 samples obtained from tracheal and cloacal swabs after examination for the H9N2 virus using real-time reverse transcriptase-polymerase chain reaction.
Results: One positive sample was from West Java in 2017. The results of the phylogenetic analysis revealed that this virus belongs to the China-Vietnam-Indonesia lineage and is related to the A/chicken/West Java/VSN791/2017(H9N2) and A/chicken/Viet Nam/QN-2576/2015(H9N2) viruses.
Conclusion: As an early warning system for AI outbreaks, active surveillance is required to track the progress of LPAI viruses like H9N2.
期刊介绍：
Open Veterinary Journal is a peer-reviewed international open access online and printed journal that publishes high-quality original research articles. reviews, short communications and case reports dedicated to all aspects of veterinary sciences and its related subjects. Research areas include the following: Infectious diseases of zoonotic/food-borne importance, applied biochemistry, parasitology, endocrinology, microbiology, immunology, pathology, pharmacology, physiology, epidemiology, molecular biology, immunogenetics, surgery, ophthalmology, dermatology, oncology and animal reproduction. All papers are peer-reviewed. Moreover, with the presence of well-qualified group of international referees, the process of publication will be done meticulously and to the highest standards.
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