Tiziana Raia, Rosaria A. Cavallaro, Luiza Diniz Ferreira Borges, Stefano Cinti, Mariano Bizzarri, Isidre Ferrer, Marco Lucarelli, Andrea Fuso
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Finally, we assessed <i>miR-29a</i> expression and methylation in the brain of AD subjects.</p>\n </section>\n \n <section>\n \n <h3> RESULTS</h3>\n \n <p><i>MiR-29a</i> was repressed in hypomethylating and expressed in hypermethylating conditions. The expression of <i>miR-29a</i> and of its target, <i>BACE1</i>, was inversely correlated.</p>\n </section>\n \n <section>\n \n <h3> DISCUSSION</h3>\n \n <p>We demonstrated for the first time that <i>miR-29a</i> is modulated by one-carbon metabolism through DNA methylation, disclosing the molecular mechanisms regulating <i>BACE1</i> expression in AD. These data confirm <i>miR-29a</i>’s protective role in AD and support <i>miR-29a</i> as a potential biomarker for AD.</p>\n </section>\n </div>","PeriodicalId":7471,"journal":{"name":"Alzheimer's & Dementia","volume":"21 9","pages":""},"PeriodicalIF":11.1000,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://alz-journals.onlinelibrary.wiley.com/doi/epdf/10.1002/alz.70703","citationCount":"0","resultStr":"{\"title\":\"One-carbon metabolism modulates miR-29a–DNA methylation crosstalk in Alzheimer's disease\",\"authors\":\"Tiziana Raia, Rosaria A. 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Finally, we assessed <i>miR-29a</i> expression and methylation in the brain of AD subjects.</p>\\n </section>\\n \\n <section>\\n \\n <h3> RESULTS</h3>\\n \\n <p><i>MiR-29a</i> was repressed in hypomethylating and expressed in hypermethylating conditions. The expression of <i>miR-29a</i> and of its target, <i>BACE1</i>, was inversely correlated.</p>\\n </section>\\n \\n <section>\\n \\n <h3> DISCUSSION</h3>\\n \\n <p>We demonstrated for the first time that <i>miR-29a</i> is modulated by one-carbon metabolism through DNA methylation, disclosing the molecular mechanisms regulating <i>BACE1</i> expression in AD. 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One-carbon metabolism modulates miR-29a–DNA methylation crosstalk in Alzheimer's disease
INTRODUCTION
Alzheimer's disease (AD)’s multifactorial nature stresses the role of epigenetics in affecting different pathological pathways. We demonstrated that one-carbon metabolism epigenetically impacts AD-like phenotype. Here, we investigated the crosstalk between methylation and microRNAs in AD.
METHODS
We altered one-carbon metabolism to induce hypo- and hyper-methylation, in SK-N-BE neuroblastoma cells and TgCRND8 mice. miRNAs were profiled through a polymerase chain reaction array, then we focused on miR-29a expression and methylation of its genomic locus. Finally, we assessed miR-29a expression and methylation in the brain of AD subjects.
RESULTS
MiR-29a was repressed in hypomethylating and expressed in hypermethylating conditions. The expression of miR-29a and of its target, BACE1, was inversely correlated.
DISCUSSION
We demonstrated for the first time that miR-29a is modulated by one-carbon metabolism through DNA methylation, disclosing the molecular mechanisms regulating BACE1 expression in AD. These data confirm miR-29a’s protective role in AD and support miR-29a as a potential biomarker for AD.
期刊介绍：
Alzheimer's & Dementia is a peer-reviewed journal that aims to bridge knowledge gaps in dementia research by covering the entire spectrum, from basic science to clinical trials to social and behavioral investigations. It provides a platform for rapid communication of new findings and ideas, optimal translation of research into practical applications, increasing knowledge across diverse disciplines for early detection, diagnosis, and intervention, and identifying promising new research directions. In July 2008, Alzheimer's & Dementia was accepted for indexing by MEDLINE, recognizing its scientific merit and contribution to Alzheimer's research.
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