老年Fischer 344大鼠心脏巨噬细胞的免疫组化评价。

IF 1.5 4区 生物学 Q4 CELL BIOLOGY
Steven A Bloomer
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引用次数: 0

摘要

巨噬细胞在心脏中具有多种作用,包括免疫监视和细胞外基质重塑。衰老增加了心脏中胶原沉积和巨噬细胞的数量;然而,用于研究心脏巨噬细胞的啮齿动物模型具有与年龄相关的合并症,如动脉粥样硬化和高血压。Fischer 344大鼠不会随着年龄的增长而出现这些情况;因此,本研究的目的是评估老龄(24月龄)和幼龄(6月龄)Fischer 344大鼠心脏中巨噬细胞的数量和极化情况。对石蜡包埋的心脏进行胶原沉积评估,并对CD68、CD163、CD206和半凝集素-3进行免疫标记。与年轻大鼠相比,老年大鼠胶原沉积明显增加。CD68+和CD163+细胞在不同年龄组间无显著差异,但CD206+和半乳糖凝集素-3+细胞在老年动物中数量更多。双免疫荧光研究表明,半乳糖凝集素-3可与CD68和CD163共定位,提示半乳糖凝集素-3存在于心脏巨噬细胞中。进一步的共定位研究表明,CD68+/CD163-、CD68+/CD163+和CD68-/CD163+细胞在不同年龄组之间的比例相似,表明衰老不影响巨噬细胞极化。由于CD206+和半乳糖凝集素-3+细胞促进纤维化,这些结果为未来研究描绘这些细胞在衰老心脏中的特定作用提供了依据。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Immunohistochemical Assessment of Cardiac Macrophages in the Aged Fischer 344 Rat.

Macrophages have multiple roles in the heart including immune surveillance and extracellular matrix remodeling. Aging increases both collagen deposition and macrophage number in the heart; however, rodent models used to study cardiac macrophages have age-related comorbidities such as atherosclerosis and hypertension. The Fischer 344 rat does not develop these conditions with aging; therefore, the purpose of this study was to evaluate macrophage number and polarization in the hearts of aged (24-month) and young (6-month) Fischer 344 rats. Paraffin-embedded hearts were assessed for collagen deposition and immunolabeled for CD68, CD163, CD206, and galectin-3. Compared with young rats, significantly greater collagen deposition was observed in the old rats. There were no significant differences in CD68+ or CD163+ cells between age groups, but both CD206+ and galectin-3+ cells were more numerous in the aged animals. Double-immunofluorescence studies demonstrated that galectin-3 colocalized with both CD68 and CD163, suggesting that galectin-3 is found in cardiac macrophages. Further colocalization studies demonstrated similar proportions of CD68+/CD163-, CD68+/CD163+, and CD68-/CD163+ cells between age groups, suggesting that aging does not affect macrophage polarization. As CD206+ and galectin-3+ cells promote fibrosis, these results warrant future studies that delineate the specific roles of these cells in the aged heart.

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来源期刊
CiteScore
6.00
自引率
0.00%
发文量
32
审稿时长
1 months
期刊介绍: Journal of Histochemistry & Cytochemistry (JHC) has been a pre-eminent cell biology journal for over 50 years. Published monthly, JHC offers primary research articles, timely reviews, editorials, and perspectives on the structure and function of cells, tissues, and organs, as well as mechanisms of development, differentiation, and disease. JHC also publishes new developments in microscopy and imaging, especially where imaging techniques complement current genetic, molecular and biochemical investigations of cell and tissue function. JHC offers generous space for articles and recognizing the value of images that reveal molecular, cellular and tissue organization, offers free color to all authors.
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