伏隔核APP/PS1神经元培养模型中神经元兴奋性和ampa介导的神经传递的改变。

IF 3.1 3区医学 Q2 NEUROSCIENCES

Journal of Alzheimer's Disease Pub Date : 2025-09-24 DOI:10.1177/13872877251378468

Paulina Saavedra-Sieyes, Isaías Meza, Nicolás Riffo-Lepe, Loreto San Martin, Juliana González-SanMiguel, Luis G Aguayo

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引用次数: 0

摘要

阿尔茨海默病是一种神经退行性疾病。最近，中边缘系统的改变已经被描述，特别是在伏隔核（nAc）。我们发现，APP/PS1小鼠在体外培养10天和15天时，细胞内Aβ的积累量更高。在离体15天（DIV）， APP/PS1伏隔神经元突触传递和神经元兴奋性发生改变。具体来说，AMPA能突触电流增加，这对NASPM的应用很敏感，表明钙通透性AMPA受体（CP-AMPARs）发挥了关键作用。我们的研究表明，这种神经元培养模型准确地复制了阿尔茨海默病早期的变化。

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Alterations in neuronal excitability and AMPA-mediated neurotransmission in an APP/PS1 neuronal culture model derived from nucleus accumbens.

Alzheimer's disease is a neurodegenerative disease. Recently, alterations in the mesolimbic system have been described, especially in the nucleus accumbens (nAc). We found that intracellular accumulation of Aβ was higher in neurons of the APP/PS1 mice at 10 and 15 days in vitro. At 15 days in vitro (DIV), APP/PS1 accumbal neurons showed alterations in synaptic transmission and neuronal excitability. Specifically, there was an increase in AMPAergic synaptic currents, which was sensitive to the application of NASPM, suggesting a critical role for calcium-permeable AMPA receptors (CP-AMPARs). Our study indicates that this neuronal culture model accurately replicates alterations in the early stages of AD.

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来源期刊

Journal of Alzheimer's Disease 医学-神经科学

CiteScore

6.40

自引率

7.50%

发文量

1327

审稿时长

2 months

期刊介绍： The Journal of Alzheimer''s Disease (JAD) is an international multidisciplinary journal to facilitate progress in understanding the etiology, pathogenesis, epidemiology, genetics, behavior, treatment and psychology of Alzheimer''s disease. The journal publishes research reports, reviews, short communications, hypotheses, ethics reviews, book reviews, and letters-to-the-editor. The journal is dedicated to providing an open forum for original research that will expedite our fundamental understanding of Alzheimer''s disease.