电化学分析揭示了Coelenterazine对去质子化Coelenteramide的化学发光过程

IF 2.3 3区化学 Q2 CHEMISTRY, ANALYTICAL

Electroanalysis Pub Date : 2025-08-07 DOI:10.1002/elan.70029

Yoshihiro Ohmiya, Ryo Nishihara, Tomoyuki Kamata, Michinori Sumimoto, Dai Kato

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引用次数: 0

摘要

Coelenterazine是海洋生物发光生物中最常见的咪唑吡嗪类荧光素。在循环伏安曲线上，在约0.12和0.62 v处观察到复方肠菌嗪的电化学主峰。理论分析表明，coelenterazine在0.12 V左右转化为coelenteramide， coelenteramide在0.62 V左右脱质子。这些方法可以揭示腔肠酰胺可能的激发态，但不能始终如一地使酶促生物发光反应被追踪。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Electrochemical Analysis Reveals the Chemiluminescence Processes of Coelenterazine to Deprotonated Coelenteramide

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Electrochemical Analysis Reveals the Chemiluminescence Processes of Coelenterazine to Deprotonated Coelenteramide

Coelenterazine is the most common imidazopyrazinone-based luciferin in marine bioluminescence organisms. The electrochemical main peaks of coelenterazine are observed at approximately around 0.12 and 0.62 Vs in the cyclic voltammetry curve. Theoretical analysis suggests that coelenterazine is converted to the coelenteramide around 0.12 V, and the coelenteramide is deprotonated at around 0.62 V. These approaches can reveal the possible excited states of coelenteramide but do not consistently enable the enzymatic bioluminescence reaction to be traced.

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来源期刊

Electroanalysis 化学-电化学

CiteScore

6.00

自引率

3.30%

发文量

222

审稿时长

2.4 months

期刊介绍： Electroanalysis is an international, peer-reviewed journal covering all branches of electroanalytical chemistry, including both fundamental and application papers as well as reviews dealing with new electrochemical sensors and biosensors, nanobioelectronics devices, analytical voltammetry, potentiometry, new electrochemical detection schemes based on novel nanomaterials, fuel cells and biofuel cells, and important practical applications. Serving as a vital communication link between the research labs and the field, Electroanalysis helps you to quickly adapt the latest innovations into practical clinical, environmental, food analysis, industrial and energy-related applications. Electroanalysis provides the most comprehensive coverage of the field and is the number one source for information on electroanalytical chemistry, electrochemical sensors and biosensors and fuel/biofuel cells.