Ga-Yeong Lee, Hae-Yeon Cho, Syed Al Jawad Sayem, Seung-Joon Kim, Md Sekendar Ali, Seung-Chun Park
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The EzBioCloud software was used to perform gene assembly, and the genes were interpreted by the National Center for Biotechnology Information (NCBI) and the Glimmer program. Core and pan-genomic analyses were performed to assess the extent of functional conservation in the genomic sequence. Moreover, the NCBI database and the Basic Local Alignment Search Tool software were used to identify antimicrobial resistance genes and virulence factors.</p><p><strong>Results: </strong>Limosilactobacillus reuteri PSC102 consists of a single circular chromosome with 2,048,626 bp, a guanine- cytosine of 38.9%, 18 rRNA genes, and 69 tRNA genes. Among the 1,846 protein-coding sequences, genes associated with probiotic characteristics were identified, including genes involved in host-microbe interactions, stress tolerance, biogenesis, and defense mechanisms. Furthermore, the genome of L. reuteri PSC102 comprises 2,446 pan-genome and 1,222 core-genome orthologous gene clusters. A total of 74 unique genes were identified in L. reuteri PSC102 genome. These genes mostly encode proteins potentially involved in the transport and metabolism of amino acids and carbohydrates. Moreover, antibacterial resistance genes and virulence factors were absent in L. reuteri PSC102.</p><p><strong>Conclusion: </strong>The results of the molecular insight into <i>L. reuteri</i> PSC102 corroborates its use as a probiotic in humans and other animals.</p>","PeriodicalId":19531,"journal":{"name":"Open Veterinary Journal","volume":"15 6","pages":"2427-2438"},"PeriodicalIF":1.0000,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12451167/pdf/","citationCount":"0","resultStr":"{\"title\":\"Complete genome sequence and genomic characterization of the probiotic <i>Limosilactobacillus reuteri</i> PSC102.\",\"authors\":\"Ga-Yeong Lee, Hae-Yeon Cho, Syed Al Jawad Sayem, Seung-Joon Kim, Md Sekendar Ali, Seung-Chun Park\",\"doi\":\"10.5455/OVJ.2025.v15.i6.15\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Gut microbiota are potential sources of probiotics and play an essential role in maintaining intestinal health. <i>Limosilactobacillus reuteri</i> PSC102 (<i>L. reuteri</i> PSC102), which was isolated from the feces of healthy pigs, exhibited health-beneficial properties.</p><p><strong>Aim: </strong>We aimed to conduct a whole-genome sequencing analysis of <i>L. reuteri</i> PSC102 to determine its molecular characteristics as a probiotic strain.</p><p><strong>Methods: </strong><i>Limosilactobacillus reuteri</i> PSC102 cells were cultured in De Man-Rogosa-Sharpe medium, followed by DNA extraction for genomic analysis using the PacBio-Illumina sequencing platform. 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引用次数: 0
摘要
背景：肠道菌群是益生菌的潜在来源，在维持肠道健康中起着重要作用。从健康猪的粪便中分离得到罗伊氏乳杆菌PSC102 (L. reuteri PSC102)，具有有益健康的特性。目的：对罗伊氏乳杆菌PSC102进行全基因组测序分析，以确定其作为益生菌菌株的分子特征。方法：在De Man-Rogosa-Sharpe培养基中培养罗伊氏芽孢杆菌PSC102细胞，提取DNA，利用PacBio-Illumina测序平台进行基因组分析。使用EzBioCloud软件进行基因组装，基因由国家生物技术信息中心（NCBI）和Glimmer程序进行解释。进行核心和泛基因组分析以评估基因组序列中功能保护的程度。此外，利用NCBI数据库和Basic Local Alignment Search Tool软件鉴定耐药基因和毒力因子。结果：罗伊氏乳酸杆菌PSC102由一条长2,048,626 bp的单圆形染色体组成，鸟嘌呤-胞嘧啶占38.9%，有18个rRNA基因和69个tRNA基因。在1846个蛋白质编码序列中，鉴定出与益生菌特性相关的基因，包括参与宿主-微生物相互作用、胁迫耐受性、生物发生和防御机制的基因。此外，罗伊氏乳杆菌PSC102基因组包括2446个泛基因组和1222个核心基因组同源基因簇。在罗伊氏乳杆菌PSC102基因组中共鉴定出74个独特基因。这些基因大多编码可能参与氨基酸和碳水化合物运输和代谢的蛋白质。此外，罗伊氏乳杆菌PSC102缺乏抗菌耐药基因和毒力因子。结论：对罗伊氏乳杆菌PSC102的分子研究结果证实了其作为益生菌在人类和其他动物中的应用。
Complete genome sequence and genomic characterization of the probiotic Limosilactobacillus reuteri PSC102.
Background: Gut microbiota are potential sources of probiotics and play an essential role in maintaining intestinal health. Limosilactobacillus reuteri PSC102 (L. reuteri PSC102), which was isolated from the feces of healthy pigs, exhibited health-beneficial properties.
Aim: We aimed to conduct a whole-genome sequencing analysis of L. reuteri PSC102 to determine its molecular characteristics as a probiotic strain.
Methods: Limosilactobacillus reuteri PSC102 cells were cultured in De Man-Rogosa-Sharpe medium, followed by DNA extraction for genomic analysis using the PacBio-Illumina sequencing platform. The EzBioCloud software was used to perform gene assembly, and the genes were interpreted by the National Center for Biotechnology Information (NCBI) and the Glimmer program. Core and pan-genomic analyses were performed to assess the extent of functional conservation in the genomic sequence. Moreover, the NCBI database and the Basic Local Alignment Search Tool software were used to identify antimicrobial resistance genes and virulence factors.
Results: Limosilactobacillus reuteri PSC102 consists of a single circular chromosome with 2,048,626 bp, a guanine- cytosine of 38.9%, 18 rRNA genes, and 69 tRNA genes. Among the 1,846 protein-coding sequences, genes associated with probiotic characteristics were identified, including genes involved in host-microbe interactions, stress tolerance, biogenesis, and defense mechanisms. Furthermore, the genome of L. reuteri PSC102 comprises 2,446 pan-genome and 1,222 core-genome orthologous gene clusters. A total of 74 unique genes were identified in L. reuteri PSC102 genome. These genes mostly encode proteins potentially involved in the transport and metabolism of amino acids and carbohydrates. Moreover, antibacterial resistance genes and virulence factors were absent in L. reuteri PSC102.
Conclusion: The results of the molecular insight into L. reuteri PSC102 corroborates its use as a probiotic in humans and other animals.
期刊介绍：
Open Veterinary Journal is a peer-reviewed international open access online and printed journal that publishes high-quality original research articles. reviews, short communications and case reports dedicated to all aspects of veterinary sciences and its related subjects. Research areas include the following: Infectious diseases of zoonotic/food-borne importance, applied biochemistry, parasitology, endocrinology, microbiology, immunology, pathology, pharmacology, physiology, epidemiology, molecular biology, immunogenetics, surgery, ophthalmology, dermatology, oncology and animal reproduction. All papers are peer-reviewed. Moreover, with the presence of well-qualified group of international referees, the process of publication will be done meticulously and to the highest standards.
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